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Cronobacter and application thereof

A technology of Cronobacter and bacterial agents, applied in the field of microorganisms, can solve the problems of agricultural product appearance and quality damage, and achieve the effect of efficiently degrading pesticide residues, enhancing activity, and simple cultivation methods

Active Publication Date: 2016-01-13
JIANGSU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The currently reported digestion methods can be roughly classified into non-biodegradation and biodegradation. Non-biodegradation includes physical methods, hydrolysis, photolysis, and biochemical degradation. These methods can only clean or remove pesticide residues on the surface of agricultural products, and may also Causes damage to the appearance and quality of agricultural products; and biodegradation includes phytoremediation degradation and microbial degradation. Compared with other methods, microbial degradation of pesticide residues is recognized as an effective, safe, cheap and non-secondary pollution method. Due to the remarkable advantages of pure ecological process, bioremediation technology has broad application prospects in the treatment of environmental pollution, especially soil pollution, and is the current focus of pesticide degradation research.

Method used

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  • Cronobacter and application thereof
  • Cronobacter and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 screening strains

[0031] 1. Obtaining the strain

[0032] (1) Sample collection:

[0033] In June 2013, the fresh plant samples of Asteraceae plant Asteraceae growing next to the waste liquid pool in the Jiangsu Academy of Agricultural Sciences were washed with tap water to clean the dust and soil on the surface of the plants, dried naturally, and surface disinfection: soaked in 75% alcohol for 3- 5min, then rinsed with sterile water 3-4 times, with 2.5% NaClO 2 Rinse for 2-5min, wash with sterile water 5 times;

[0034](2) Isolation and screening of bacterial strains: the roots, stems, and leaves of P. aeruginosa were collected for tissue grinding, the juice was absorbed and evenly spread on enriched medium plates, and cultured at 30° C. in the dark for 2-7 days.

[0035] (3) Strain enrichment culture: After the colony grows, pick a single colony and streak it on the inorganic salt medium (MSM) containing chlorpyrifos (the content of chlorpyrifos is 50mg...

Embodiment 2

[0043] Embodiment 2 prepares XFP-gy bacterial agent

[0044] 1. Strain activation: pick the Cronobacter XFP-gy obtained in Example 1 to the culture medium for strain preservation, streak at 30°C, pick a single colony for two times (24 hours each), and then pick A single colony is cultured in the strain activation medium at 30°C, 150-200rpm shaker for 12-24h;

[0045] 2. Preparation of liquid seeds: inoculate the bacteria species activated in step 1 according to the inoculum volume of 1% of the volume of the seed medium in the fermenter equipped with the seed medium, and cultivate in air at 25-38°C for 16-24h to obtain liquid seeds;

[0046] 3. Liquid fermentation: Inoculate liquid seeds into a fermenter equipped with seed medium, the inoculation amount is 1% of the volume of the medium, and culture at 30-35°C and 150rpm in the dark for 4-6 hours to obtain live bacteria Cultures;

[0047] 4. Bacterial agent preparation: Take 50ml of live bacterial culture and centrifuge at 4...

Embodiment 3X

[0048] Example 3 XFP-gy inoculant degrades chlorpyrifos under in vitro conditions

[0049] 1. Inoculate 2ml of the XFP-gy microbial agent obtained in Example 2 into 100ml of MSM medium, add chlorpyrifos to a final concentration of 20mg / L, and measure the initial OD of the medium 600 value and chlorpyrifos content; at the same time, the culture medium not inoculated with XFP-gy bacterial agent (adding the same volume of sterile saline as bacterial agent) was used as the control group, and each treatment was repeated 3 times;

[0050] Both the control group and the experimental group were cultured at 30°C and 200rpm in the dark, and samples were taken after 9 days to measure the OD 600 Value and chlorpyrifos residual amount, found that the residual amount of chlorpyrifos in the experimental group was 4.43mg / L on the 9th day, and the degradation rate reached 77.28%. The degradation rate is only 0.8%.

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Abstract

The invention discloses a cronobacter with the preservation number being CGMCC No. 11032. The cronobacter can be used for preparing XFP-gy bactericide for enhancing degradation of residual chlopyrifos in crops; compared with the prior art, the cronobacter can effectively degrade chlopyrifos residues in an environment, be fixed to crop roots for a long time, enhance the activity of the crops in degrading residual chlopyrifos and efficiently degrade pesticide residues at the premise that quality of the crops is not affected and is green and free of pollution; the cronobacter is extremely high in chlopyrifos degradation capability, simple in culture method, high in growth speed, not prone to variation and capable of being directly applied to degradation of chlopyrifos in soil and pesticide production waste water, crops such as paddy rice can be inoculated to serve as endophyte to be used for chlopyrifos residue control, and the cronobacter is suitable for being widely used and popularized.

Description

technical field [0001] The invention belongs to the field of microorganisms, in particular to a Cronobacter strain that can enhance the degradation of residual chlorpyrifos in crops ( Cronobacter sp. ) and its applications. Background technique [0002] Chlorpyrifos (chlorpyrifos) is an organophosphorus insecticide and acaricide with high efficiency, low toxicity, broad spectrum, low residue and low drug resistance developed by Dow Agrochemicals in 1965. The chemical name of the active ingredient is O,O-diethyl-O-(3,5,6-trichloro-2-pyridyl)phosphorothioate (O,O-diethyl-O-(3,5,6 -trichloro-2-pyridyl, phosphorothioate). Chlorpyrifos is stable at room temperature and in acidic medium, but it is easy to decompose in alkaline medium, difficult to dissolve in water, and easily soluble in most organic solvents. Since highly toxic organophosphorus pesticides are banned, As an effective substitute product, chlorpyrifos is used more and more in terms of dosage and scope of use. It is ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N25/32A01G7/06A01C1/00C12R1/01
Inventor 余向阳冯发运张猛葛静王琼
Owner JIANGSU ACAD OF AGRI SCI
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