Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for Separation and Detection of Acetylcysteine ​​Enantiomers

A technology for the separation and detection of acetylcysteine ​​and enantiomers, which is applied in the field of separation and detection of acetylcysteine, can solve the problems of complex operation, low sensitivity, and unfavorable promotion and use, and achieve high sensitivity, strong specificity, Effects that are easy to promote

Active Publication Date: 2021-10-15
HUNAN WARRANT PHARM TECH DEV CO LTD +1
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The invention provides a method for separating and detecting acetylcysteine ​​enantiomers to solve the technical problems of complicated operation, low sensitivity and unfavorable popularization and use in the prior art

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for Separation and Detection of Acetylcysteine ​​Enantiomers
  • Method for Separation and Detection of Acetylcysteine ​​Enantiomers
  • Method for Separation and Detection of Acetylcysteine ​​Enantiomers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075] Sample to be tested 1: Accurately measure 1ml of acetylcysteine ​​solution for inhalation, the pH of the solution is neutral, put it in a 100ml measuring bottle, dilute with methanol to the mark, shake well, take 5ml precisely, put it in a 10ml measuring bottle ,stand-by;

[0076] Take another 25 mg of acetylcysteine ​​enantiomer reference substance, accurately weigh it, put it in a 25ml measuring bottle, add 3ml of 0.05mol / L sodium hydroxide methanol solution, dilute to the mark with methanol, shake well, and use it as a reference substance Mother liquor: Accurately measure 1ml of the mother liquor of the reference substance, put it in a 20ml measuring bottle, dilute to the mark with methanol, shake well, accurately measure 1ml, add it to the above-mentioned sample solution to be used, and then precisely add the derivative reagent (R)-( +)-a-methylbenzyl isocyanate 10μl, plugged tightly, shake well, put in 55°C water bath for 20min, let cool, dilute to the mark with me...

Embodiment 2

[0081] Sample to be tested 1: Accurately measure 1ml of acetylcysteine ​​solution for inhalation. The pH of the solution is neutral. in a bottle, ready to use;

[0082] Take another 25 mg of acetylcysteine ​​isomer reference substance, accurately weigh it, put it in a 25 ml measuring bottle, add 3 ml of 0.05 mol / L sodium hydroxide isopropanol solution, dilute to the mark with isopropanol, shake well, and use as Reference substance mother liquor: accurately measure 1ml of the reference substance mother liquor, put it in a 20ml measuring bottle, dilute to the mark with isopropanol, shake up, accurately measure 1ml, add it to the above-mentioned sample solution to be used, and then precisely add the derivative reagent ( S)-(-)-a-methylbenzyl isocyanate 10μl, plugged tightly, shake well, put in 50°C water bath for 18min, let cool, dilute to the mark with isopropanol, shake well, as a mixed solution;

[0083] Accurately measure 10 μl each of the reference substance solution and th...

Embodiment 3

[0086] Sample to be tested 2: Accurately measure 1ml of acetylcysteine ​​solution for inhalation. The pH of the solution is neutral. in a bottle, ready to use;

[0087] Take another 25 mg of acetylcysteine ​​isomer reference substance, accurately weigh it, put it in a 25 ml measuring bottle, add 3 ml of 0.05 mol / L sodium hydroxide isopropanol solution, dilute to the mark with isopropanol, shake well, and use as Reference substance mother liquor: accurately measure 1ml of the reference substance mother liquor, put it in a 20ml measuring bottle, dilute to the mark with isopropanol, shake up, accurately measure 1ml, add it to the above-mentioned sample solution to be used, and then precisely add the derivative reagent ( S)-(-)-a-methylbenzyl isocyanate 10μl, plugged tightly, shake well, put in 50°C water bath for 18min, let cool, dilute to the mark with isopropanol, shake well, as a mixed solution;

[0088] Accurately measure 10 μl each of the reference substance solution and th...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for separating and detecting acetylcysteine ​​enantiomers. In an organic solvent, a derivatization reagent is used to carry out the separation and detection of N-acetyl-L-cysteine ​​and its enantiomers. The derivatization reaction is to prepare derivatized products of N-acetyl-L-cysteine ​​and its isomers, which are then separated and detected by normal phase high-performance liquid chromatography; the derivatization reagents are isocyanates. In the method for separating and detecting acetylcysteine ​​enantiomers of the present invention, the derivatization reagent is reacted with N-acetyl-L-cysteine ​​and its enantiomers, and pre-column derivatization is used for normal phase and high efficiency. Liquid chromatography, based on the mechanism of chiral separation, increases the mutual attraction between the stationary phase in the chromatographic column and the chiral substance to be separated, effectively improving the selectivity of chiral separation. The method is simple, time-consuming and easy to popularize and use.

Description

technical field [0001] The invention relates to the field of separation and detection of acetylcysteine, in particular to a method for separation and detection of acetylcysteine ​​enantiomers. Background technique [0002] Acetylcysteine, chemical name N-acetyl-L-cysteine, molecular formula C 5 h 9 NO 3 S, is a respiratory mucus dissolving agent, which can decompose white mucus and thick sputum, so as to improve the patient's respiratory condition. Acetylcysteine ​​drugs have been approved for marketing at home and abroad, and are used to treat respiratory diseases with excessive thick mucous secretions, such as: acute bronchitis, chronic bronchitis and its deterioration, emphysema, and sticky substance obstruction syndrome and bronchiectasis. [0003] Acetylcysteine ​​contains a chiral center, and the chemical name of its enantiomer is N-acetyl-D-cysteine, which is an important indicator for the quality control of acetylcysteine. Patent 201010297844.9 discloses a metho...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 周志刚庞永清龙辉徐燕黄本东
Owner HUNAN WARRANT PHARM TECH DEV CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com