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Test strip for screening early esophageal squamous cell carcinoma of high-risk groups

A technique for esophageal squamous cell carcinoma and test strips, which is applied in the field of medicine and biology, can solve problems such as rare application, achieve the effects of high sensitivity and specificity, reduce mortality, and improve detection rate

Active Publication Date: 2020-08-25
THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the application of multiple tumor marker combined detection methods and corresponding kits for the early diagnosis of esophageal squamous cell carcinoma is still relatively rare

Method used

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  • Test strip for screening early esophageal squamous cell carcinoma of high-risk groups
  • Test strip for screening early esophageal squamous cell carcinoma of high-risk groups

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Embodiment 1: the preparation of test strip

[0051] 1. Experimental materials

[0052] The bioactive raw materials used in the present invention are all commercially available products. Among them, CFHR3 monoclonal antibody was purchased from Shanghai Biological Yubo Biotechnology Co., Ltd., the article number is MAL329Hu21; CFHR3 polyclonal antibody was purchased from Shanghai Kemin Biotechnology Co., Ltd., article number K005454P; C1QTNF6 monoclonal antibody was purchased from Shanghai Qunji Biotechnology Co., Ltd. company, Cat. No. MAB16078; C1QTNF6 polyclonal antibody was purchased from Amigi Technology Co., Ltd., Cat. Aimeijie Technology Co., Ltd., catalog number H00005982-B01P.

[0053] PVC bottom plate, nitrocellulose membrane, glass fiber membrane, absorbent paper etc. are all existing commercially available products.

[0054] 2. Preparation of binding pads

[0055] (1) Preparation of colloidal gold:

[0056] Colloidal gold solution was prepared by trisodi...

Embodiment 2

[0080] Embodiment 2: the diagnostic value analysis of test strip

[0081] Use the test strip prepared in Example 1 of the present invention to detect the serum samples of patients with pathologically confirmed early esophageal squamous cell carcinoma and normal people, and evaluate and analyze the value of the test strip of the present invention for screening and diagnosis of early esophageal squamous cell carcinoma.

[0082] 1. Sample source

[0083] Serum samples were collected from the Henan Key Open Laboratory of Esophageal Squamous Cell Carcinoma in the First Affiliated Hospital of Zhengzhou University, including 80 samples of normal human serum (control group) and 80 serum samples of patients with early-stage esophageal squamous cell carcinoma (esophageal squamous cell carcinoma group). The sera of 80 normal people came from the healthy check-up population of the physical examination center of the laboratory's cooperative hospital, without any evidence of tumor-related. ...

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Abstract

The invention belongs to the technical field of medical biology. The invention discloses a test strip for screening early esophageal squamous cell carcinoma of high-risk groups. The test strip comprises a combination pad and a chromatography pad, the combination pad is coated with a capture antibody, the capture antibody is a mixture of a CFHR3 antibody A, a C1QTNF6 antibody A and an RFC2 antibodyA, and the CFHR3 antibody A, the C1QTNF6 antibody A and the RFC2 antibody A are all provided with detectable markers; three detection lines and a quality control line are arranged on the chromatography pad; detection antibodies fixed by the three detection lines are respectively a CFHR3 antibody B, a C1QTNF6 antibody B and an RFC2 antibody B; rabbit anti-mouse IgG is fixed on the quality controlline. The test strip provided by the invention can rapidly detect CFHR3, C1QTNF6 and RFC2 in human serum, realizes combined detection of three tumor markers, can effectively detect esophageal squamouscell carcinoma according to the detection result, and greatly improves the detection rate of early esophageal squamous cell carcinoma.

Description

technical field [0001] The invention belongs to the technical field of medicine and biology, and in particular relates to a test strip used for early screening of esophageal squamous cell carcinoma in high-risk groups. Background technique [0002] Esophageal squamous cell carcinoma is one of the six most common malignant tumors in the world, and its global morbidity and mortality rate ranks sixth. China is the country with the highest incidence and mortality rate of esophageal squamous cell carcinoma. The incidence rate occupies the fourth place, which is 100 times higher than that in western countries. Of the 500,000 new cases of esophageal squamous cell carcinoma in the world every year, more than half occur in China. The epidemiological characteristics of esophageal squamous cell carcinoma in China have significant regional distribution differences (the incidence rate difference between high and low incidence areas is 500 times, especially Linzhou, Henan Province and it...

Claims

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Application Information

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IPC IPC(8): G01N33/574G01N33/558G01N33/543
CPCG01N33/57407G01N33/54313G01N33/558
Inventor 王立东陈瑶宋昕赵学科王盼盼程锟王苒李贝韩雪娜范宗民常志伟
Owner THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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