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Cleaning agent with cronobacter spp. biofilm inhibiting effect and preparation method and application of cleaning agent

A Cronobacter and biofilm technology, applied in the field of biomedicine, can solve the problems of complicated preparation methods and frequent occurrence of bacterial drug resistance, and achieve the effects of simple preparation, reduced pollution and harm, and convenient use

Active Publication Date: 2019-03-12
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

CN20180208430 discloses a kind of chitosan oligosaccharide-antibiotic conjugate and its preparation method and application, mainly utilizes chitosan oligosaccharide-chloramphenicol antibiotics to inhibit Gram-positive bacterial biofilms, but the use of antibiotics will cause Bacterial drug resistance occurs frequently, and the pharmaceutical composition is mainly aimed at Gram-positive bacteria, but there is no report on Gram-negative bacteria, especially Cronobacter, and the preparation method of the composition is complicated
[0005] At present, there is no patent report on the effective inhibition method of Cronobacter biofilm

Method used

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  • Cleaning agent with cronobacter spp. biofilm inhibiting effect and preparation method and application of cleaning agent
  • Cleaning agent with cronobacter spp. biofilm inhibiting effect and preparation method and application of cleaning agent
  • Cleaning agent with cronobacter spp. biofilm inhibiting effect and preparation method and application of cleaning agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment 1: preparation of chitosan oligosaccharide mixture

[0041] Chitosan with a deacetylation degree of 95% was hydrolyzed with chitosanase to obtain a mixture, which contained 16.7% chitobiose, 11.7% chitotriose, 13.6% chitotetraose, 9.5% chitopentaose, and 8.3% chitosan %, chitosan 7.6%, chitosan 6.2%, chitosan 5.1%, chitosan 4.9%, chitosan 3.1%, chitosan 2.2%, chitosan 1.3%, chitosan 14 Sugar 1.2%, Pentadecanoose 1.6%, Hexadecanose 1.2%, Heptadecanose 1.3%, Octadecanose 1.8%, Nonadecanose 1.5%, Eicosan 1.2%, the above percentage content Both refer to quality scores.

Embodiment 2

[0042] Embodiment 2: preparation of chito-oligosaccharide mixture

[0043] Chitin is hydrolyzed with chitinase to obtain a mixture, which contains 12.8% chitobiose, 16.7% chitotriose, 8.6% chitinotetraose, 7.6% chitopentaose, and 10.3% chitohexaose , chitin heptaose 6.2%, chitooctaose 7.9%, chitin nine sugar 8.1%, chitin decaose 3.3%, chitin undecanose 1.8%, chitin decaose 2.3%, chitin thirteen sugar 2.8%, chitin tetradecanoose 2.5%, chitin pentadecose 2.1%, chitin hexadecanose 1.9%, chitin heptadecanose 1.2%, chitin octadecanoose 1.3%, chitin nonadecanose 1.5% , Chitoeicosan 1.1%, the above percentages refer to mass fractions.

Embodiment 3

[0044] Example 3: Biofilm formation after cleaning with different cleaning agents

[0045](1) In this example, the effects of the cleaning agent of the present invention and common cleaning agents on the apparent morphology of the biofilm were compared. The specific implementation is as follows: 15 parts of chitosan oligosaccharide mixture, 7 parts of sodium lauroyl sarcosinate, 8 parts of cocamidopropyl betaine, 6 parts of decyl glucoside, 4 parts of glycerin, 60 parts of water, and the heating temperature is 35°C , and stir to combine until clear. Set up control group 1 simultaneously: common commercially available feeding bottle dishwashing agent (domestic product, composition is: glycerin 4%, cocamidopropyl betaine 8%, decyl glucoside 6%, aloe extract 3%, all the other are water Control group 2: common commercially available baby bottle dishwashing agent (imported product, composition: glycerin 4%, cocamidopropyl betaine 8%, sodium lauroyl sarcosinate 7%, methylisothiazol...

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Abstract

The invention relates to a cleaning agent with a cronobacter spp. biofilm inhibiting effect and a preparation method and application of the cleaning agent. The cleaning agent is prepared from amino oligosaccharides or derivatives thereof or alginate oligosaccharides, an anionic surfactant, a zwitterionic surfactant, a nonionic surfactant, glycerin and water. The cleaning agent has a great cronobacter spp. biofilm inhibiting effect and is especially capable of effectively inhibiting bacterial adhesion in an early stage of film formation. Compared with a common cleaning agent on the market, thecleaning agent containing the amino oligosaccharides or derivatives thereof or alginate oligosaccharides, the anionic surfactant, the zwitterionic surfactant and the nonionic surfactant has the advantage that the biofilm inhibiting effect is remarkably improved.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a cleaning agent for inhibiting Cronobacter biofilm, a preparation method and application thereof. Background technique [0002] Cronobacter spp. is a non-spore-forming, motile, facultatively anaerobic, Gram-negative bacillus. It can cause infections in people of all ages, especially premature infants, infants and young children who are underweight or in poor health, and adults who are frequently treated with antibiotics, are immunocompromised, have a history of medical transplants, and the elderly. Cronobacter infection in infants can cause fatal necrotizing colitis, sepsis, and meningitis with severe neurologic sequelae. Adults infected with Cronobacter will have a series of symptoms such as conjunctivitis, biliary sepsis, urinary tract sepsis, and pneumonia. About 80 percent of bacterial infections in humans are caused by biofilms, according to the National I...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11D1/94C11D3/22C11D3/20C11D3/60B08B3/08C11D3/48
CPCC11D1/94C11D3/2065C11D3/221C11D3/222C11D3/48B08B3/08C11D1/662C11D1/90C11D1/10C11D2111/14
Inventor 赵黎明卢珺陈启明
Owner EAST CHINA UNIV OF SCI & TECH
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