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Arsenic-free detection method of iodide ions in trace serum sample for individual iodine nutrition evaluation

A technology of iodine nutrition and iodide ion, applied in the field of detection

Pending Publication Date: 2020-05-08
HARBIN MEDICAL UNIVERSITY
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Problems solved by technology

[0003] The purpose of the present invention is to overcome the shortcomings of using highly toxic reagents in the existing methods, thereby providing an arsenic-free detection method for iodide ions in trace serum samples for individual iodine nutrition evaluation, using sodium chlorate-sulfuric acid solution at 110 ° C Serum samples were digested under certain conditions, and Fe(SCN) was measured by using iodine to catalyze the reduction reaction of sodium nitrite and ferric thiocyanate 6 3- The rate of change of the concentration of the iodine is proportional to the iodine content; precise control of the reaction temperature and reaction time, measurement of the remaining Fe(SCN) 6 3- The absorbance, the iodine content has a linear relationship with the logarithm of the absorbance

Method used

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  • Arsenic-free detection method of iodide ions in trace serum sample for individual iodine nutrition evaluation
  • Arsenic-free detection method of iodide ions in trace serum sample for individual iodine nutrition evaluation
  • Arsenic-free detection method of iodide ions in trace serum sample for individual iodine nutrition evaluation

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Embodiment

[0038] 1. Principle: use sodium chlorate-sulfuric acid solution to digest serum samples at 110 °C, and use iodine to catalyze the reduction reaction of sodium nitrite and ferric thiocyanate to measure Fe(SCN) 6 3- The rate of change of the concentration of Fe(SCN) is proportional to the iodine content; the reaction temperature and reaction time are precisely controlled to measure the remaining Fe(SCN) 6 3- The absorbance of , the iodine content has a linear relationship with the logarithm of the absorbance.

[0039] 2Fe(SCN) 6 3- +2I - →2Fe 2+ +12SCN - +I 2

[0040] I 2 +NO 2- +2OH - →2I - +NO 3 - +H 2 O

[0041] 2. Main instruments

[0042]2.1 DTD8640 blood iodine determination and digestion instrument (temperature difference between wells≤1℃, Jingjiang Aikerui Analytical Instrument Co., Ltd.), digestion aperture 8.3mm, 64 holes. Including 2 digestion tube racks.

[0043] 2.2 8640 blood iodine determination digestion tube (outer diameter 8mm×inner diameter ...

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Abstract

The invention discloses an arsenic-free detection method of iodide ions in a trace serum sample for individual iodine nutrition evaluation. The arsenic-free detection method overcomes the defect thata highly toxic reagent is used in an existing method, adopts a sodium chlorate-sulfuric acid solution for digesting the serum sample at the temperature of 110 DEG C, uses iodine catalyzing the reduction reaction of sodium nitrite and ferric thiocyanate, and measures a concentration change speed of Fe(SCN)6<3-> to be in direct proportion to the iodine content; the reaction temperature and the reaction time are precisely controlled, the absorbance of the remaining Fe(SCN)6<3-> is measured, and the iodine content and the logarithm of the absorbance are in a linear relation. The establishment of the arsenic-free detection method can further meet the gradually increased demand of people for knowing the iodine nutrition level, a powerful method and means are added to serum iodine detection of iodine deficiency disease prevention and treatment monitoring and iodine nutrition evaluation, and the arsenic-free detection method has important public health practical significance and practical application value.

Description

technical field [0001] The invention relates to a method for detecting iodine ions in trace serum samples used for individual iodine nutrition evaluation, in particular to an arsenic-free detection method for iodine ions in trace serum samples used for individual iodine nutrition evaluation, and belongs to the technical field of detection. Background technique [0002] The standard test method for serum iodine issued by my country "WS / T572-2017 Determination of Iodine in Serum - Arsenic-Cerium Catalytic Spectrophotometry", the method is acid digestion - Arsenic-Cerium catalytic spectrophotometry, the sample needs to be chlorinated before the determination. Sodium and concentrated perchloric acid digestion agent are pre-treated for 120min thermal digestion at 130°C. During the digestion process, there are a lot of free chlorine and other irritating gases escaping, and ventilation and detoxification equipment is required. The analysis speed is slow, and the photometric operation...

Claims

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Application Information

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IPC IPC(8): G01N21/31G01N1/28
CPCG01N21/31G01N1/28
Inventor 刘颖田春园卜野纪春磊史梦琪李昕
Owner HARBIN MEDICAL UNIVERSITY
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