Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Malachite green hapten, malachite green artificial antigen and preparation method of malachite green hapten and artificial antigen

A leuco-malachite green, artificial antigen technology, applied in the field of immunochemistry, can solve the problems of weak antibody specificity and sensitivity, low recovery rate, etc.

Active Publication Date: 2013-04-03
WUXI ZODOLABS BIOTECH
View PDF6 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] However, the above inventions are the same as the prior art, and their antibody specificity and sensitivity are weak, and the recovery rate is too low during detection, which cannot meet the existing needs.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Malachite green hapten, malachite green artificial antigen and preparation method of malachite green hapten and artificial antigen
  • Malachite green hapten, malachite green artificial antigen and preparation method of malachite green hapten and artificial antigen
  • Malachite green hapten, malachite green artificial antigen and preparation method of malachite green hapten and artificial antigen

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] Take 2.5g (20mmol) of N,N-dimethylaniline into a 50mL two-necked flask, then slowly add 20mL of 5% Amberlyst15 resin toluene solution, stir magnetically for a few minutes, and add 1.50g p-formylbenzene under stirring Formic acid or m-formylbenzoic acid, stirred overnight at 80~100°C. After the reaction was completed, the catalyst was filtered, and saturated sodium bicarbonate solution was added to the reaction system to adjust the pH to 9.0, and extracted with dichloromethane. The pH of the aqueous phase was adjusted to 2.0 with dilute hydrochloric acid, extracted with ethyl acetate, and the organic phase was spun to dryness to obtain a crude product. Then add 2g of HATU, 0.5g of ethylenediamine, 2ml of DIPEA, dissolve with 20mL of DMF, stir with magnetic force at 50°C for 2h, add water to quench and extract with ethyl acetate, spin the organic phase to dryness, and finally use vacuum silica gel column chromatography Separation and purification yielded the desired prod...

Embodiment 2

[0076] Take 2.5g (20mmol) of N,N-dimethylaniline into a 50mL two-necked flask, slowly add 20mL of 5% Amberlyst15 resin toluene solution, stir magnetically for a few minutes, and add 1.50g of the compound of formula (XI) under stirring (where R 6 for CONH 2 , R 7 for H, or R 6 for H, R 7 for CONH 2 ), and stirred overnight at 80~100°C. After the reaction was completed, the catalyst was filtered, and saturated sodium bicarbonate solution was added to the reaction system to adjust the pH to 9.0, extracted with ethyl acetate, and the organic phase was rotary evaporated and spin-dried to obtain a crude product. Then dissolve in 10mL THF, add 1.5g Lithium Aluminum Hydride, stir the reaction with magnetic force at 0°C for 2h, quench with ammonium chloride solution, adjust the pH to 9.0, extract with ethyl acetate, spin the organic phase to dryness, and finally The desired compound was separated and purified by silica gel column chromatography. Then dissolve it with 20 mL of DM...

Embodiment 3

[0081] Take 2.5g (20mmol) of N,N-dimethylaniline into a 50mL two-necked flask, then slowly add 20mL of 5% Amberlyst15 resin toluene solution, stir magnetically for a few minutes, and add 1.50g p-formylbenzene under stirring Formic acid or m-formylbenzoic acid, stirred overnight at 80~100°C. After the reaction was completed, the catalyst was filtered and saturated sodium bicarbonate solution was added to the reaction system to adjust the pH to 9.0, and extracted with dichloromethane. The pH of the aqueous phase was adjusted to 2.0 with dilute hydrochloric acid, extracted with ethyl acetate, and the organic phase was spun to dryness to obtain a crude product. Then add 2g of HATU, 2g of the compound of formula (g), 2mL of DIPEA, dissolve in 20mL of DMF, stir with magnetic force at 50°C for 2h, add water to quench and extract with ethyl acetate, spin the organic phase to dryness, and finally use decompression silica gel The desired product was obtained by separation and purificat...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses malachite green hapten, malachite green artificial antigen and preparation methods of the malachite green hapten and artificial antigen. The malachite green hapten retains the feature structure of malachite green, and has active arms which are derived from different microdomains of the structure of malachite green, have different electron cloud densities and different carbon chain lengths, are suitable to be coupled with macromolecule carriers, and are amide linkage with amino or carboxyl tail end. The malachite green artificial antigen prepared with leucomalachite green hapten has very high specificity and sensitivity when applied to detection of the residual quantity of leucomalachite green and malachite green, and is high in accuracy and recovery. Moreover, the methods are simple, the cost is low, and a fast, sensitive, economical and efficient malachite green immunodetection method is established.

Description

technical field [0001] The invention belongs to the technical field of immunochemistry and relates to the preparation of antigens, in particular to a leuco malachite green hapten, an artificial antigen and a preparation method. Background technique [0002] Malachite Green (Malachite Green, MG), also known as China Green, Aniline Green, Alkaline Malachite Green, Magenta Green, Salt-based Block Green, etc., is a toxic triphenylmethane compound that used to be used as a topical insect repellant , fungicides, bactericides, etc. are used to treat parasitic, fungal or bacterial infections in fish or fish eggs. However, malachite green is also a potential carcinogen, mutagen, and mutagen. Many countries in the world have listed malachite green as a banned drug for aquaculture. Leucomalachite Green (Leucomalachite Green, LMG) is the chromophore-free reduction product of malachite green. It is the main metabolite and existing form of malachite green in fish and other organisms. It ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07C237/34C07C233/36C07C231/02C07K14/765G01N33/577
CPCC09B11/12
Inventor 卞禹卜叶帮伟高君金晶刘春梅沈智勇杨晓慧李林
Owner WUXI ZODOLABS BIOTECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com