Mercury ion colloidal gold colorimetric detection method and mercury ion detection kit

A technology of colloidal gold and mercury ions, which is applied in the field of biochemistry, can solve the problems of high cost, difficult preparation of nucleic acid unaccompanied bodies, and complicated operation, and achieve the effects of low cost, good stability, and fast sensitivity

Inactive Publication Date: 2014-07-02
HEFEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method requires surface modification of gold nanoparticles, which is complicated to operate, and the preparation of nucleic acid anaphylaxis is difficult and expensive.

Method used

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  • Mercury ion colloidal gold colorimetric detection method and mercury ion detection kit
  • Mercury ion colloidal gold colorimetric detection method and mercury ion detection kit
  • Mercury ion colloidal gold colorimetric detection method and mercury ion detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 The Mercury ion colloidal gold colorimetric detection method comprises the following steps:

[0030] (1) Prepare the sodium citrate-wrapped gold nanoparticle solution with the sodium citrate reduction method;

[0031] Boil 0.1% chloroauric acid until boiling, then quickly add 1% sodium citrate under vigorous stirring, until the color of the solution turns bright red, continue to boil for 5 minutes to stabilize the gold nanoparticle solution.

[0032] (2) adding Hg in the gold nanoparticle solution prepared in step (1) 2+ , by electrostatic adsorption to Hg 2+ immobilized on the surface of gold nanoparticles;

[0033] Concentrate the gold nanoparticle solution prepared in step (1) to 4 times by centrifugation (9400 xG), take 7 centrifuge tubes (No. 1, 2, 3, 4, 5, 6, 7, 8), add 80 µL of 4-fold concentrated gold nanoparticle solution and 10 µL of Hg of different concentrations 2+ solution so that Hg 2+ The final concentrations were 0, 0.01, 0.02, 0.05, 0.1...

Embodiment 2

[0038] Example 2 Referring to steps (1)-(2) of Example 1, the prepared gold nanoparticle solution was concentrated to 4 times by centrifugation (9400 xG), and 7 centrifuge tubes (No. 1, 2, 3, 4, 5, 6 , 7, 8), 80 μL of 4-fold concentrated gold nanoparticle solution and 10 μL of different concentrations of Hg were added to each tube 2+ solution so that Hg 2+ The final concentrations were 0, 0.01, 0.02, 0.05, 0.1, 0.2 and 0.5 nM, reacted for 5 min, and Hg 2+ immobilized on the surface of gold nanoparticles.

[0039] Then 10 μL of 0.4 mM lysine was added to each of the aforementioned solutions, so that the Hg adsorbed on the surface of gold nanoparticles 2+ and both ends of lysine -NH 2 The formation of N-Hg-N structure enables the aggregation of gold nanoparticles. Use a UV-visible spectrophotometer to measure the UV spectrum of each tube solution from 200 nm to 850 nm, and according to A 700 / A 520 A standard curve was drawn. The result is as Figure 1-Figure 2 shown. ...

Embodiment 3

[0040] Example 3 Take 10 centrifuge tubes (No. 1, 2, 3, 4, 5, 6, 7, 8, 9, 10), and add 80 μL of 4-fold concentrated gold nanoparticle solution and 10 μL of different metal ion solutions (Ag + , K + , Ca 2+ , Pb 2+ , Ba 2+ , Ni 2+ , Zn 2+ , Mg 2+ , Al 3+ 100 nM; Hg 2+ 1 nM), reacted for 5 min, and fixed the metal ions on the surface of gold nanoparticles by electrostatic adsorption.

[0041] Then add 10 μL of 0.4 mM lysine to the solution obtained in the previous step, and observe the color of the solution. Use a UV-visible spectrophotometer to measure the UV spectrum of each tube solution from 200 nm to 850 nm, and according to A 700 / A 520 Draw a histogram of the ratio of . The result is as Figure 3-Figure 4 shown.

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Abstract

The invention discloses a mercury ion colloidal gold colorimetric detection method and a mercury ion detection kit. According to the mercury ion colloidal gold colorimetric detection method, mercury ions are adsorbed to a gold nano particle solution (colloidal gold) wrapped with citric acid ions, and then lysine capable of specifically identifying the mercury ions is added to form a bridge, so that gold nano particles are gathered, and the mercury ions can be quickly detected on the spot according to the color change. The lysine serving as an important reagent is low in cost and readily available, so that the cost is greatly reduced; furthermore, the surfaces of the gold nano particles are not required to be modified, so that the method is convenient and quick. The mercury ion colloidal gold colorimetric detection method has the advantages of quickness, high sensitivity, high specificity, high stability, low cost and the like and is easy to popularize and conventionally apply.

Description

technical field [0001] The present invention relates to a Hg 2+ Detection method, in particular to a rapid detection of heavy metal Hg 2+ The gold standard colorimetric method belongs to the technical field of biochemistry. technical background [0002] Mercury widely exists in nature. Various natural phenomena (rain, snow, etc.) can make mercury circulate continuously on the surface, air and water, and can be absorbed by animals and plants. Under the biomagnification of the food chain, they are enriched by thousands of times, and finally enter the human body, causing heavy metal poisoning, ranging from strange diseases (Minamata disease, bone pain, etc.), to severe cases leading to death. With the development of industry, a large amount of mercury is discharged into nature without treatment, making mercury pollution quite serious. [0003] The most studied heavy metal Hg 2+ Rapid detection methods mainly include: atomic fluorescence spectrometry, inductively coupled pla...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
Inventor 陈伟邓怡潘道东姚丽
Owner HEFEI UNIV OF TECH
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