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33results about How to "Do not interfere with determination" patented technology

Method and device for detecting tripolycyanamide

The invention relates to the detection of tripolycyanamide, in particular to a method and a device for detecting tripolycyanamide. The method comprises: preprocessing a sample to be detected to remove proteins; allowing obtained clear liquid to pass through an anionic and cationic ion exchange series column at a constant flow rate; transferring the clear liquid to be detected and buffer solution into a cell for samples to be detected; inserting an ion selective electrode modified by a molecular imprinted polymer into the cell; generating an electric potential signal; and determining the tripolycyanamide content of the sample to be detected by using an electric potential value according to a standard curve. In the device, one end of the anionic and cationic ion exchange series column, a second flow injection device and a container for the samples to be detected are connected with a three-way valve respectively; the other end of the anionic and cationic ion exchange series column is connected with an ultramicropore protein filter through a first flow injection device; and the second flow injection device is connected with a buffer solution tank; and the ion selective electrode modified by the molecular imprinted polymer is inserted into the container for the samples to be detected. The method and the device have the advantages of high flexibility, low operation cost, suitability for in-situ monitoring and the like in the detection of tripolycyanamide.
Owner:YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI

Method for detecting blood concentration of multiple antiepileptic drugs simultaneously

The invention belongs to field of medical examination and relates to assay determination method of internal medicine, specially a detecting method of six antiepileptics medicines of primidone, phenylethylmalonylurea, diphenylhydantoin, carbamazepine, Larmortriazine, okazepine and the active product monohydroxy okazepine of okazepine and the active product epoxy carbamazepine of carbamazepine in human plasma. The sample to be measured is eluted equicontinuously in condition of acidic mobile phase via the pretreatment of protein precipitation, and separated by chromatographic column, and detected by ultraviolet detector. The quantity of sample in the invention is small; the pretreatment method is simple, fast and sensitive, free of expensive apparatus and agents, applicability is wide, cost is low, it fits for the detection of normal blood concentration in clinic.
Owner:AFFILIATED HUSN HOSPITAL OF FUDAN UNIV

Separation and detection method of acetylcysteine enantiomer

ActiveCN109725073AIncrease mutual attractionHigh selectivityComponent separationChromatography columnChemistry
The invention discloses a separation and detection method of an acetylcysteine enantiomer. The method comprises the following steps: performing derivatization reaction in an organic solvent by using aderivatization reagent, N-acetyl-L-cysteine and an enantiomer thereof, so as to prepare a derivative product of N-acetyl-L-cysteine and isomers thereof; and performing separation and detection by using normal phase high-performance liquid chromatography, wherein the derivatization reagent is isocyanate. According to the separation and detection method of the acetylcysteine enantiomer, the derivatization reagent, N-acetyl L-cysteine and the enantiomer thereof are reacted, so that the mutual attraction effect between the stationary phase in the chromatographic column and the chiral substance tobe separated is increased according to the chiral separation mechanism by adopting precolumn derivatization normal phase high-performance liquid chromatography, and the chiral separation selectivityis effectively improved. The method is simple and convenient to operate, short in consumption time and easy to popularize and use.
Owner:HUNAN WARRANT PHARM TECH DEV CO LTD +1

Graphene-cuprous oxide composite film modified acetylene black electrode and detection method for detection of vanillin in food

The invention discloses a method for detection of vanillin in food, and belongs to the field of analytical chemistry or the field of food safety. With a graphene-supported cuprous oxide nanoparticle composite film modified acetylene black electrode as a working electrode, the vanillin in the food is detected by an electrochemical method. The detection method has the advantages of wide linear range, high sensitivity, low detection limit, simple operation, fast detection, low detection cost and accurate results.
Owner:HENGYANG NORMAL UNIV

Catalytic light mark and preparation method thereof, and method for determination of trace uranium by catalytic light mark

The invention discloses a catalytic light mark and a preparation method thereof, and a method for determination of trace uranium by the catalytic light mark. The catalytic light mark is composed of two parts of a DNA enzyme and a substrate DNA, a 3' end of a DNA enzyme sequence comprises four continuous guanine deoxynucleotides (G), the middle of a substrate DNA sequence has an adenine nucleotide (rA), and a 5' end of the substrate DNA is modified by a 4-methyl-6-carboxyl rhodamine (TAMRA) fluorescent dye. The DNA enzyme and the substrate DNA are in mixing hybridization to form the catalytic light mark, the catalytic light mark is added to a reaction system of a to-be-measured sample to undergo a reaction, the fluorescence intensity of uranyl nitrate ions with known concentration and the fluorescence intensity of the sample are determined, and the uranium content in the sample can be obtained through calculation. The method is a fast, accurate, high-sensitivity and high-selectivity method for determination of trace uranium.
Owner:EAST CHINA UNIV OF TECH

Method for detecting residual solvents in heparin sodium by headspace gas chromatography

InactiveCN108318615AOvercoming technical problems of poor applicabilitySuitability meets requirementsComponent separationRelative standard deviationPeak area
The invention relates to the technical field of solvent residual detection of drugs, and concretely relates to a method for detecting residual solvents in heparin sodium by headspace gas chromatography. The method optimizes the headspace sampling balance temperature which is an important parameter affecting the peak area ratio of the analytes to an internal standard by adopting the headspace gas chromatography against the technical problem of poor system adaptability of methods for detecting the residual solvents in the heparin sodium in the prior art, and the headspace balance temperature isoptimized to 78-82 DEG C from 90 DEG C. The method for detecting residual solvents in heparin sodium by headspace gas chromatography allows the relative standard deviation of the peak area ratio of the analytes to the internal standard during the repeated introduction of a control to be less than 1.0% and the system applicability to meet requirements, has the advantages of good specificity, good resolution of the analytes, good repeatability and high sensitivity, the detection limit of methanol is 0.0008%, the detection limit of ethanol is 0.0002%, the detection limit of acetone is 0.0001%, the quantitation limit of methanol is 0.0023%, the quantitation limit of ethanol is 0.0007%, and the quantitation limit of acetone is 0.0003%.
Owner:HUBEI YINUORUI BIOLOGICAL PHARMA

Coumarin derivative DOCOPA as well as preparation method and application thereof

The invention provides a coumarin derivative DOCOPA as well as a preparation method and an application thereof. The English name of DOCOPA is (E)-4-(3-(7-(diethylamino)-2-oxo-2Hchromen-3-yl)-3-oxoprop-1-en-1-yl) phenylacrylate). The preparation method comprises the steps that 3-acetyl-7-diethylaminocoumarin and p-hydroxybenzaldehyde are dissolved in acetonitrile firstly, a catalytic amount of piperidine is added, and reflux is performed until a reaction is performed sufficiently; an intermediate compound is further obtained; the intermediate compound and triethylamine are dissolved in dichloromethane, acryloyl chloride is added dropwise gradually to an ice bath, room-temperature stirring is performed until a reaction is performed sufficiently, a product is subjected to column chromatography separation after reduced-pressure evaporation, and the DOCOPA is obtained. The DOCOPA is applied to distinguishing and detection of cysteine and homocysteine in a pH7.8 system and imaging of the cysteine and the homocysteine in cells. The DOCOPA shows high sensitivity and selectivity for the cysteine and the homocysteine.
Owner:SHANXI UNIV

Cysteine detection method

The invention provides a method capable of detecting cysteine in a half-aqueous solution. Importantly, the method can distinguish cysteine from homocysteine. Based on a complex dichlorotetra[2-(2-pyridyl)phenyl]diiridium(III)[Ir2(ppy)4Cl2], in the half-aqueous solution of DMF (dimethylformamide) or DMSO (dimethylsulfoxide), the cysteine is directly detected by a fluorescent method. The determination process is simple and convenient and free from the interference of other amino acids including homocysteine and anions in the solution; and the method can be used for detecting whether cysteine exists or not.
Owner:SHANXI UNIV

Method for determining silicone oil in cosmetics

The invention discloses a method for determining silicone oil in cosmetics, which comprises the following steps that a test sample is dispersed with water in a color comparison tube; after being dispersed, the test sample is transferred to a separating funnel with water to be fully shaken; silicone oil in the test sample is extracted with normal hexane; after standing, moisture is removed through filtering, and a filtrate is obtained; and then the content of the silicone oil in the filtrate is determined by using the Fourier transform infrared spectroscopy. According to the method, the test sample matrix is dispersed with the water, the silicone oil is fully extracted with the normal hexane, the pretreatment is easy and quick, the dissolving capacity of the normal hexane to the silicone oil is strong, and the self infrared light does not interfere with the quantification of the silicone oil.
Owner:PONY TESTING INT GRP SHANGHAI CO LTD

Method for detecting content of heavy metals

The invention provides a method for detecting a content of heavy metals, which belongs to the technical field of the detection of metal ions. The method comprises the following steps: (1) preparing asolution to be detected: preparing a heavy metal sample to be detected into an aqueous solution, adding an acidic solution and a potassium iodide solution, and obtaining the solution to be detected; (2) detecting the solution to be detected: moving the solution to be detected, dropwise adding the solution to be detected onto test paper, developing the color for 50s to 100s, wherein the test papercontains dye; and (3) judging a detection result: visually comparing the color developing test paper and a colourimetric card, and determining a concentration of the heavy metal. By adopting the method for detecting the heavy metals, a rapid detection effect can be achieved, the detection efficiency is increased, and the field operation is also facilitated.
Owner:HEZHOU UNIV

Super fast liquid phase chromatography-tandem mass spectrometric method for determining hydroxypropyl-beta-cyclodextrin

The invention relates to a super fast liquid phase chromatography-tandem mass spectrometric method for determining hydroxypropyl-beta-cyclodextrin. The method comprises the following steps: (1) preparing hydroxypropyl-beta-cyclodextrin standard curve solution; (2) preparing hydroxypropyl-beta-cyclodextrin quality control solution; (3) treating a sample; and (4) performing liquid phase chromatography-tandem mass spectrometric analysis on the standard curve solution and the quality control solution respectively, and establishing a method for determining the hydroxypropyl-beta-cyclodextrin in thesample solution. The method has simple operation and good reproducibility, and can quantitatively analyze HP-beta-CD.
Owner:贾正平

Ultraviolet absorption measurement method for waste gas sulfur dioxide of stationary pollution source

The invention discloses an ultraviolet absorption measurement method for waste gas sulfur dioxide of a stationary pollution source. According to the method, an ultraviolet absorption method sulfur dioxide analyzer or multiple groups of gas analyzers with an ultraviolet absorption method sulfur dioxide analysis function is / are used as multiple monitors; sulfur dioxide is used for absorbing light with the characteristic wavelength of 240-330nm in a near ultraviolet light region, and the concentration of sulfur dioxide in waste gas is quantified according to the lambert-beer law; then the sulfur dioxide discharging rate is obtained by further calculation. An ultraviolet absorption method can adapt to wider measurement on SO2 discharged by the stationary source; by the method, the requirements on supervising monitoring on an on-site pollution source, comparison monitoring on a CEMS (continuous emission monitoring system) and checking of data effectiveness are met.
Owner:山东省环境监测中心站

Detection method of content of vitamin C in vinpocetine injection

The invention discloses a detection method of the content of vitamin C in a vinpocetine injection. The detection method comprises the following steps: carrying out spectrum scanning through an ultraviolet-visible spectrophotometer to determine the maximum absorption wavelength lambdamax of a vitamin C standard solution; then preparing one group of vitamin C solutions with different concentrations and determining a chromatographic peak area of each vitamin C solution through a high performance liquid chromatograph under the maximum absorption wavelength lambdamax; drawing a standard curve of the vitamin C solutions and the corresponding chromatographic peak areas and solving a linear regression equation; meanwhile, determining a chromatographic peak area of a test sample solution through the high performance liquid chromatograph under the maximum absorption wavelength lambdamax; and finally, calculating the content of vitamin C in the test sample solution. According to the detection method disclosed by the invention, the content of vitamin C in the vinpocetine injection is detected by adopting an HPLC (High Performance Liquid Chromatography) method; and the detection method is simple, convenient and feasible, has high specificity and high accuracy and provides guarantees for safety, effectiveness and controllability of drugs.
Owner:武汉华龙生物制药有限公司

Arsenic-free detection method of iodide ions in trace serum sample for individual iodine nutrition evaluation

The invention discloses an arsenic-free detection method of iodide ions in a trace serum sample for individual iodine nutrition evaluation. The arsenic-free detection method overcomes the defect thata highly toxic reagent is used in an existing method, adopts a sodium chlorate-sulfuric acid solution for digesting the serum sample at the temperature of 110 DEG C, uses iodine catalyzing the reduction reaction of sodium nitrite and ferric thiocyanate, and measures a concentration change speed of Fe(SCN)6<3-> to be in direct proportion to the iodine content; the reaction temperature and the reaction time are precisely controlled, the absorbance of the remaining Fe(SCN)6<3-> is measured, and the iodine content and the logarithm of the absorbance are in a linear relation. The establishment of the arsenic-free detection method can further meet the gradually increased demand of people for knowing the iodine nutrition level, a powerful method and means are added to serum iodine detection of iodine deficiency disease prevention and treatment monitoring and iodine nutrition evaluation, and the arsenic-free detection method has important public health practical significance and practical application value.
Owner:HARBIN MEDICAL UNIVERSITY

Arsenic-free detection kit for iodide ions in trace serum sample

The invention discloses an arsenic-free detection kit for iodide ions in a trace serum sample. According to the arsenic-free detection kit, the defect that a highly toxic reagent is used in the priorart is overcome, a sodium chlorate-sulfuric acid solution is used for digesting a serum sample at 110 DEG C, iodine is used for catalyzing the reduction reaction of sodium nitrite and ferric thiocyanate, and the concentration change speed of Fe(SCN)6<3-> is measured to be in direct proportion to the iodine content; the reaction temperature and the reaction time are precisely controlled, the absorbance of the remaining Fe(SCN)6<3-> is measured, and the iodine content and the logarithm of the absorbance are in a linear relation. An existing serum iodine quantitative determination method has thedefects of complex reagent preparation, easiness in pollution and decomposition, large dosage of dangerous reagents and large required serum amount. The arsenic-free serum iodine detection kit researched by the invention is convenient to operate, easy to carry, high in safety and suitable for clinically developing individual iodine nutrition detection.
Owner:HARBIN MEDICAL UNIVERSITY

Method for sensitively detecting tannin acid in beer

The present invention relates to a method for sensitively detecting tannin acid (TA) in beer. The method comprises the steps of: taking graphene oxide (GO) and ammonia water (NH3.H2O) as raw materials, and performing hydrothermal cutting under a strong alkaline condition to prepare amination GQDs (AF-GQDs). The polyphenolic hydroxyl structure of TA enables hydrogen bonding between the TA and the AF-GQDs, and a strong pi-pi interaction can be generated, so that fluorescence quenching of AF-GQDs is caused. A TA quantitative analysis method is established by using the AF-GQDs as a fluorescent probe, and the detection limit is 43.3 nmol / L<-1>; and the method has strong anti-interference ability, and detection of the TA is not interfered by the common small molecules, metal ions, anions, and the like.
Owner:QINGDAO UNIV

Super fast liquid phase chromatography-tandem mass spectrometric method for determining hydroxypropyl-beta-cyclodextrin

The invention relates to a super fast liquid phase chromatography-tandem mass spectrometric method for determining hydroxypropyl-beta-cyclodextrin. The method comprises the following steps: (1) preparing hydroxypropyl-beta-cyclodextrin standard curve solution; (2) preparing hydroxypropyl-beta-cyclodextrin quality control solution; (3) treating a sample; and (4) performing liquid phase chromatography-tandem mass spectrometric analysis on the standard curve solution and the quality control solution respectively, and establishing a method for determining the hydroxypropyl-beta-cyclodextrin in the sample solution. The method has simple operation and good reproducibility, and can quantitatively analyze HP-beta-CD.
Owner:贾正平

Method for determining antimony content in antimony oxide master batch by cerous sulfate titration method

The method comprises the following steps: (1) putting the antimony oxide master batch into a bottle, adding potassium sulfate and sulfuric acid, heating and dissolving on an electric furnace, taking down and slightly cooling, adding a reducing agent for reduction without shaking, and taking down and cooling when small bubbles in the solution disappear and thick white smoke is emitted to a bottle opening; (2) adding water along the bottle wall, uniformly shaking, heating and boiling, taking down and slightly cooling, adding hydrochloric acid, uniformly shaking, heating and taking down to prepare a heating solution; (3) adding an indicator into the heating liquid prepared in the step (2), uniformly shaking, keeping the temperature of the heating liquid, and dropwise adding a cerous sulfate standard titration solution until the red color of the solution just disappears, thereby obtaining an end point; and (4) calculating the mass fraction omega sb of antimony. The method is simple, reliable, convenient to operate and stable in result, can meet the requirement for analyzing the content of antimony in the antimony oxide master batch, and effectively solves the practical technical problem that the prior art is not suitable for detecting the total antimony in the antimony oxide master batch.
Owner:广西华锑科技有限公司

Method and device for detecting tripolycyanamide

The invention relates to the detection of tripolycyanamide, in particular to a method and a device for detecting tripolycyanamide. The method comprises: preprocessing a sample to be detected to remove proteins; allowing obtained clear liquid to pass through an anionic and cationic ion exchange series column at a constant flow rate; transferring the clear liquid to be detected and buffer solution into a cell for samples to be detected; inserting an ion selective electrode modified by a molecular imprinted polymer into the cell; generating an electric potential signal; and determining the tripolycyanamide content of the sample to be detected by using an electric potential value according to a standard curve. In the device, one end of the anionic and cationic ion exchange series column, a second flow injection device and a container for the samples to be detected are connected with a three-way valve respectively; the other end of the anionic and cationic ion exchange series column is connected with an ultramicropore protein filter through a first flow injection device; and the second flow injection device is connected with a buffer solution tank; and the ion selective electrode modified by the molecular imprinted polymer is inserted into the container for the samples to be detected. The method and the device have the advantages of high flexibility, low operation cost, suitabilityfor in-situ monitoring and the like in the detection of tripolycyanamide.
Owner:YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI

Method for determining concentration of chartreusin in blood plasma by liquid chromatography-tandem mass spectrometry

The invention discloses a method for determining the concentration of chartreusin in blood plasma through liquid chromatography-tandem mass spectrometry. The method comprises the following steps: separating chartreusin from a blood plasma sample by liquid chromatography, then establishing a standard curve by taking the concentration of chartreusin in a calibration standard sample as an X axis andthe peak area ratio of chartreusin to an internal standard substance in the calibration standard sample as a Y axis by utilizing a mass spectrum internal standard quantitative method, and calculatingthe concentration of chartreusin in the blood plasma. The method has the following advantages of: (1) strong specificity: the retention time of the chartreusin and the internal standard naringenin isabout 0.6min, and endogenous substances do not interfere with the determination of the chartreusin and the internal standard naringenin; (2) short analysis time: the detection time of each sample is 1min; (3) high sensitivity: the minimum quantification limit is 1ng.ML<-1>; and (4) the linear range of the blood plasma standard curve of the method is 1-1000ng.ML<-1>, and the intra-batch precision CV and the inter-batch precision CV are both less than 10%.
Owner:JIANGSU PROVINCIAL HOSPITAL OF TCM

A Rapid Joint Measurement Method of Copper, Zinc and Iron Content in Gold Mud

The invention relates to a rapid simultaneous detection method for the content of copper, zinc and iron in gold mud. A sample is dissolved by utilizing nitric acid, aqua regia, sulfuric acid and nitric-sulfuric acid; after sulfuric acid is added for smoking, a copper-zinc-iron compound is decomposed to form a corresponding oxide, HAuCl4 is decomposed to form elemental gold, and H<n-1>[AgCln]<-n+1> is decomposed to form silver chloride; after sulfuric acid is added for dissolving salts, the copper iron zinc oxide is dissolved to form sulfate; after ammonia water is added, silver-copper-zinc is transformed into corresponding ammonia complex ions, iron is transformed into iron hydroxide precipitation, and gold and iron are filtered and then separated from filtrate; after the pH value of the filtrate is adjusted by adding sulfuric acid and ammonia water, sodium chloride is added for precipitation of silver, the silver is recovered by filtering, copper in the filtrate is detected by adopting iodometry, and zinc is detected by adopting an EDTA method; and iron is detected by using a potassium dichromate method. The sample consumption is less in a detection process, precious metals can be effectively classified and recovered, the detection process is simplified, the energy consumption is reduced, and the detection efficiency is improved.
Owner:山东黄金冶炼有限公司

Ultraviolet Absorption Determination Method for Sulfur Dioxide in Stationary Pollution Source Exhaust Gas

The invention discloses an ultraviolet absorption measurement method for waste gas sulfur dioxide of a stationary pollution source. According to the method, an ultraviolet absorption method sulfur dioxide analyzer or multiple groups of gas analyzers with an ultraviolet absorption method sulfur dioxide analysis function is / are used as multiple monitors; sulfur dioxide is used for absorbing light with the characteristic wavelength of 240-330nm in a near ultraviolet light region, and the concentration of sulfur dioxide in waste gas is quantified according to the lambert-beer law; then the sulfur dioxide discharging rate is obtained by further calculation. An ultraviolet absorption method can adapt to wider measurement on SO2 discharged by the stationary source; by the method, the requirements on supervising monitoring on an on-site pollution source, comparison monitoring on a CEMS (continuous emission monitoring system) and checking of data effectiveness are met.
Owner:山东省环境监测中心站

Method for rapidly and efficiently detecting trace p-phenylenediamine

ActiveCN110530834AHigh optical clarityFluorescent background interference is smallFluorescence/phosphorescenceSolid phasesFluorescence
The invention discloses a method for rapidly and efficiently detecting trace p-phenylenediamine. The method specifically comprises the following steps of dissolving cotton cellulose to prepare a regenerated cellulose membrane; selectively oxidizing hydroxyls on the regenerated cellulose membrane into aldehyde groups by adopting sodium periodate; and carrying out a two-step Schiff base reaction toprepare a solid-phase fluorescence detection membrane of each system for emitting yellow fluorescence, wherein the solid-phase fluorescence detection membrane is used for qualitative analysis and quantitative detection of the phenylenediamine. The method has the advantages of simplicity and quickness, high sensitivity and good selectivity.
Owner:SOUTHWEST JIAOTONG UNIV

Quantitative analysis method for detecting blood concentration of YG-18 in rat plasma by liquid chromatography-mass spectrometry

The invention relates to a quantitative analysis method for detecting the blood concentration of rat plasma YG-18 by liquid chromatography-mass spectrometry, which belongs to the technical field of pharmacokinetics preclinical pharmacokinetics, and comprises the following steps: sequentially adding acetonitrile and an internal standard working solution into SD rat plasma after YG-18 administration, carrying out vortex centrifugation, taking supernatant, dissolving the supernatant with a mobile phase, and loading the sample, wherein an internal standard working solution is an acetonitrile solution of etophenanil; then carrying out quantitative analysis, wherein a liquid chromatography-mass spectrometry technology is adopted, an acetonitrile-formic acid solution is used as a mobile phase for gradient elution, and an Alltima C18 chromatographic column is used for the chromatographic separation. The method is strong in specificity, high in sensitivity, small in sample sampling amount, simple and rapid in pretreatment, short in analysis period, accurate and reliable through methodology verification, and suitable for YG-18 drug concentration determination and pharmacokinetic research in SD rat plasma.
Owner:DALIAN MEDICAL UNIVERSITY

A method for detecting plasma concentration of nmda receptor antagonist jcc-02 based on hplc-ms/ms technology

The present invention provides a kind of method based on HPLC-MS / MS technology detection NMDA receptor antagonist JCC-02 blood concentration, relates to the field of drug analysis, comprising the following steps: SD rats after JCC-02 intragastric administration In the blood plasma, add methanol, acetonitrile and internal standard working solution in sequence, vortex to take the supernatant and dissolve it with the mobile phase and then inject the sample. The internal standard working solution is the methanol solution of gliclazide; ‑The mixed solution of formic acid and water is used as the mobile phase for gradient elution, the Venusil ASB C8 chromatographic column is used for chromatographic separation, and the secondary mass spectrometer is used for detection and quantitative analysis. The method has strong specificity, high sensitivity, less sample volume, simple and rapid pretreatment, and short analysis cycle. The method is verified by methodology to be accurate and reliable, and is suitable for the determination of drug concentration and drug concentration of JCC‑02 in SD rat plasma. Kinetic studies.
Owner:THE FIRST HOSPITAL OF CHINA MEDICIAL UNIV
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