Production method of growth factor supported porous biologic ceramic artificial bone scaffold

A bioceramic and growth factor technology, applied in the field of medicine and biomedical engineering, can solve the problems of easy degradation, complex extraction process, heterotopic ossification, etc.

Inactive Publication Date: 2016-05-11
GUANGXI MEDICAL UNIVERSITY
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Problems solved by technology

[0003] Bioactive factors are widely used in bone repair, which can be used to promote the wound repair of bone tissue and promote the proliferation, migration and differentiation of bone cells; however, currently commonly used growth factors, such as bone morphogenetic proteins (BMPs) exist to extract The process is complex, expensive, easy to degrade, and many other disadvantages. At the same time, it has been reported that it has side effects such as bone resorption and heterotopic ossification (BenglisD et al., 2008, Neurosurgery62: ONS423-ONS431), and nerve growth factor (nervegrowthfactor, NGF), there are Studie

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  • Production method of growth factor supported porous biologic ceramic artificial bone scaffold
  • Production method of growth factor supported porous biologic ceramic artificial bone scaffold
  • Production method of growth factor supported porous biologic ceramic artificial bone scaffold

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[0036] , BCP adsorption NGF qualitative detection

[0037] The BCP immersed in 0μg / ml NGF solution (control group) and 10μg / ml NGF solution (experimental group) for 7 days was carried out according to the routine steps of scanning electron microscope sample processing. Observation under electron microscope ( figure 1 ); BCP immersed in 10μg / ml NGF solution for 7 days can be seen in the figure ( figure 1 b) A large number of proteins are attached to the surface, indicating that BCP has a strong adsorption capacity for NGF.

[0038] , BCP adsorption NGF quantitative detection

[0039] Put pure 10 μg / ml NGF solution (control group) and 10 μg / ml NGF solution soaked in BCP (experimental group) at 37°C in 5% CO 2 In the incubator, measure the protein concentration at 3 hours, 1 day, 2 days, 3 days, 4 days, 5 days, 6 days, and 7 days, and make a concentration change curve ( figure 2 );Depend on figure 2 It can be seen that BCP can adsorb most of the NGF in the solution after 7...

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Abstract

The invention discloses a production method of a growth factor supported porous biologic ceramic artificial bone scaffold, and belongs to the field of medical science and biomedical engineering. Qualitative and quantitative detection of a cobra venom nerve growth factor (NFG) adsorbed through a biphasic calcium phosphate ceramic (BCP) artificial bone scaffold and artificial bone scaffold function detection prove that the NGF supported BCP is in favor of realizing osteoblast proliferation and directed differentiation. The NGP supported BCP has a good ossification promotion effect, provides a possible method for producing osteoblast in vitro culture scaffolds, and makes researches of bioengineering materials be possible.

Description

technical field [0001] The invention belongs to the fields of medicine and biomedical engineering, and in particular relates to a method for preparing a porous bioceramic artificial bone support loaded with growth factors. Background technique [0002] Large bone defects are a difficult problem. Autologous bone transplantation, which used to be the gold standard, due to limited materials, postoperative subcutaneous hematoma, infection, pain, poor appearance, paresthesia and other complications (JakoiAM et al., 2015, MusculoskeletSurg99:171-178), researchers gradually turned their attention to The study of bioengineering materials comes up. Bioengineering materials, for bone biomimetic materials, can also be called artificial bone scaffold materials, which mainly play the role of local filling and directional induction of osteogenesis in bone defects. From the early research on natural materials such as allogeneic bone, collagen, coral, etc. to later synthetic materials suc...

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Application Information

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IPC IPC(8): A61L27/54A61L27/56A61L27/12
CPCA61L27/54A61L27/12A61L27/56A61L2300/414A61L2430/02
Inventor 赵劲民郑立靳攀黄力陆真慧
Owner GUANGXI MEDICAL UNIVERSITY
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