Method and device for detecting tripolycyanamide

A technology of melamine and detection method, which is applied in the direction of measuring device, material analysis by electromagnetic means, instrument, etc., can solve the problems of not meeting the needs of melamine residue detection and high detection limit, and achieves easy automation, high sensitivity and accuracy, Simple and convenient operation

Active Publication Date: 2010-05-12
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The methods for detecting melamine developed in recent years include kit detection (ELISA), high performance liquid chromatography and gas chromatography, but the detec

Method used

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  • Method and device for detecting tripolycyanamide
  • Method and device for detecting tripolycyanamide
  • Method and device for detecting tripolycyanamide

Examples

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Example Embodiment

[0027] Example 1

[0028] Taking the present invention to test the content of melamine in a certain brand of pure milk as an example, there is no melamine in the milk at this stage. The determination steps are as follows:

[0029] a. Preparation of molecularly imprinted polymer: Take 352mg of melamine standard and 948μl of pre-vacuum-distilled α-methacrylic acid monomer into a 250ml round bottom flask, let it stand for 5min, and then add 6.3ml to the above mixed material Crosslinking agent ethylene glycol dimethacrylate and 123mg initiator azobisisobutyronitrile, ultrasonically oscillate for 10 minutes, after mixing uniformly, add 100ml of distilled benzene solvent to the above mixture, continue ultrasonic oscillation for 10 minutes, and finally Blow nitrogen for 10 minutes to remove oxygen in the reaction solution and seal. The above reaction vessel was moved to an oil bath and reacted at 75° C. for 17 hours to obtain white solid particles.

[0030] Grind the white solid particle...

Example Embodiment

[0038] Example 2

[0039] Take two different brands of milk and configure two spiked samples with a concentration of 5×10 -5 mol / l and 2.5×10 -5 mol / l, according to the steps d, f and g in Example 1 to determine the potential difference, refer to the standard working curve (see image 3 ), using multiple standard addition method to calculate the corresponding concentration, the measured recovery rate of standard addition is 110% and 95%.

Example Embodiment

[0040] Example 3

[0041] Take the determination of a certain brand of infant formula milk powder (for infants from 0-6 months) as an example.

[0042] a. Preparation of molecularly imprinted polymer: Take 352mg of melamine standard and 948μl of pre-vacuum distilled α-methacrylic acid monomer into a 250ml round bottom flask, let it stand for 5min. Then add 6.3ml of crosslinker ethylene glycol Dimethacrylate and 123mg initiator azobisisobutyronitrile were added to the above mixture, and ultrasonically oscillated for 10 minutes. After the mixture was uniform, 100ml of distilled chloroform solvent was added to the above mixture, and ultrasonic oscillation was continued for 10 minutes. Nitrogen for 10min to remove the oxygen present in the reaction solution and seal. Move the above reaction vessel to an oil bath and react at 60°C for 17h to obtain white solid particles.

[0043] The white solid particles were crushed and ground in an agate mortar and sieved. Take 500mg of 200-300 mesh ...

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Abstract

The invention relates to the detection of tripolycyanamide, in particular to a method and a device for detecting tripolycyanamide. The method comprises: preprocessing a sample to be detected to remove proteins; allowing obtained clear liquid to pass through an anionic and cationic ion exchange series column at a constant flow rate; transferring the clear liquid to be detected and buffer solution into a cell for samples to be detected; inserting an ion selective electrode modified by a molecular imprinted polymer into the cell; generating an electric potential signal; and determining the tripolycyanamide content of the sample to be detected by using an electric potential value according to a standard curve. In the device, one end of the anionic and cationic ion exchange series column, a second flow injection device and a container for the samples to be detected are connected with a three-way valve respectively; the other end of the anionic and cationic ion exchange series column is connected with an ultramicropore protein filter through a first flow injection device; and the second flow injection device is connected with a buffer solution tank; and the ion selective electrode modified by the molecular imprinted polymer is inserted into the container for the samples to be detected. The method and the device have the advantages of high flexibility, low operation cost, suitability for in-situ monitoring and the like in the detection of tripolycyanamide.

Description

technical field [0001] The invention relates to the detection of melamine, in particular to a detection method and device for the content of melamine in food. Background technique [0002] Melamine is an important nitrogen-heterocyclic organic chemical raw material and an important post-processing product of urea. The traditional detection methods of melamine include gravimetric method and sublimation method. The methods developed in recent years to detect melamine include kit detection method (ELISA), high performance liquid chromatography and gas chromatography, but the detection limit of these methods is high (≥5mg / Kg), or can only detect simple The matrix cannot meet the needs of detection of melamine residues in food. Contents of the invention [0003] The purpose of the present invention is to overcome the deficiencies of the existing chromatographic analysis technology, and provide a method and device for detecting melamine in food, especially raw milk and its pro...

Claims

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Application Information

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IPC IPC(8): G01N27/333G01N30/14
Inventor 秦伟梁荣宁张锐明
Owner YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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