Bim (Bcl-2 interacting mediator of cell death) gene deletion fluorescent quantitative PCR (polymerase chain reaction) detection primer, probe and detection reagent kit

Abstract

The invention discloses a Bim (Bcl-2 interacting mediator of cell death) gene deletion fluorescent quantitative PCR (polymerase chain reaction) detection primer, a probe and a detection reagent kit, and belongs to the field of molecular biological detection. The Bim gene deletion fluorescent quantitative PCR detection primer and the probe comprise forward primers 5'-CAACAAACCCATCAGAACAGACAC-3', reverse primers 5'-ACAGCCTCTATGGAGAACAGTGATT-3 and fluorescent probes 5'-FAM-CAGACACTGGAACAAA-MGB-3'. The detection reagent kit comprises the Bim gene deletion fluorescent quantitative PCR detection primer, the probe, premixed liquid of PCR liquid and positive control samples A. The Bim gene deletion fluorescent quantitative PCR detection primer, the probe and the detection reagent kit have the advantages that Bim gene deletion mutation can be detected by the aid of the Bim gene deletion fluorescent quantitative PCR detection primer, the probe and the detection reagent kit, and the Bim gene deletion fluorescent quantitative PCR detection primer, the probe and the detection reagent kit are simple, convenient and feasible and are high in accuracy and sensitivity and particularly suitable for detecting clinical samples; Bim deletion mutation can be detected, and accordingly the Bim gene deletion fluorescent quantitative PCR detection primer, the probe and the detection reagent kit can bring convenience for guiding individual treatment on bodies.

Description

technical field [0001] The invention belongs to the field of molecular biology, and in particular relates to a fluorescent quantitative PCR detection primer, a probe and a detection kit for Bim gene deletion. Background technique [0002] At present, lung cancer has the highest mortality rate among malignant tumors in developed countries such as Europe and the United States, and its incidence rate is increasing year by year, of which NSCLC accounts for 80% to 85%. In recent years, significant progress has been made in the targeted therapy of lung cancer. Many types of targeted drugs with high efficiency and low toxicity have come out one after another and have been applied in clinical practice, mainly including targeted therapy targeting EGFR and targeting angiogenesis. Targeted therapy, matrix metalloproteinase inhibitor (TIMP), targeted therapy targeting farkinyl transferase, antisense oligonucleotide targeted therapy for lung cancer and targeted vaccine for lung cancer, e...

AI Technical Summary

Problems solved by technology

[0010] The present invention is based on the fluorescent quantitative PCR method. The real-time fluorescent quantitative PCR combines nucleic acid amplification, hybridization, spectral analysis and real-time detection technologies, and detects PCR products by means of fluorescent signals, which solves the problem that traditional PCR technology cannot quantify and amplifies product pollution. Problems, avoiding the pollution in the process of ordinary quantitative PCR operation, making it easy to operate, fast, and accurate results, has become a powerful tool for gene expression quantification, with high sensitivity and specificity, good repeatability and wide quantitative range, etc. features

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