Test strip for joint detection of COVID-19 antigen and antibody and application thereof

A COVID-19, antigen-antibody technology, applied in the field of test strips for the joint detection of COVID-19 antigen-antibody, to avoid mutual interference, improve detection rate, and save cost and time

Pending Publication Date: 2020-08-14
HUNAN KANGRUN PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] None of the above patents have realized the detection of COVID-19 antigen and antibody on the same test paper

Method used

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  • Test strip for joint detection of COVID-19 antigen and antibody and application thereof
  • Test strip for joint detection of COVID-19 antigen and antibody and application thereof
  • Test strip for joint detection of COVID-19 antigen and antibody and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0043] Reagent test kit( figure 1 ) preparation:

[0044] 1. Preparation of sample diluent: the buffer used in the sample diluent is phosphate buffer pH 7.2, containing 0.1% Tween 20.

[0045] 2. Handling of the sample pad

[0046] ① The sample pad treatment solution contains 0.5% casein sodium salt, 0.4% Tween20, 3% sucrose, 0.3% erythrocyte ascites, contains 0.08% mouse IgG and rabbit IgG as blocking agents, and uses 20mM Tris-CL pH8. 8 in buffer solution.

[0047] ② Soak the glass cellulose membrane in the sample pad treatment solution and dry at 37°C for 20 hours.

[0048] 3. Preparation of colloidal gold bonding pad:

[0049] ① The buffer used in the binding pad treatment solution is an alkaline buffer pH8.5, such as borate buffer, containing 1% bovine serum albumin, 3% trehalose, and 0.4% surfactant TritonX -100, 0.3% Sodium Casein.

[0050] ② Soak the glass cellulose membrane in the bonding pad treatment solution and dry at 37°C for 20 hours.

[0051] ③Then mix t...

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Abstract

The invention relates to a test strip for joint detection of a COVID-19 antigen and an antibody and application of the test strip. The COVID-19 antigen and the antibody are detected at one time by adopting colloidal gold immunochromatography, and detection results of the COVID-19 antigen, IgM and IgG can be distinguished. Nasopharyngeal swab secreta and serum or plasma or whole blood or end tip blood are simultaneously subjected to sample adding; antigen-antibody combined detection is realized on a single test strip at one time; the 'window period 'of virus infection detection is prolonged; the cost and time are saved; the detection window time is advanced; early discovery and early isolation are realized; and the test strip has very important significance for screening potential infectious crowds.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and relates to a test strip for combined detection of COVID-19 antigen and antibody and an application thereof. Background technique [0002] The novel coronavirus (COVID-19) is seriously endangering human life and property in China and around the world. The disease generally has an incubation period of 3-7 days, and some patients have a longer incubation period. Patients have no obvious symptoms during the incubation period, but they are contagious. Once the disease develops, the fatality rate is high, so early screening and rapid diagnosis are of great significance. At present, the clinical diagnosis must be combined with nucleic acid diagnosis and CT scanning to achieve a confirmed diagnosis. However, the positive rate of nucleic acid diagnostic kits is low, and nucleic acid detection has high requirements for the experimental environment. General hospital laboratories and grassro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/58G01N33/577G01N33/569G01N33/558G01N33/531
CPCG01N33/6854G01N33/587G01N33/577G01N33/56983G01N33/558G01N33/531G01N2333/165G01N2469/10G01N2469/20
Inventor 胡道奇周松辉周咏武吴刚强童鹏李光
Owner HUNAN KANGRUN PHARMA
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