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Military micro-fluidic chip for detecting infection marker and detection method thereof

A microfluidic chip and marker technology, applied in fluid controllers, laboratory containers, chemical instruments and methods, etc., can solve the problems of difficult application, large chip size, complicated operation steps, etc., and achieve short detection time. , good mixing effect, simple operation effect

Active Publication Date: 2021-12-10
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In the microfluidic chip of the prior art, the movement steps of the fluid between the multi-layer chips are complicated, and only the traditional ELISA method is integrated, which does not reduce the detection time. In addition, the overall size of the chip is large, the operation steps are complicated, and the degree of specialization of the operation High, it is difficult to use as a POCT device, and it is difficult to apply to complex environments such as militarization

Method used

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  • Military micro-fluidic chip for detecting infection marker and detection method thereof
  • Military micro-fluidic chip for detecting infection marker and detection method thereof
  • Military micro-fluidic chip for detecting infection marker and detection method thereof

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Embodiment 1

[0058] refer to Figure 1 ~ Figure 4 , which is the first embodiment of the present invention, this embodiment provides a microfluidic chip for military detection of infection markers, which has a simple structure and is convenient for joint detection of multiple markers.

[0059] A microfluidic chip for military detection of infection markers, which includes a connection assembly 200, the connection assembly 200 includes an upper valve frame 202, the lower side of the upper valve frame 202 is connected with a lower valve frame 201, and the front and rear ends of the upper valve frame 202 are The first short pin 203 and the second short pin 204 connect the lower valve frame 201 together, there is an installation port between the upper valve frame 202 and the lower valve frame 201, and there are coaxial holes on the upper valve frame 202 and the lower valve frame 201 respectively. The upper connection hole 202a and the lower connection hole 201a of the heart, the microfluidic r...

Embodiment 2

[0068] For the second embodiment of the present invention, the difference from the first embodiment is that this embodiment provides a method for detecting using a military microfluidic chip for detecting infection markers, which includes the following steps:

[0069] Add the standard solution of the antigen or the diluted serum sample into the first liquid reservoir 102f;

[0070] Add CRP and PCT detection antibody coupled with HRP, IL-6 detection antibody coupled with B, and HRP coupled with SA into the second reservoir 102g;

[0071] Add PBST washing solution to the third liquid storage tank 102h;

[0072] Adding a chemiluminescence substrate into the fourth liquid reservoir 102i;

[0073] Press down the control valve 300 so that the two lower liquid outlet microchannels 301 communicate with the first liquid storage tank 102f and the second liquid storage tank 102g respectively, and the solutions in the first liquid storage tank and the second liquid storage tank are drawn...

Embodiment 3

[0079] The third embodiment of the present invention is different from the first and second embodiments in that this embodiment provides a method for assembling a microfluidic chip, which includes the following steps:

[0080] After cleaning the upper chip body 102 and the lower chip body 101, place them in a plasma cleaning machine to break the silicon-oxygen bond on the surface, place the detection layer 103 at a designated position between the two, press tightly, and avoid pressing the microchannel during the process After the upper chip body 102 and the lower chip body 101 are tightly bonded, the lower valve frame 201 is placed on the lower side of the lower chip body 101, and the first short pin 203 and the second short pin 204 are inserted to realize the upper valve frame 202 and the lower valve frame. The connection of the frame 201; finally insert the control valve 300, and the indicator key 303 is aimed at the entrance of the mixing microchannel 102l; the pictures of e...

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Abstract

The invention discloses a military micro-fluidic chip for detecting an infection marker and a detection method thereof. The military micro-fluidic chip comprises a connecting assembly, wherein the connecting assembly comprises an upper valve frame, the lower side of the upper valve frame is connected with a lower valve frame, the front end and the rear end of the upper valve frame are connected with the lower valve frame through a first short pin and a second short pin, an installation opening is formed between the upper valve frame and the lower valve frame, an upper connecting hole and a lower connecting hole which are coaxial are respectively formed in the upper valve frame and the lower valve frame, a micro-fluidic reaction assembly for detecting the infection marker is connected between the upper valve frame and the lower valve frame, and a control valve capable of moving in a height direction is connected to the micro-fluidic reaction assembly and the connecting assembly. Various solutions in the micro-fluidic reaction assembly are fed according to a detection sequence through the control valve. The military micro-fluidic chip is simple in structure, convenient to detect and short in detection time.

Description

technical field [0001] The invention relates to the technical field of detection of infection markers, in particular to a microfluidic chip for military use to detect infection markers and a detection method thereof. Background technique [0002] Point-of-care testing (POCT) can provide test results nearby and quickly, and provide guidance for diagnosis and treatment. Compared with traditional detection methods, POCT equipment has the characteristics of portability, easy operation, and rapid detection. For common diseases in the military, POCT equipment can provide a more convenient detection method, which can effectively guarantee the life, health and safety of soldiers in training grounds, battlefields and other complex militarized environments. [0003] Infection can range from minor skin trauma to severe organ failure, which is very common in military training, battlefield and other environments. Severe infection can lead to sepsis, and each hour of delay in sepsis tre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01L3/00G01N21/01G01N21/76
CPCB01L3/5027B01L3/502715B01L3/502738B01L3/50273G01N21/01G01N21/76B01L2300/0825B01L2200/10B01L2400/0463Y02A50/30
Inventor 尹彬沣万心华
Owner YANGZHOU UNIV
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