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Quantitative analysis method for detecting blood concentration of YG-18 in rat plasma by liquid chromatography-mass spectrometry

A YG-18, technical detection technology, applied in the direction of measuring device, analytical material, material separation, etc., can solve the problem of no literature report, and achieve the effect of short analysis period, stable recovery rate and simple pretreatment

Pending Publication Date: 2021-12-31
DALIAN MEDICAL UNIVERSITY
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Problems solved by technology

It can be seen from the patent text that the chemical name of YG-18 is 3-[4-(4-bromobenzenesulfonyl)amino]benzoyl-2H-1-benzopyran-2-one, and the chemical structural formula is as follows figure 1 As shown, since YG-18 is an innovative drug, there is no literature report on the determination method of its drug concentration in biological samples at home and abroad. , it is necessary to establish a highly sensitive, convenient and quick method for quantitative analysis of YG-18 blood drug concentration

Method used

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  • Quantitative analysis method for detecting blood concentration of YG-18 in rat plasma by liquid chromatography-mass spectrometry
  • Quantitative analysis method for detecting blood concentration of YG-18 in rat plasma by liquid chromatography-mass spectrometry
  • Quantitative analysis method for detecting blood concentration of YG-18 in rat plasma by liquid chromatography-mass spectrometry

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Experimental program
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Embodiment Construction

[0042] Methodological research.

[0043] 1. Required instruments and reagents.

[0044] (1) Instruments.

[0045] Agilent 1260Infinity II high performance liquid chromatograph (Agilent Corporation, USA).

[0046] Agilent Ultivo triple quadrupole tandem mass spectrometer (American Agilent Company, Agilent Company, model Ultivo).

[0047] Constant temperature ultrasonic instrument (Shanghai Keqi Instrument Equipment Co., Ltd.).

[0048] XW-80A vortex mixer (Shanghai Qingpu Huxi Instrument Factory).

[0049] CPA124S electronic analytical balance (Germany Sartorius Scientific Instrument Co., Ltd., Sartorius company).

[0050] Scales (Cixi Tiandong Weighing Instrument Factory).

[0051] (2) Reagents.

[0052] YG-18 (purity, content ≥ 98.0%) (Department of Medicinal Chemistry, Shenyang Pharmaceutical University).

[0053] Ethoxyfensamide (purity content ≥99.9%, batch number: 200901) (National Institute for the Control of Pharmaceutical and Biological Products).

[0054] Acet...

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Abstract

The invention relates to a quantitative analysis method for detecting the blood concentration of rat plasma YG-18 by liquid chromatography-mass spectrometry, which belongs to the technical field of pharmacokinetics preclinical pharmacokinetics, and comprises the following steps: sequentially adding acetonitrile and an internal standard working solution into SD rat plasma after YG-18 administration, carrying out vortex centrifugation, taking supernatant, dissolving the supernatant with a mobile phase, and loading the sample, wherein an internal standard working solution is an acetonitrile solution of etophenanil; then carrying out quantitative analysis, wherein a liquid chromatography-mass spectrometry technology is adopted, an acetonitrile-formic acid solution is used as a mobile phase for gradient elution, and an Alltima C18 chromatographic column is used for the chromatographic separation. The method is strong in specificity, high in sensitivity, small in sample sampling amount, simple and rapid in pretreatment, short in analysis period, accurate and reliable through methodology verification, and suitable for YG-18 drug concentration determination and pharmacokinetic research in SD rat plasma.

Description

technical field [0001] The invention belongs to the technical field of preclinical pharmacokinetics of pharmaceutical analysis, in particular to a quantitative analysis method for detecting the plasma concentration of YG-18 in rat plasma by liquid chromatography-mass spectrometry (based on HPLC-MS / MS technology for detecting the concentration of YG-18 in SD rat plasma). Quantitative analysis method of blood drug concentration of novel α-glucosidase inhibitor YG-18), HPLC-MS / MS is high performance liquid chromatography-mass spectrometry / mass spectrometry. Background technique [0002] Traditionally, the development and research of α-glucosidase inhibitors are mostly based on their sugar-like skeleton structures, such as iminosugar, thiosugar or disaccharide structures. However, traditional research and development are faced with many problems, such as poor activity, low natural abundance and too complex stereochemistry. Therefore, it has broad prospects for the development o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/72
CPCG01N30/02G01N30/06G01N30/34G01N30/7266G01N2030/045
Inventor 王上阎成炟郭崇真张东虎
Owner DALIAN MEDICAL UNIVERSITY
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