Arsenic-free detection kit for iodide ions in trace serum sample

A technology of iodide ions and samples, applied in the field of detection, can solve the problems of easy contamination, easy decomposition, and large amount of serum, and achieve the effects of high safety, easy operation and high feasibility

Pending Publication Date: 2020-05-08
HARBIN MEDICAL UNIVERSITY
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Problems solved by technology

The existing serum iodine quantitative determination method has the disadvantages of complex reagent preparation, easy contamination, easy decomposition, large amount of dangerous reagents, and large amount of serum required

Method used

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  • Arsenic-free detection kit for iodide ions in trace serum sample
  • Arsenic-free detection kit for iodide ions in trace serum sample
  • Arsenic-free detection kit for iodide ions in trace serum sample

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Embodiment

[0049] 1. Principle: Use sodium chlorate-sulfuric acid solution to digest serum samples at 110°C, use iodine to catalyze the reduction reaction between sodium nitrite and ferric thiocyanate, and measure the concentration change rate of Fe(SCN)63- in relation to iodine content. Proportional; precise control of reaction temperature and reaction time, measurement of remaining Fe(SCN) 6 3- The absorbance, the iodine content has a linear relationship with the logarithm of the absorbance.

[0050] 2Fe(SCN) 6 3- +2I - →2Fe 2+ +12SCN - +I 2

[0051] I 2 +NO 2- +2OH - →2I - +NO 3 - +H 2 o

[0052] 2. Main instruments

[0053] 2.1 DTD8640 Blood Iodine Determination Digestion Apparatus (Temperature difference between holes ≤ 1°C, Jingjiang Aikerui Analytical Instrument Co., Ltd.), digestion hole diameter 8.3mm, 64 holes. Including 2 digestion tube holders.

[0054] 2. 28640 blood iodine determination digestion tubes (outer diameter 8mm x inner diameter 6mm x height 40mm...

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Abstract

The invention discloses an arsenic-free detection kit for iodide ions in a trace serum sample. According to the arsenic-free detection kit, the defect that a highly toxic reagent is used in the priorart is overcome, a sodium chlorate-sulfuric acid solution is used for digesting a serum sample at 110 DEG C, iodine is used for catalyzing the reduction reaction of sodium nitrite and ferric thiocyanate, and the concentration change speed of Fe(SCN)6<3-> is measured to be in direct proportion to the iodine content; the reaction temperature and the reaction time are precisely controlled, the absorbance of the remaining Fe(SCN)6<3-> is measured, and the iodine content and the logarithm of the absorbance are in a linear relation. An existing serum iodine quantitative determination method has thedefects of complex reagent preparation, easiness in pollution and decomposition, large dosage of dangerous reagents and large required serum amount. The arsenic-free serum iodine detection kit researched by the invention is convenient to operate, easy to carry, high in safety and suitable for clinically developing individual iodine nutrition detection.

Description

technical field [0001] The invention relates to a detection kit for iodide ions in trace serum samples, in particular to an arsenic-free detection kit for iodide ions in trace serum samples, belonging to the technical field of detection. Background technique [0002] Arsenic-cerium-catalyzed spectrophotometry is the health industry standard (WS / T572-2017), and is widely used in iodine deficiency laboratories at all levels in my country. Arsenic-cerium catalytic spectrophotometry has low equipment cost, manual operation and many operation steps. Due to the use of dynamic spectrophotometry, the cleanliness of the laboratory, the quality of the reagents, the digestion temperature, the reaction temperature, and the operating method all have an impact on the measurement results. There are many sources of error, and special training is required to master them. The reducing agent arsenic trioxide (commonly known as arsenic) used in the existing serum iodine detection method is hig...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N1/28
CPCG01N21/31G01N1/28
Inventor 刘颖卜野田春园纪春磊史梦琪李昕
Owner HARBIN MEDICAL UNIVERSITY
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