Method for measuring nitrofuran antibiotics in cosmetics

A technology of nitrofuran and determination method, which is applied to measurement devices, instruments, scientific instruments and other directions, can solve the problems of auditory hallucinations, hallucinations, irritability, protracted and difficult to heal, etc., achieves accurate determination method, high specificity and sensitivity, avoids The effect of false positive results

Active Publication Date: 2014-07-16
CHINESE ACAD OF INSPECTION & QUARANTINE
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Problems solved by technology

[0003] Nitrofuran drugs are quickly absorbed after entering the human body, and their main toxic and side effects are as follows: (1) They have strong toxic effects on the human body, and long-term or high-dose exposure to nitrofuran drugs can produce toxic effects on the human body , of which nitrofurazone is the most toxic, and furazolidone is relatively less toxic; (2) carcinogenic, teratogenic and mutagenic side effects: furaltadone is a strong carcinogenic drug, a

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  • Method for measuring nitrofuran antibiotics in cosmetics
  • Method for measuring nitrofuran antibiotics in cosmetics
  • Method for measuring nitrofuran antibiotics in cosmetics

Examples

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Example Embodiment

[0035] Example 1

[0036] 1. Instruments and reagents

[0037] Agilent1200 liquid chromatograph (with diode array detector) (Agilent, USA); UV-Vis spectrophotometer (SHIMADZU UV-3600); Electronic balance (AB204-S, METTLER TOLEDO, USA); Ultra-pure water device (Milli- Q, American Millipore company); high-speed refrigerated centrifuge (CR21G, Japanese Hitachi company); MS2 type vortex oscillator (Germany IKA company); nitrogen blowing instrument (N-EVP112, American Organization Associates Inc); fume hood (FC- 150, Dalian Labtech Laboratory Equipment Co., Ltd.); Microporous membrane (0.22μm, organic, Pall, USA); methanol and acetonitrile are chromatographically pure (Fisher); ammonium acetate is analytically pure (content ≥98.0% , Xilong Chemical Co., Ltd., Shantou City, Guangdong Province)

[0038] The detailed information of nitrofurantoin, furazolidone, nitrofuran and nitrofurazone is shown in Table 4, and the structural formulas of the four nitrofuran antibiotics are shown in Tabl...

Example Embodiment

[0078] Example 2

[0079] Preparation and stability investigation of standard solution

[0080] During the preparation of the standard solution, according to the operating requirements in the literature, when preparing a 1mg / mL storage solution, it was found that it was difficult to completely dissolve the nitrofurazone standard with a general single organic solvent (acetonitrile and methanol). In the experiment, acetonitrile and methanol were used. The ratio between the mixed solution was used as a solvent to dissolve the substance, and it was found that when 20% methanol-acetonitrile was used, the compound could be completely dissolved.

[0081] Because nitrofurans themselves are unstable, they will be partially degraded and lost under conditions of high temperature, air exposure and direct sunlight. Therefore, this experiment investigated the chromatogram changes of four compounds under direct sunlight for 1h, 2h and 3h. ,Such as Figure 4 As shown, the high performance liquid ch...

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Abstract

The invention discloses a method for measuring nitrofuran antibiotics in cosmetics. The method comprises the following steps: (1) pretreating a sample, namely adding the sample into a mixed solution of acetonitrile and methanol in a volume ratio of 70:30, uniformly mixing, performing ultrasonic extraction, centrifuging, airing supernatant till the supernatant is nearly dry, dissolving residues in a mixed solution of a 15mmol/L ammonium acetate solution and acetonitrile according to a volume ratio of 85:15, and sieving the solution through a micro-porous filter membrane with the size of 0.22 micron, thereby obtaining filtrate for later use; (2) performing high performance liquid chromatography separation on the filtrate, eluting a mobile phase by a gradient elution program, and quantifying according to an external standard method; and (3) confirming a detected positive sample by high performance liquid chromatography-mass spectrometry/mass spectrometry. According to the method, four nitrofuran antibiotics such as macrodantin, furazolidone, furaltadone and nitrofurazone are simultaneously detected. The method is accurate, reliable and high in sensitivity.

Description

technical field [0001] The invention relates to a method for determining antibiotics, in particular to a method for determining nitrofuran antibiotics in cosmetics. Background technique [0002] Nitrofuran drugs are a kind of broad-spectrum antibiotics, which are widely used in clinic because of their strong bactericidal ability, broad antibacterial spectrum, resistance to drug resistance, low price and good curative effect. These antibiotics are mainly used in the treatment of urinary system infection, intestinal bacterial infection, skin wound infection and as a food additive to prevent poultry intestinal infectious diseases. The common nitrofuran antibiotics mainly include four kinds: nitrofurantoin (Furadantin, NFT), furazolidone (Furazolidone, FZD), furaltadone (Furaltadone, FTD) and nitrofuran (Furacilin, NFZ), which are all artificially synthesized with 5 - Antibacterial drug with nitrofuran basic structure. It is understood that although nitrofuran drugs will not c...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 马强孟宪双白桦陈云霞李晶瑞马会娟王元成
Owner CHINESE ACAD OF INSPECTION & QUARANTINE
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