An immunomagnetic bead chromatography test strip and rapid detection method for detecting Cronobacter

Abstract

The invention relates to an immunomagnetic beads chromatography test paper strip for detecting cronobacter. A nitric acid fiber film of the test paper strip is provided with a detection line T and a control line C, the detection line T is prepared by a capture probe solution obtained by uniformly mixing a 100 [mu]M capture probe with a length being 20 bp, 5 mg / mL of streptavidin, and 50 mM of a phosphate buffer according to a volume ratio being 1:1:6; the control line C is prepared by a standard solution with concentration being 1-5 mg / mL which is obtained by uniformly mixing an anti-bovine serum albumin antibody solution and a phosphoric acid buffer. when the immunomagnetic beads chromatography test paper strip is used for detecting whether milk powder contains cronobacter or not, detected liquid is prepared by bacteria DNA extraction, polymerase chain reaction, and PCR product and immunomagnetic beads combination on detected milk powder; when the detected liquid is dropped on a sample pad of the immunomagnetic beads chromatography test paper strip, a reaction at the room temperature is carried out for 10 min to obtain the observation result. If the detection line T and the control line C display yellow lines, the detected liquid is positive; if the control line C displays yellow, the detected liquid is negative, and the detection reaction time of a practical sample is shortened to 8-11 h.

Description

Technical field

[0001] The invention belongs to the technical field of Cronobacterium gene detection, and specifically relates to an immunomagnetic bead chromatography test strip for rapid detection of Cronobacterium gene and a preparation method thereof. Background technique

[0002] Cronobacterium (also known as Enterobacter sakazakii) is a species of Enterobacteriaceae, which can cause severe neonatal meningitis, enterocolitis and bacteremia, and the mortality rate is as high as 50%. Cronobacterium has attracted the attention of relevant departments in many countries around the world. According to reports, foreign dairy giants have been recalled for this reason. After the US FDA in 2002 detected Cronobacterium in infant formulas produced by some international dairy giants in the country, another international dairy giant voluntarily recalled a batch of products produced in the United States that had detected very small amounts of Cronobacter in 2003. Special formula milk pow...

Images