68results about How to "Realize joint detection" patented technology

The invention provides a detection instrument and a detection method adopting the detection instrument. The detection instrument comprises a sample cell, an enrichment system, a detection system, a connecting sample cell, a conduit of the enrichment system and a driving device used for conveying a sample into the enrichment system. The detection instrument is characterized in that the enrichment system comprises a capillary pipe and a magnet, wherein the capillary pipe is a sample flow channel and an enrichment place; and the magnet is close to the capillary pipe and generates a magnetic field which is vertical to the flow direction of the sample; and the detection system is a fluorescent detection system. The detection method adopting the detection instrument can be used for detecting micro and trace amount of biological samples which comprise bacteria, virus or cell samples to be detected and small molecular semi-antigen samples to be detected.

The invention provides a measuring apparatus for the concentrations of nitric oxide and carbon monoxide in expired air. The measuring apparatus is composed of a sampling module and an analysis module. The sampling module is formed by tandem connection of a flow regulator, a stop valve and a sampling pump, wherein the flow regulator is connected with a flow/pressure sensor and a CO2 sensor, and a group consisting of an air chamber I, a solenoid valve I and a solenoid valve VI and a group consisting of an air chamber II, a solenoid valve II and a solenoid valve VII are arranged between the flow/pressure regulator and the sampling pump in a parallel connection manner, the solenoid valve I and the solenoid valve VI are connected with the air chamber I and respectively located before and behind the air chamber I, and the solenoid valve II and the solenoid valve VII are connected with the air chamber II and respectively located before and behind the air chamber II. A circulation air path is formed in the analysis module by the air chamber I, the solenoid valve I or the air chamber II, the solenoid valve II, a tee valve I, an analysis pump, a tee valve II, an air humidity regulator, a NO sensor, the solenoid valve VI or the solenoid valve VII; a normally open end of the tee valve I is connected with the front of the air chamber I or the air chamber II and with the analysis pump, and a passage switching end of the tee valve I is connected with the front of the air chamber I or the air chamber II and with a NO filter and the analysis pump; and a normally open end of the tee valve II is connected with the analysis pump, the air humidity regulator and the NO sensor, and a passage switching end of the tee valve II is connected with the analysis pump, a CO sensor and a CO filter.

The invention discloses a smart mobile terminal-based gas joint detection and alarming system, which is characterized by comprising three modules, including a multi-gas joint detection module, a data sampling and communication module and a mobile terminal application software module (based on android, iOS, Windows and the like), wherein the multi-gas joint detection module can detect various gases, convert gas concentration into electric signals, and output the electric signals; the data sampling and communication module can transmit sampled data to a smart mobile terminal; and the application software in the mobile terminal application software module analyzes the data from multiple sensors, and provides real-time character (voice, SMS (Short Messaging Service)) prompts (alarm) of different levels according to the user settings and the binding of special phone numbers. If a user further sets a cloud server address, the gas joint detection and alarming system can obtain the position information transmitted by a built-in GPS (Global Position System) of the smart mobile terminal and finish uploading of data, which is convenient for information statistics and information mining among different plant areas or regions, even different countries. The normal sensor has limited service life, the gas joint detection and alarming system can support replacement of sensors, based on the periodic upgrading of the software, the sensor of a new generation is supported by an updated database, and thus, the testing precision and the alarming accuracy are improved. Because of popularization of smart terminals and deterioration of some local environments, the gas joint detection and alarming system can be used for real-time monitoring and alarming for a personal environment so as to realize the aim of self protection.

The invention discloses a microfluidic chip as well as a preparation method and a detection method thereof. The microfluidic chip comprises a chip substrate and a chip cover plate, wherein the chip cover plate is provided with a sample adding opening for adding a detection sample; the chip substrate is provided with a reaction cavity located below the sample adding opening, a uniform mixing pipeline communicated with the reaction cavity, a detection pipeline communicated with the uniform mixing pipeline, and a waste liquid groove in sequence; a fluorescent microsphere labeling reagent for providing a fluorescent detection signal is arranged in the uniform mixing pipeline in a coating mode; a carrier film is loaded in the detection pipeline; the carrier film is coated with at least one capturing antibody reagent. The microfluidic chip disclosed by the invention can realize strict control of the re-dissolving volume and reaction time of a reagent and the detection repeatability is improved; the microfluidic chip is simple to package, the production technology of the chip is extremely simplified and the yield of the chip is improved; different regions in the carrier film can be coatedwith not less than one type of capturing antibody and multi-item combined detection is realized.

The invention discloses an SERS sensor for nucleic acid detection and a preparation and multielement detection method thereof. The sensor is composed of a solid SERS substrate and a nucleic acid probe chain. The nucleic acid probe chain is fixed to the surface of the solid SERS substrate through a gold-sulfur bond, after complementary base pairing with target nucleic acid molecules, sensing detection for nucleic acid is achieved by detecting the SERS signal change of Raman molecules on the probe chain, and exposed loci on the surface of the substrate are sealed through thiol hexyl alcohol. The solid SERS substrate is a silver nanorod array type substrate. The 3' end of the nucleic acid probe chain modifies thiol, the 5' end of the nucleic acid probe chain modifies dye molecules, part of base sequences in the probe chain are complementary to form a hairpin-shaped structure, and part of the base sequences and nucleic acid to be detected can be in complementary pairing. The detection limit of the sensor reaches the fmol level (fM), and joint detection of multiple nucleic acid markers can be achieved. The sensor is easy to prepare, high in detection sensitivity and good in reliability, and has wide application prospects in the fields of early tumor diagnosis and the like.

The invention relates to an authorization-free NOMA method for realizing URLLC based on a yoke transformation model, and the method comprises the following steps: achieving the estimation of the number of active terminals of an NOMA system based on a compressed sensing theory, and decoding a multi-terminal superposed signal; abstracting the interference pattern, solving the signal to interferenceplus noise ratio of the terminal in the transmission state, and solving the MAC-PHY joint reachable transmission rate according to the outage probability distribution; solving the time delay violationprobability of each terminal for a time delay and reliability joint analysis method meeting high reliability and low time delay for the multi-user uplink NOMA system based on a service yoke theory; and the optimal values of the terminal transmitting power and the access probability are solved by taking the maximum system energy efficiency as an optimization target, taking the delay reliability requirement as a constraint and taking the terminal transmitting power and the access probability as optimization variables. According to the invention, the collision problem of multi-user uplink unlicensed scheduling can be alleviated, the strict QoS guarantee of URLLC is realized, and the spectral efficiency and the system energy efficiency are improved.

The invention discloses a microfluidic chip for bedside diagnosis, a preparation method thereof and a detection method. The microfluidic chip for bedside diagnosis comprises a chip substrate and a chip cover plate, wherein a first sample introduction port for adding a detected sample and a second sample introduction port for adding a buffer solution are formed in the chip cover plate, a reaction tank communicating with the first sample introduction port, a buffer solution tank communicating with the second sample introduction port, a uniform mixing pipeline communicating with the reaction tank, a detection pipeline communicating with the buffer solution tank and the uniform mixing pipeline and a waste liquid tank communicating with the detection pipeline are arranged on the chip substrate,the interior of the uniform mixing pipeline is coated with a fluorescent microsphere tagging reagent for providing fluorescence detection signals, and the interior of the detection pipeline is coatedwith at least one antibody trapping reagent. According to the microfluidic chip for bedside diagnosis, disclosed by the invention, the repeatability of detection is high, a chip production process issimplified, multiple-item combined detection is achieved, and the sensitivity and specificity of detection are improved.

The invention discloses a fluorescence immunochromatography kit for simultaneously detecting CRP, lipoprotein phospholipase A2 and D-dimers and a preparation method thereof. The kit consists of an immunochromatography test paper strip and a plastic card case, wherein the immunochromatography test paper strip comprises a sample pad, a marker pad, a immunochromatography film, a water suction pad anda bottom plate; a fluorescent marker hs-CRP monoclonal antibody-1, a fluorescent marker Lp-PLA2 monoclonal antibody-1 and a fluorescent marker D-Dimer monoclonal antibody-1 are coated on the marker pad; hs-CRP monoclonal antibody-2, Lp-PLA2 monoclonal antibody-2 and D-Dimer monoclonal antibody-2 are respectively coated on a first detection line, a second detection line and a third detection lineon the immunochromatography film. According to the preparation method, the kit is obtained through fluorescent material processing, antibody marking, marker pad preparation, and immunochromatography film preparation and assembly. The kit realizes the combined detection of hs-CRP, Lp-PLA2 and D-Dimer; the detection and diagnosis efficiency is greatly improved; the structure of the kit is simple; the materials are popularized materials on the market; the industrial production cost can be greatly reduced.

The invention discloses a multichannel coherent detection method based on sliding window phase shift The method comprises steps: firstly building the mapping relation between a radar echo signal of each channel and the distance between spatial grids and a beam azimuth angle, and forming a retrieval information set of radar echo signal measurement of transmitting and receiving channels corresponding to the spatial grids; secondly extracting all echo signals, included in the spatial grids, of all channels according to a grid information-irradiation model retrieval matrix, carrying out the phase registration between the remaining channels and a receiving and transmitting channel 1 through employing the equal-step sliding window phase shift; finally carrying out the multichannel coherent detection of the spatial grids through employing the signals after registration, and traversing all spatial grids, thereby achieving the detection of a monitoring plane. The method makes the most of the valuable target echo phase information in multiple wave beams of all points after detection, and achieves the multichannel coherent joint detection. The method solves a problem that a conventional method cannot use the echo signal phase information, reduces the calculation amount, improves the signal to noise ratio after echo signal accumulation, and improves the detection performance of multichannel detection.

The invention discloses an FDA-MIMO radar incremental distance-angle two-dimensional beam forming method, which aims to realize accurate estimation of the distance of a target in each distance gate and improve the target detection performance. The method comprises the following steps of 1) acquiring a transmitting signal of an FDA-MIMO radar transmitting array element, 2) obtaining a receiving signal of a receiving array element after frequency mixing of a radar receiving end, 3) performing matched filtering on the transmitted waveform to obtain a sampling signal, 4) calculating a receiving signal vector after matched filtering of the radar receiving end, and 5) constructing a virtual steering vector according to the received signal vector, and finally obtaining an incremental distance-angle two-dimensional beam forming directional diagram. According to the method, the MIMO technology is adopted for the FDA array, joint beam forming is carried out at a receiving end, distance and angle decoupling is achieved, an antenna directional diagram dependent on the angle and the distance at the same time is generated, and incremental distance-angle joint detection and interference suppression of a target are achieved in a distance-angle joint domain.

The invention discloses a signal processing method of a wireless communication system, terminal equipment and network equipment, which are used for optimizing detection performance based on DFT-s-OFDMA. The signal processing method of the wireless communication system comprises the following steps: spreading a modulated signal, and carrying out discrete Fourier transform (DFT) processing on the spread signal; and mapping the signal after DFT processing to the input of discrete inverse Fourier transform expansion, performing inverse fast Fourier transform IFFT processing, and sending the processed signal to a receiving end.

The invention discloses a method for ovarian cancer screening based on an ovarian cancer marker and a logic gate operation, and belongs to the technical field of biomedicine. The protein-nucleic acidrecognition technology, the signal amplification technology and a logic gate are combined, aptamers of CEA and CA125 are used for target recognition, signal transformation and amplification are performed through a G-quadruplex-heme complex, and a method for simultaneous detection of tumor markers is established. According to the method, an optical biosensor with high sensitivity and strong specificity is constructed for achieving the joint detection of the CEA and the CA125, and an ovarian cancer screening result is provided based on the logic gate; through the combination of optical detectionand the signal amplification technology, the detection sensitivity is improved; the universality of the detection principle makes the method also suitable for detection of different objects based ondifferent protein recognition elements.

The invention discloses a Norovirus GI and GII type quantum dot joint inspection test strip as well as a preparation method and application of the Norovirus GI and GII type quantum dot joint inspection test strip. The test strip comprises a bottom plate, and a sample pad, a combination pad, a nitrocellulose membrane and a water absorption pad which are sequentially overlapped on the bottom plate from left to right, and the combination pad is coated with a quantum dot labeled Norovirus GI type detection antibody and a quantum dot labeled Norovirus GII type detection antibody. The test strip ofthe invention uses the quantum dots as markers, can be coupled with biomolecules easily, and is a novel rapid diagnosis and detection marker and has high fluorescence intensity and strong stability, so that the detection is higher in sensitivity, and the early screening of enterovirus infection and other diseases is convenient. The test strip is combined with an immunochromatography technology tobe prepared into a strip-shaped structure, is light and easy to carry, does not need the complex equipment, and is very suitable for the field screening and bedside detection.

The invention belongs to the technical field of medical biology. The invention discloses a test strip for screening early esophageal squamous cell carcinoma of high-risk groups. The test strip comprises a combination pad and a chromatography pad, the combination pad is coated with a capture antibody, the capture antibody is a mixture of a CFHR3 antibody A, a C1QTNF6 antibody A and an RFC2 antibodyA, and the CFHR3 antibody A, the C1QTNF6 antibody A and the RFC2 antibody A are all provided with detectable markers; three detection lines and a quality control line are arranged on the chromatography pad; detection antibodies fixed by the three detection lines are respectively a CFHR3 antibody B, a C1QTNF6 antibody B and an RFC2 antibody B; rabbit anti-mouse IgG is fixed on the quality controlline. The test strip provided by the invention can rapidly detect CFHR3, C1QTNF6 and RFC2 in human serum, realizes combined detection of three tumor markers, can effectively detect esophageal squamouscell carcinoma according to the detection result, and greatly improves the detection rate of early esophageal squamous cell carcinoma.

The invention belongs to the field of clinical medicine detection, and discloses a quality control product of an inflammatory marker and a preparation method of the quality control product. The quality control product of the inflammation marker is composed of a matrix liquid and an inflammation marker component, wherein the matrix liquid is composed of a matrix, a 5-100 mmol/L buffer agent, a 0.01-5 g/L protein protective agent, a 5-100 g/L saccharide and a 0.05-5 g/L preservative; the inflammation marker comprises the following components: procalcitonin, C reactive protein, serum amyloid protein A, myeloperoxidase, interleukin-6 and lipoprotein related phospholipase. The quality control product of the inflammatory marker contains a plurality of inflammatory markers, so that the joint detection of different inflammatory items is realized. Meanwhile, commercial human serum or plasma is adopted as a matrix, the channel source is stable, large-batch production can be achieved, the clinical sample conformity degree is high, the universality is high, the matrix effect can be reduced to the maximum extent, and the uniformity, stability and other performance are good.

The invention discloses a threshold-free detection method for a cloud platform, and the method comprises the steps: obtaining to-be-detected index data, and establishing models through a plurality ofabnormity detection algorithms; performing unsupervised abnormity detection on the to-be-detected index data according to the model; when any model obtains abnormal index data from the to-be-detectedindex data through unsupervised anomaly detection, judging whether a supervised model trained through corresponding historical index data exists according to the abnormal index data; when it is judgedthat no supervision model exists, all results of unsupervised abnormity detection are integrated to judge whether the to-be-detected index data are abnormal; when it is judged that the supervised model exists, performing supervised abnormity detection on the abnormal index data according to the supervised model, and judging whether the to-be-detected index data are abnormal according to a supervised abnormity detection result. The invention further discloses a corresponding device. According to the method, the joint detection method of the unsupervised model and the supervised model is realized, and the accuracy of the threshold-free detection result is improved.

The invention provides a crossroad intelligent traffic light network control system capable of handling an emergent event. The crossroad intelligent traffic light network control system includes a supersonic wave detection module, a microwave detection module, a control module and a traffic light module. By means of IoT technology, an IoT network operation system schedules and manages to form the detection equipment and the traffic light time of a traffic light IoT network system, so that the emergent event of the crossroad can be responded in real time, the combined detection and networking processing can be realized, and the aim of intelligent traffic light control can be achieved. Through a series of traffic management and service systems such as traffic signal control, travel induction, and bus information services, the rational distribution of traffic flow can be guided, the dynamic organization and management of urban traffic can be realized, the efficiency of traffic operation is improved, and the smooth and orderly city is guaranteed.

The invention discloses a rapid detection method of enterobacter sakazakii in dairy products. The rapid detection method comprises the following steps: firstly, extracting dairy product samples and extracting RNA (ribonucleic acid) of more dairy product samples; adding 1-5 micrograms of the RNA in step 1 into a 0.5ml microcentrifuge tube and supplementing DEPCH2O to enable the total volume to reach 11 mu l; adding 10 microns Oligo (dt)12-18 microliters into the tube; then uniformly shaking and centrifuging; after heating at 70 DEG C for 10 min, immediately inserting the microcentrifuge tube instep 2 into an ice bath for 1 min. According to the rapid detection method of the enterobacter sakazakii in the dairy products, disclosed by the invention, mRNA (messenger ribonucleic acid) is used as a target gene for detection and can be detected through methods including PCR (polymerase chain reaction) and the like; the mRNA can be rapidly degraded after bacteria are dead, so that the proper mRNA is selected as a target gene and only the bacteria with activity can be detected, and false positive interference caused by dead bacteria is avoided; and meanwhile, a PCR primer is improved and designed and a real-time fluorescent RT-PCR technology is applied, so that combined detection of various bacteria can be realized and the time and the cost are saved.

The invention relates to the technical field of biological detection, in particular to a feline herpes and feline calicivirus combined detection kit which comprises a detection card, a sample diluent and a sterile cotton swab, a test strip, a test strip nitrocellulose membrane, a water absorption pad, a gold-labeled antibody reaction pad and a sample pad are arranged in the detection card, a feline herpesvirus monoclonal antibody-colloidal gold compound coating and a feline calicivirus monoclonal antibody-colloidal gold compound coating are arranged on the gold-labeled antibody reaction pad, and the feline calicivirus monoclonal antibody-colloidal gold compound coating is positioned above the feline herpesvirus monoclonal antibody-colloidal gold compound coating; and an isolation pad is arranged between the feline calicivirus monoclonal antibody-colloidal gold compound coating and the feline herpes virus monoclonal antibody-colloidal gold compound coating. The kit is simple to operate and short in detection time, the detection efficiency is greatly improved, in addition, expensive instruments and equipment are not needed for detection, and the detection cost is reduced.

The invention belongs to the technical field of medical biology and discloses a test strip for esophageal precancerous lesion malignant progress risk detection or esophageal cancer early screening. The test strip comprises a combination pad and a chromatography pad, the combination pad is coated with a capture antibody, and the capture antibody is a mixture of a quantum dot labeled PSMC4 antibody,a quantum dot labeled ALDOC antibody and a quantum dot labeled TXLNA antibody; wherein three detection lines and a quality control line are sequentially arranged on the chromatographic pad along theflowing direction of a sample, and detection antibodies coated by the three detection lines are respectively a PSMC4 polyclonal antibody, an ALDOC polyclonal antibody and a TXLNA polyclonal antibody;and rabbit anti-mouse IgG is coated on the quality control line. The test strip provided by the invention can quickly detect PSMC4, ALDOC and TXLNA antigens in human serum, realizes combined detectionof three tumor markers, can predict the progression risk of esophageal precancerous lesion patients after 5 years according to the detection result, and is simple to operate and convenient to use.