Collagen-based bilayer membrane material with directional release of loaded growth factors and its manufacturing method

Abstract

The invention relates to a collagen-based bilayer membrane material for directional release of growth factors and a manufacturing method of the collagen-based bilayer membrane material. The collagen-based bilayer membrane material is prepared from collagen, the growth factors, fibrinogen and thrombin; a dense layer collagen membrane and a loose layer collagen membrane are prepared, and the dense layer collagen membrane and the loose layer collagen membrane are bonded by virtue of fibrous protein, so that a bilayer membrane structure is constituted. The growth factors can be loaded on the surface of the membrane by a dosage of 1-10000ng of the growth factors per square centimeter; preferably, the growth factors are loaded on the bilayer membrane by a dosage of 30ng per square centimeter; and preferably, average thickness of the bilayer membrane is 1-5mm. The material has a bidirectional controlled-release effect on the loaded growth factors, so that directional release of the growth factors is achieved and the material can act on target tissues; and the material is applicable to restorative treatment of diseased and defective tissues. The growth factors, which are loaded on the bilayer collagen membrane prepared by the invention and have a targeted release effect, can be released to the target tissues in a directional mode and activity of the growth factors can be kept; and the bilayer membrane material can be used for guiding and inducing regeneration and repair of tissues.

Description

technical field

[0001] The invention relates to a collagen-based double-layer membrane material loaded with growth factors for directional release and a manufacturing method thereof. The material has a directional and controlled release effect on the growth factors loaded on it, and the growth factors released directionally act on target tissues. The material can be used for Repair of diseased and defective tissue. Background technique

[0002] Collagen is the most important water-insoluble fibrin outside the cell, and it is the skeleton of the extracellular matrix. Collagen provides tension and elasticity to cells in the extracellular matrix and plays a role in cell migration and development. It widely exists in the skin, bones, muscles, cartilage, joints, and hair tissues of the human body, and plays a triple anti-aging role of support, repair, and protection. Collagen is rich in content, almost accounting for more than 30% of the total protein weight. Glue is widely us...

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