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Extraction method and determination method for residual maleic hydrazide in tobacco

A determination method and extraction method technology, applied in the directions of measuring devices, instruments, scientific instruments, etc., can solve problems such as complicated tobacco extraction process, and achieve the effects of simple determination method, simple process and easy operation.

Active Publication Date: 2018-01-16
CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a method for extracting residual Yiyadan in tobacco, to solve the technical problem of complex extraction process of residual Yiyadan in tobacco in the prior art

Method used

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  • Extraction method and determination method for residual maleic hydrazide in tobacco
  • Extraction method and determination method for residual maleic hydrazide in tobacco
  • Extraction method and determination method for residual maleic hydrazide in tobacco

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] The extraction method of residual Yiyadan in the tobacco of the present embodiment comprises the following steps:

[0052] Weigh 2.00g (accurate to 0.01g) of smoke powder sample in a 100mL heat-resistant screw-necked Erlenmeyer flask, add 20mL 4mol / L hydrochloric acid aqueous solution to the bottle, add a magnetic stirrer and cover it, and follow the steps as follows: figure 1 Extract in the manner shown, specifically, place the Erlenmeyer flask in an oil bath placed on a magnetic heating stirrer, the temperature of the oil bath is 150°C, and extract with magnetic stirring at a speed of 300rpm for 1h. After cooling to room temperature, the extract After centrifugation at 10,000 rpm for 5 min, use C 18 Purify the supernatant with a solid-phase extraction cartridge, with a sample volume of 2 mL, elute with 2 mL of water, and filter the obtained purified solution through a 0.45 μm water phase filter membrane to obtain the final product.

[0053] The assay method of residu...

experiment example 1

[0071] Research on the influence of hydrochloric acid concentration on the extraction effect:

[0072] Use 1mol / L, 2mol / L, 3mol / L hydrochloric acid aqueous solution to extract tobacco dust sample respectively, other conditions are the same as embodiment 1, the result is as follows Figure 4 shown. Depend on Figure 4 It can be seen that with the increase of hydrochloric acid concentration, the extraction efficiency increases, and the final optimum concentration of hydrochloric acid is 4.0mol / L.

experiment example 2

[0074] Research on the influence of oil bath temperature on extraction effect:

[0075] The temperature of the oil bath was set at 60°C, 80°C, 100°C, and 120°C respectively to extract the smoke powder samples, and the other conditions were the same as in Example 1, and the results were as follows Figure 5 shown. Depend on Figure 5 It can be seen that with the increase of temperature, the extraction efficiency increases. Considering the tolerance of the Erlenmeyer flask, 150°C is finally selected as the optimal extraction temperature.

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Abstract

The invention relates to an extraction method and determination method for residual maleic hydrazide in tobacco, and belongs to the technical field of detection of pesticide residues in tobacco. The extraction method of residual maleic hydrazide in the tobacco includes the following steps: carrying out magnetic stirring and extraction of a tobacco sample and hydrochloric acid for 1-3 h under a condition of the temperature of 100-150 DEG C, then cooling to room temperature and centrifuging, then allowing the supernatant to pass through a column, purifying, and then eluting with water. The extraction method for residual maleic hydrazide in the tobacco has no need for reflux, has the advantages of simple and convenient operation, simple and fast process and high extraction efficiency, is suitable for extraction of residual maleic hydrazide in the tobacco samples, and has good consistency with a determination result of a national standard method.

Description

technical field [0001] The invention relates to an extraction method and a determination method of residual Yiyadan in tobacco, and belongs to the technical field of pesticide residue detection in tobacco. Background technique [0002] Yiyadan, also known as cyanin, maleic hydrazide, maleic hydrazide, is a plant growth regulator widely used in tobacco to inhibit the growth of axillary buds. Although there is no definite research result to prove that the use of Yiyadan can induce cancer, it has been confirmed that the use of large doses of Yiyadan can cause gene mutations. In the Guiding Residue Limits (GRLs) formulated by CORESTA and the enterprise standard of my country National Tobacco Corporation "YQ 50-2014 Maximum Residue Limits of Pesticides in Tobacco Leaf", the maximum residue limit of Yiyadan in tobacco is set at 80mg / kg. After Yiyadan is applied to tobacco, it can be absorbed into the plant through the cuticle of the leaf surface, part of which exists as a free st...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 邓惠敏边照阳杨飞孙莹莹刘珊珊唐纲岭范子彦李中皓王颖
Owner CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT
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