Production method of growth factor supported porous biologic ceramic artificial bone scaffold

Abstract

The invention discloses a production method of a growth factor supported porous biologic ceramic artificial bone scaffold, and belongs to the field of medical science and biomedical engineering. Qualitative and quantitative detection of a cobra venom nerve growth factor (NFG) adsorbed through a biphasic calcium phosphate ceramic (BCP) artificial bone scaffold and artificial bone scaffold function detection prove that the NGF supported BCP is in favor of realizing osteoblast proliferation and directed differentiation. The NGP supported BCP has a good ossification promotion effect, provides a possible method for producing osteoblast in vitro culture scaffolds, and makes researches of bioengineering materials be possible.

Description

technical field [0001] The invention belongs to the fields of medicine and biomedical engineering, and in particular relates to a method for preparing a porous bioceramic artificial bone support loaded with growth factors. Background technique [0002] Large bone defects are a difficult problem. Autologous bone transplantation, which used to be the gold standard, due to limited materials, postoperative subcutaneous hematoma, infection, pain, poor appearance, paresthesia and other complications (JakoiAM et al., 2015, MusculoskeletSurg99:171-178), researchers gradually turned their attention to The study of bioengineering materials comes up. Bioengineering materials, for bone biomimetic materials, can also be called artificial bone scaffold materials, which mainly play the role of local filling and directional induction of osteogenesis in bone defects. From the early research on natural materials such as allogeneic bone, collagen, coral, etc. to later synthetic materials suc...

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Problems solved by technology

[0003] Bioactive factors are widely used in bone repair, which can be used to promote the wound repair of bone tissue and promote the proliferation, migration and differentiation of bone cells; however, currently commonly used growth factors, such as bone morphogenetic proteins (BMPs) exist to extract The process is complex, expensive, easy to degrade, and many other disadvantages. At the same time, it has been reported that it has side effects such as bone resorption and heterotopic ossification (BenglisD et al., 2008, Neurosurgery62: ONS423-ONS431), and nerve growth factor (nervegrowthfactor, NGF), there are Studies have confirmed that it has positive effects on the nervous system, cardiovascular system, immune system-related diseases, and even skin and bone repair (AloeL et al., 2012, JTranslMed10:239), which is expected to avoid the above-mentioned side effects and promote osteogenesis more safely and effectively However, studies have found that different species, different subspecies or even different sources of NGF extracted by the same process may have different activities, immunogenicity, and functions, and the activity and stability of NGF derived from snake venom may be better (LippsBV et al., 2000, JNat Toxins 9:13-19)

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