Method for determining concentration of chartreusin in blood plasma by liquid chromatography-tandem mass spectrometry

A technique for teaching liquorice and liquid chromatography, which is applied in the field of liquid chromatography tandem mass spectrometry for determining the concentration of liquoricerin in plasma, and achieves the effects of high sensitivity, simple pretreatment method and short analysis time.

Active Publication Date: 2020-10-09
JIANGSU PROVINCIAL HOSPITAL OF TCM
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  • Description
  • Claims
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Problems solved by technology

[0004] At present, there is no literature report on the in vivo determination method of oxytocin

Method used

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  • Method for determining concentration of chartreusin in blood plasma by liquid chromatography-tandem mass spectrometry
  • Method for determining concentration of chartreusin in blood plasma by liquid chromatography-tandem mass spectrometry
  • Method for determining concentration of chartreusin in blood plasma by liquid chromatography-tandem mass spectrometry

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1: Determination of the concentration of oxytocin in rat plasma.

[0033] 1. Experimental materials and instruments

[0034] Teaching distillin: isolated from marine actinomycetes S. chartreusis NA02069; naringenin (internal standard): purchased from Chengdu Master Biological Technology Co., Ltd., batch number: MUST-19032406; test water: ultrapure water (produced in Millipore-QAdvantage A10 ultrapure water machine); methanol: chromatographically pure (Merck); acetonitrile: chromatographically pure (Merck); ammonium acetate: chromatographically pure (ACS).

[0035] API 4000LC-MS / MS coupled instrument (Applied Biosystems, USA), chromatographic workstation: Analyst 1.6; Mettler MS105 electronic balance (Mettler, Switzerland); MICRO 17R high-speed low-temperature centrifuge (Thermo, USA); Millipore -Q Advantage A10 ultrapure water machine (Millipore, France); VX-II multi-tube vortex oscillator (Beijing Tajin Technology Co., Ltd.).

[0036] 2. Liquid conditions

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Abstract

The invention discloses a method for determining the concentration of chartreusin in blood plasma through liquid chromatography-tandem mass spectrometry. The method comprises the following steps: separating chartreusin from a blood plasma sample by liquid chromatography, then establishing a standard curve by taking the concentration of chartreusin in a calibration standard sample as an X axis andthe peak area ratio of chartreusin to an internal standard substance in the calibration standard sample as a Y axis by utilizing a mass spectrum internal standard quantitative method, and calculatingthe concentration of chartreusin in the blood plasma. The method has the following advantages of: (1) strong specificity: the retention time of the chartreusin and the internal standard naringenin isabout 0.6min, and endogenous substances do not interfere with the determination of the chartreusin and the internal standard naringenin; (2) short analysis time: the detection time of each sample is 1min; (3) high sensitivity: the minimum quantification limit is 1ng.ML<-1>; and (4) the linear range of the blood plasma standard curve of the method is 1-1000ng.ML<-1>, and the intra-batch precision CV and the inter-batch precision CV are both less than 10%.

Description

technical field [0001] The invention belongs to the technical field of drug analysis, and relates to a method for analyzing and measuring drugs in vivo, in particular to a method for measuring the concentration of oxytocin in blood plasma by liquid chromatography tandem mass spectrometry. Background technique [0002] Chartreusin is a polycyclic aromatic polyketide compound originally isolated from Streptomyces chartreusis. Experiments in vivo and in vitro have proved that ditoxin has a strong anti-tumor effect. It can bind to DNA, break DNA through free radicals, and induce apoptosis of tumor cells. It is a drug source compound with good development prospects. Its structural formula is as follows: [0003] [0004] At present, there is no literature report on the in vivo determination method of oxytocin. The present invention establishes a liquid chromatography tandem mass spectrometry (LC-MS / MS) method with high sensitivity and high selectivity, which is used for dete...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/72G01N30/86
CPCG01N30/02G01N30/06G01N30/34G01N30/7266G01N30/8679G01N2030/045
Inventor 储继红戴国梁许美娟吴婷李长印欧阳冰琛居文政邹建东
Owner JIANGSU PROVINCIAL HOSPITAL OF TCM
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