Microfluidic chip for bedside diagnosis, preparation method thereof and detection method

Abstract

The invention discloses a microfluidic chip for bedside diagnosis, a preparation method thereof and a detection method. The microfluidic chip for bedside diagnosis comprises a chip substrate and a chip cover plate, wherein a first sample introduction port for adding a detected sample and a second sample introduction port for adding a buffer solution are formed in the chip cover plate, a reaction tank communicating with the first sample introduction port, a buffer solution tank communicating with the second sample introduction port, a uniform mixing pipeline communicating with the reaction tank, a detection pipeline communicating with the buffer solution tank and the uniform mixing pipeline and a waste liquid tank communicating with the detection pipeline are arranged on the chip substrate,the interior of the uniform mixing pipeline is coated with a fluorescent microsphere tagging reagent for providing fluorescence detection signals, and the interior of the detection pipeline is coatedwith at least one antibody trapping reagent. According to the microfluidic chip for bedside diagnosis, disclosed by the invention, the repeatability of detection is high, a chip production process issimplified, multiple-item combined detection is achieved, and the sensitivity and specificity of detection are improved.

Description

technical field [0001] The invention belongs to the technical field of in vitro diagnosis of medical devices, and in particular relates to a microfluidic chip used for bedside diagnosis (POCT) and a preparation method and a detection method of the microfluidic chip. Background technique [0002] Point of Care Testing (POCT) refers to the on-site rapid diagnosis of patients with the help of miniaturized or moderate desktop equipment or reagents. Because the POCT detection method has the characteristics of simple and convenient operation, easy maintenance of the detection system, detection anytime and anywhere, and low detection cost, POCT detection products are gradually accepted and promoted by the market. [0003] POCT detection products used for disease diagnosis in the prior art can be roughly divided into two categories according to detection methodology or product materials: chromatography POCT detection technology and microfluidic POCT detection technology. [0004] C...

AI Technical Summary

Problems solved by technology

However, the active microfluidic chip POCT integrated with magnetic particle detection technology, because the magnetic particles coated with different antibodies cannot be separated by a magnetic field, the main defect of this detection technology is that it can only perform single-item detection. The control chip cannot perform multi-item detection at the same time

The active microfluidic chip POCT with integrated pressure valve needs to integrate an elastic film in the chip, which makes the entire chip packaging process extremely complicated, resulting in low chip manufacturing yield

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