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Mabuterol colloidal gold test strip and preparation method thereof

A colloidal gold test paper, colloidal gold technology, applied in chemical instruments and methods, specific peptides, animal/human proteins, etc., can solve problems that need to be improved, and achieve low cost, accurate results, and long storage time.

Inactive Publication Date: 2013-06-12
江西中德生物工程股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Therefore, the current detection methods for Mabuterol still need to be improved

Method used

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  • Mabuterol colloidal gold test strip and preparation method thereof
  • Mabuterol colloidal gold test strip and preparation method thereof
  • Mabuterol colloidal gold test strip and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Embodiment 1 complete antigen (immunogen) synthesis

[0044]

[0045] Solution A: Weigh 50mg of Mabuterol, dissolve it with 10mL of distilled water with pH1.0; Solution B: Weigh out 60mg of sodium nitrite and dissolve it with 2mL of distilled water; Dissolve M in PBS. Slowly drop liquid B into liquid A under ice-bath conditions, and stir with magnetic force at 4°C for 4 hours, then slowly add it into liquid C under ice-bath conditions, and adjust the pH to 8.0 after the addition is complete. The reaction was stirred overnight at room temperature with magnetic stirring. The final product was dialyzed against 0.01M PBS pH 7.4 to remove impurities to obtain the mabuterol conjugate.

Embodiment 2

[0046] Example 2 Preparation and Titer Detection of Whole Antigen Monoclonal Antibody

[0047] 2.1 Preparation of monoclonal antibodies

[0048] BSA-hapten was used as the immunogen to immunize 4 BALB / C mice, and 100 μg of the immunogen was taken from each mouse, mixed with an equal volume of Freund's complete adjuvant, emulsified evenly, and injected into the peritoneal membrane along the groin. Four weeks later, booster immunization was performed with the same dose and Freund's incomplete adjuvant as the adjuvant. After boosting the immunization three times, blood was collected to measure the titer. After the serum titer stopped rising, mice were immunized with twice the dose of antigen without adjuvant. Three days later, spleen cells and mouse myeloma cells were collected under sterile conditions and pressed for 5~ Mix in a 50mL centrifuge tube at a ratio of 10:1, add 30mL of serum-free IPMI1640 medium, centrifuge at 1200r / min for 10min, discard the supernatant, shake the ...

Embodiment 3

[0054] The preparation of colloidal gold test paper strip in the present invention in embodiment 3

[0055] 3.1 Preparation of colloidal gold

[0056] The basic principle of immune colloidal gold technology is that chloroauric acid can be polymerized into gold particles of a certain size under the action of a reducing agent to form a negatively charged hydrophobic colloid solution that is stable due to electrostatic interaction. Our company uses trisodium citrate as a reducing agent to prepare colloidal gold. The specific process is as follows: take 100mL aqueous solution of 0.01% chloroauric acid and heat it to boiling, and add 0.7mL 1% trisodium citrate aqueous solution accurately under stirring, and the golden yellow Chlorauric acid turns purple within 2 minutes, continue to boil for 15 minutes, and restore the original volume with distilled water after cooling, which is the prepared colloidal gold solution. Whether this colloidal gold solution meets the production require...

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Abstract

The invention discloses a mabuterol colloidal gold test strip and a preparation method thereof. The mabuterol colloidal gold test strip comprises a base plate, wherein the base plate is provided with a first end and a second end, and a sample cushion, a combiner cushion, a nitrocellulose membrane and a water sucking cushion are arranged on the base plate in sequence along the direction the first end to the second end. An anti-mabuterol monoclonal antibody which is marked by colloidal gold is contained on the combiner cushion. A detecting line and a quality control line are further formed on the nitrocellulose membrane, wherein the detecting line is composed of mabuterol detecting antigen threadiness sample application which can be combined with the anti-mabuterol monoclonal antibody. The quality control line is composed of ass anti-mouse antibody sample application which can be combined with the anti-mabuterol monoclonal antibody. The mabuterol colloidal gold test strip can be used for effectively detecting Mabuterol.

Description

technical field [0001] The invention belongs to the field of detection of immune colloidal gold and veterinary drug residues in the field of food safety. Specifically, the present invention relates to a mabuterol colloidal gold test strip and a preparation method thereof. Background technique [0002] β-agonist (β-agonist) full name β-adrenoceptor agonist (β-adrenoceptor agonist), also known as β-agonist, is a class of benzene with chemical structure and physiological function similar to epinephrine and norepinephrine. Ethylamines (phenethylamines, PEAs). It can selectively bind to the β-adrenergic receptors on the cell membrane to regulate sympathetic nerve excitation and relax tracheal smooth muscle. Therefore, it is often used clinically for the treatment of asthma-like diseases in humans and animals. However, when it is taken for a long time higher than the therapeutic dose, it can increase the lean meat rate of the raised animals, leading to an increase in the economi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N33/544G01N33/532C07K14/765C07K16/44
Inventor 赖卫华刘道峰邓省亮
Owner 江西中德生物工程股份有限公司
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