Alpha-thalassemia related gene detection kit

Abstract

The invention discloses an alpha-thalassemia related gene detection kit. The invention provides an alpha-thalassemia detection primer group for detecting alpha globin fusion gene, particularly primers with nucleotide sequences as shown in SEQ ID NO: 2-3, and a detection kit constructed based on the alpha-thalassemia detection primer group; and the operation is simple, the cost is low, the specificity is high, the result interpretation is visual, the popularization is easy, and the alpha-thalassemia detection primer group is suitable for being used in various occasions. Moreover, the primer group also has a very good multi-system general advantage, and can be matched with other detection primers for multiple PCR detection, for example, primers with nucleotide sequences as shown in SEQ ID NO: 4-5 or primers with nucleotide sequences as shown in SEQ ID NO: 6-7 can be used for detecting various types of alpha-thalassemia at one time. The technical scheme provided by the invention is of great significance to screening, genetic counseling and prenatal diagnosis of crowds suffering from thalassemia.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a detection kit for α-thalassemia-related genes. Background technique [0002] Thalassemia is an autosomal recessive genetic hemolytic disease caused by a defect in the globin gene, resulting in reduced or missing globin chain synthesis, resulting in an imbalance in the α-chain / non-α-chain ratio that forms hemoglobin. There may be no clinical manifestations, and severe cases are characterized by hemolytic anemia. According to the type of inhibition of globin peptide chain synthesis, thalassemia can be divided into α-thalassemia, β-thalassemia, δ-thalassemia, γ-thalassemia, δβ-thalassemia and εγδβ-thalassemia and so on. In addition, clinically, the disease is divided into three types: mild (thalassemia gene carrier), intermediate and severe. [0003] The α-globin gene is located in the α-globin gene cluster at the end of the short arm of human chromosome 16, which includes two repea...

AI Technical Summary

Problems solved by technology

[0007] In the traditional thalassemia screening process, if a person carries the fusion gene Fusion gene cannot be detected, if the person has the α 0 When a spouse with thalassemia gene gives birth to a child, the application of traditional detection kits may lead to wrong prenatal diagnosis results, resulting in the birth of children with HbH disease

[0008] At present, among the existing patented technologies of α-thalassemia detection products, only -- SEA 、-- THAI , -α 21.9 , -α 4.2 , -α 3.7 、α CS α, α QS α, α WS There is no direct detection of α globin fusion gene Fusiongene in the detection technology, method and kit of α, etc.

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