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Detection instrument and detection method for biological sample

A detection instrument and sample technology, which is applied in the detection instrument and detection field for biological samples, can solve the problems of limited photosensitive area, expensive detection equipment, and limited resolution ability, and achieves the effect of huge application prospects and shortened time consumption

Active Publication Date: 2010-03-17
北京万德高科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The number of detection components using this technology is limited by the resolution capability of the detection device or scanning unit, as well as by the number of channels and photosensitive area, and the detection equipment is generally expensive
In addition, the above method has relatively low detection sensitivity when the sample to be tested is not amplified

Method used

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  • Detection instrument and detection method for biological sample
  • Detection instrument and detection method for biological sample
  • Detection instrument and detection method for biological sample

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0138] Take 10 mL of the sample to be tested containing Escherichia coli O157:H7 and immunomagnetic beads coupled with Escherichia coli monoclonal antibody (purchased at ) 200 μL and fluorescein isothiocyanate (FITC, purchased from Molecular )-labeled Escherichia coli-specific antibody 20 μL into the constant temperature sample pool 2, and incubated at 37°C for 10 minutes; switch the multi-position valve 8 to the inlet 9, switch the multi-position valve 20 to the outlet 22, and turn on the peristaltic pump 13 to drive the "immune magnetic beads -Analyte-fluorescent probe"formed complex solution flows through capillary 16, peristaltic pump 13, flow rate is set to 1mL / min, at the same time, start electromagnet 17 to enrich the magnetic beads suspended in the solution, and the residual liquid passes through The outlet 22 of the multi-position valve 20 flows out to the waste liquid bottle 24; after the magnetic beads in the liquid to be tested are enriched, the multi-position va...

Embodiment 2

[0140] Take 10 mL of the sample to be tested containing Escherichia coli O157:H7 and immunomagnetic beads coupled with Escherichia coli monoclonal antibody (purchased at ) 200 μL into the constant temperature sample pool 2, and incubate at 37°C for 10 minutes; switch the multi-position valve 8 to the inlet 9, switch the multi-position valve 20 to the outlet 22, and turn on the peristaltic pump 13 to drive the "immune magnetic beads - analyte" to form a compound The solution of the substance flows through the capillary 16, and the flow rate of the peristaltic pump 13 is set to 1mL / min. At the same time, the electromagnet 17 is started to enrich the suspended magnetic beads in the solution, and the raffinate flows out through the outlet 22 of the multi-position valve 20 to the waste liquid bottle. 24. After the magnetic beads in the solution to be tested are enriched, add 20 μL of FITC-labeled E. coli-specific antibody and 3 mL of PBS phosphate buffer into the constant temperatur...

Embodiment 3

[0142] Immunomagnetic beads (purchased at ) 200 μL, and quantum dots coupled with clenbuterol artificial antigen (emission wavelength 610nm, purchased at ) 100 μL into the constant temperature sample pool 2, and incubate at 25°C for 15 minutes; switch the multi-position valve 8 to the inlet 9, switch the multi-position valve 20 to the outlet 22, and turn on the peristaltic pump 13 to drive the "immune magnetic beads-fluorescent probe" to form a complex The solution flows through the capillary 16, and the flow rate of the peristaltic pump 13 is set to 1mL / min. At the same time, the electromagnet 17 is started to enrich the suspended magnetic beads in the solution, and the solution flows out to the waste liquid bottle 24 through the multi-position valve 20; The valve 8 is switched to the inlet 10, and the flow rate of the peristaltic pump 13 is set to 1 mL / min. At this time, the peristaltic pump 13 drives the buffer solution 14 to wash the enriched magnetic beads to remove the...

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Abstract

The invention provides a detection instrument and a detection method adopting the detection instrument. The detection instrument comprises a sample cell, an enrichment system, a detection system, a connecting sample cell, a conduit of the enrichment system and a driving device used for conveying a sample into the enrichment system. The detection instrument is characterized in that the enrichment system comprises a capillary pipe and a magnet, wherein the capillary pipe is a sample flow channel and an enrichment place; and the magnet is close to the capillary pipe and generates a magnetic field which is vertical to the flow direction of the sample; and the detection system is a fluorescent detection system. The detection method adopting the detection instrument can be used for detecting micro and trace amount of biological samples which comprise bacteria, virus or cell samples to be detected and small molecular semi-antigen samples to be detected.

Description

technical field [0001] The invention belongs to the field of analytical instruments, and specifically relates to detection instruments and detection methods for biological samples, and is especially suitable for detection instruments and detection methods for trace and trace biological samples, wherein immunomagnetic enrichment technology, immunofluorescence spectrum analysis technology and microfluidic The technology is integrated, and the fluorescence wavelength resolution method is used to realize the micro and trace analysis of single component and more than two components of biological samples. Background technique [0002] Micro and trace analysis of biological samples is of great significance in basic medicine, clinical medicine, and life science research, and has important application value in food science, environmental science, and even the fields of import and export inspection and quarantine. In the application of clinical medicine, food science and import and ex...

Claims

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Application Information

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IPC IPC(8): G01N33/53G01N21/64G01N1/40G01N33/569
Inventor 赵唯宇罗志勇高振宇
Owner 北京万德高科技发展有限公司
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