33results about How to "Do not interfere with determination" patented technology

The invention relates to a super fast liquid phase chromatography-tandem mass spectrometric method for determining hydroxypropyl-beta-cyclodextrin. The method comprises the following steps: (1) preparing hydroxypropyl-beta-cyclodextrin standard curve solution; (2) preparing hydroxypropyl-beta-cyclodextrin quality control solution; (3) treating a sample; and (4) performing liquid phase chromatography-tandem mass spectrometric analysis on the standard curve solution and the quality control solution respectively, and establishing a method for determining the hydroxypropyl-beta-cyclodextrin in the sample solution. The method has simple operation and good reproducibility, and can quantitatively analyze HP-beta-CD.

The invention relates to the detection of tripolycyanamide, in particular to a method and a device for detecting tripolycyanamide. The method comprises: preprocessing a sample to be detected to remove proteins; allowing obtained clear liquid to pass through an anionic and cationic ion exchange series column at a constant flow rate; transferring the clear liquid to be detected and buffer solution into a cell for samples to be detected; inserting an ion selective electrode modified by a molecular imprinted polymer into the cell; generating an electric potential signal; and determining the tripolycyanamide content of the sample to be detected by using an electric potential value according to a standard curve. In the device, one end of the anionic and cationic ion exchange series column, a second flow injection device and a container for the samples to be detected are connected with a three-way valve respectively; the other end of the anionic and cationic ion exchange series column is connected with an ultramicropore protein filter through a first flow injection device; and the second flow injection device is connected with a buffer solution tank; and the ion selective electrode modified by the molecular imprinted polymer is inserted into the container for the samples to be detected. The method and the device have the advantages of high flexibility, low operation cost, suitabilityfor in-situ monitoring and the like in the detection of tripolycyanamide.

The invention discloses a method for separating and detecting acetylcysteine ​​enantiomers. In an organic solvent, a derivatization reagent is used to carry out the separation and detection of N-acetyl-L-cysteine ​​and its enantiomers. The derivatization reaction is to prepare derivatized products of N-acetyl-L-cysteine ​​and its isomers, which are then separated and detected by normal phase high-performance liquid chromatography; the derivatization reagents are isocyanates. In the method for separating and detecting acetylcysteine ​​enantiomers of the present invention, the derivatization reagent is reacted with N-acetyl-L-cysteine ​​and its enantiomers, and pre-column derivatization is used for normal phase and high efficiency. Liquid chromatography, based on the mechanism of chiral separation, increases the mutual attraction between the stationary phase in the chromatographic column and the chiral substance to be separated, effectively improving the selectivity of chiral separation. The method is simple, time-consuming and easy to popularize and use.

A method for quickly and efficiently detecting trace p-phenylenediamine, specifically, dissolving cotton cellulose to prepare a regenerated cellulose film, using sodium periodate to selectively oxidize the hydroxyl groups on the regenerated cellulose film to aldehyde groups, and then pass The two-step Schiff base reaction prepares solid-phase fluorescence detection membranes of various systems emitting yellow fluorescence, which are used for qualitative analysis and quantitative detection of p-phenylenediamine. The invention has the advantages of simplicity, rapidity, high sensitivity and good selectivity.

The invention relates to a rapid simultaneous detection method for the content of copper, zinc and iron in gold mud. A sample is dissolved by utilizing nitric acid, aqua regia, sulfuric acid and nitric-sulfuric acid; after sulfuric acid is added for smoking, a copper-zinc-iron compound is decomposed to form a corresponding oxide, HAuCl4 is decomposed to form elemental gold, and H<n-1>[AgCln]<-n+1> is decomposed to form silver chloride; after sulfuric acid is added for dissolving salts, the copper iron zinc oxide is dissolved to form sulfate; after ammonia water is added, silver-copper-zinc is transformed into corresponding ammonia complex ions, iron is transformed into iron hydroxide precipitation, and gold and iron are filtered and then separated from filtrate; after the pH value of the filtrate is adjusted by adding sulfuric acid and ammonia water, sodium chloride is added for precipitation of silver, the silver is recovered by filtering, copper in the filtrate is detected by adopting iodometry, and zinc is detected by adopting an EDTA method; and iron is detected by using a potassium dichromate method. The sample consumption is less in a detection process, precious metals can be effectively classified and recovered, the detection process is simplified, the energy consumption is reduced, and the detection efficiency is improved.

The invention provides a coumarin derivative DOCOPA as well as a preparation method and an application thereof. The English name of DOCOPA is (E)-4-(3-(7-(diethylamino)-2-oxo-2Hchromen-3-yl)-3-oxoprop-1-en-1-yl) phenylacrylate). The preparation method comprises the steps that 3-acetyl-7-diethylaminocoumarin and p-hydroxybenzaldehyde are dissolved in acetonitrile firstly, a catalytic amount of piperidine is added, and reflux is performed until a reaction is performed sufficiently; an intermediate compound is further obtained; the intermediate compound and triethylamine are dissolved in dichloromethane, acryloyl chloride is added dropwise gradually to an ice bath, room-temperature stirring is performed until a reaction is performed sufficiently, a product is subjected to column chromatography separation after reduced-pressure evaporation, and the DOCOPA is obtained. The DOCOPA is applied to distinguishing and detection of cysteine and homocysteine in a pH7.8 system and imaging of the cysteine and the homocysteine in cells. The DOCOPA shows high sensitivity and selectivity for the cysteine and the homocysteine.

The present invention provides a coumarin derivative SS-590 and a preparation method and application thereof. The coumarin derivative SS-590, whose Chinese name is (E)-1,4-diethyl-8-( 3-(4-hydroxy-3-methoxyphenyl)acryloyl)-1,2,3,4-tetrahydro-7H-pyrano[2,3-g]quinoxaline-7-one, English Named (E)‑1,4‑diethyl‑8‑(3‑(4‑hydroxy‑3‑methoxyphenyl)acryloyl)‑1,2,3,4‑tetrahydro‑7H‑pyrano[2,3‑g]quinoxalin ‑7‑one, abbreviated as SS‑590. The invention provides a method for specific detection of human serum albumin, which is based on the coumarin derivative SS-590 to detect human serum albumin in PBS. The human serum albumin labeled with the coumarin derivative SS-590 in the present invention can be used for SDS-PAGE Gel analysis. It has broad application prospects in the field of biomolecular detection.

The invention discloses a method for detection of vanillin in food, and belongs to the field of analytical chemistry or the field of food safety. With a graphene-supported cuprous oxide nanoparticle composite film modified acetylene black electrode as a working electrode, the vanillin in the food is detected by an electrochemical method. The detection method has the advantages of wide linear range, high sensitivity, low detection limit, simple operation, fast detection, low detection cost and accurate results.

The present invention provides a kind of method based on HPLC-MS / MS technology detection NMDA receptor antagonist JCC-02 blood concentration, relates to the field of drug analysis, comprising the following steps: SD rats after JCC-02 intragastric administration In the blood plasma, add methanol, acetonitrile and internal standard working solution in sequence, vortex to take the supernatant and dissolve it with the mobile phase and then inject the sample. The internal standard working solution is the methanol solution of gliclazide; ‑The mixed solution of formic acid and water is used as the mobile phase for gradient elution, the Venusil ASB C8 chromatographic column is used for chromatographic separation, and the secondary mass spectrometer is used for detection and quantitative analysis. The method has strong specificity, high sensitivity, less sample volume, simple and rapid pretreatment, and short analysis cycle. The method is verified by methodology to be accurate and reliable, and is suitable for the determination of drug concentration and drug concentration of JCC‑02 in SD rat plasma. Kinetic studies.