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PRDX2 antibody colloidal gold detection test strip for rapidly screening patients with chronic obstructive pneumonia in situ

A chronic obstructive and detection test paper technology, applied in the field of biomedicine, can solve the problems of long time-consuming COPD screening, and achieve the effects of intuitive result interpretation, improved sensitivity, and low detection cost

Pending Publication Date: 2019-08-09
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the deficiencies in the prior art, the present invention provides a PRDX2 antibody colloidal gold detection test strip for on-site rapid screening of patients with chronic obstructive pneumonia, and improves the existing problem of long time-consuming early screening of COPD

Method used

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  • PRDX2 antibody colloidal gold detection test strip for rapidly screening patients with chronic obstructive pneumonia in situ
  • PRDX2 antibody colloidal gold detection test strip for rapidly screening patients with chronic obstructive pneumonia in situ

Examples

Experimental program
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Effect test

Embodiment 1

[0026] A PRDX2 antibody colloidal gold detection test strip for on-site rapid screening of patients with chronic obstructive pneumonia, the test strip includes a PVC bottom plate 1 and a sample pad 2, a gold standard pad 3, A chromatographic membrane 4 and a sample suction pad 5, the sample pad 2 includes an adsorption layer, a filter layer and a sample layer arranged sequentially from top to bottom; the adsorption layer is a porous solid material layer.

[0027] The sample suction pad 5 includes absorbent paper, and the lower surface of the absorbent paper is provided with a porous solid material layer. The filter layer is qualitative filter paper.

[0028] The chromatographic membrane 4 is a nitrocellulose membrane; a detection line T line 6 and a quality control line C line 7 are arranged on the nitrocellulose membrane.

Embodiment 2

[0030] The experimental methods without specific conditions indicated in the following examples are generally carried out according to conventional conditions, such as the conditions described in "Molecular Cloning: A Laboratory Guide" 4th edition.

[0031] Present embodiment is to provide the preparation method of test strip 4 described in embodiment 1, and the steps are as follows:

[0032] A method for preparing PRDX2 antibody colloidal gold detection test strips for on-site rapid screening of patients with chronic obstructive pneumonia,

[0033] 1. Anti-PRDX2 antibody and mouse IgG-labeled colloidal gold particles

[0034] Prepare colloidal gold by trisodium citrate reduction method: add 200ml of deionized water to the round bottom flask treated with soaking acid and silicification, heat it to boil with electric heating mantle, stir at 200r / min; quickly add 2ml of 1% chlorine Bring the gold acid solution to boiling; add 6ml of trisodium citrate solution, the solution will...

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Abstract

The invention relates to a PRDX2 antibody colloidal gold detection test strip for rapidly screening patients with chronic obstructive pneumonia in situ. The test strip PVC comprises a PVC bottom plateas well as a sample pad, a gold labeled pad, a chromatographic membrane and a sample absorption pad which are sequentially in lap joint with the PVC bottom plate; the sample pad comprises an adsorption layer, a filtering layer and a sample layer; the sample absorption pad comprises absorbent paper; and the lower surface of the absorbent paper is provided with a porous solid material layer. The PRDX2 antibody colloidal gold detection test strip for rapidly screening patients with chronic obstructive pneumonia in situ of the invention has the advantages of strong specificity and high sensitivity. According to the PRDX2 antibody colloidal gold detection test strip of the invention, widely applied and high-efficiency antigen-antibody binding reactions are adopted to perform detection, and therefore, the level of proteins in a blood sample can be accurately detected; and a high-quality monoclonal antigen and an antibody are combined so as to realize capture, so that the sensitivity of thenucleic acid detection test strip is greatly improved.

Description

technical field [0001] The invention relates to a PRDX2 antibody colloidal gold detection test strip for rapid on-site screening of patients with chronic obstructive pneumonia, belonging to the field of biomedicine. Background technique [0002] COPD is a preventable and treatable disease characterized by airflow limitation that is not fully reversible, progressive and associated with an abnormal inflammatory response of the lungs to noxious gases or particles such as cigarette smoke. COPD mainly involves the lungs, but it can also cause systemic (or extrapulmonary) adverse effects. [0003] Pulmonary function tests are important in identifying airflow limitation. After inhalation of a bronchodilator, the forced expiratory volume in one second (FEV 1 ) / forced vital capacity (FVC) <70% indicates airflow limitation that cannot be fully reversed. Chronic cough and sputum production often precede airflow limitation for many years; however, not all patients with cough and s...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/558G01N33/52
CPCG01N33/52G01N33/558G01N33/6884G01N33/6893
Inventor 陈瑞刘宏孙浩李晓波
Owner SOUTHEAST UNIV
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