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Composition for preserving epidermal growth factor activity for long time

An epidermal growth factor, long-term preservation technology, applied in the field of biotechnology and daily chemicals, can solve the problems of EGF thermal and microbial instability, easy degradation and inactivation, etc.

Active Publication Date: 2015-06-24
GUANGZHOU KENENG COSMETICS RES CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to overcome the problem that EGF is unstable to heat and microorganisms and is easily degraded and inactivated during production, transportation and storage, the object of the present invention is to provide a composition that preserves EGF activity for a long time

Method used

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  • Composition for preserving epidermal growth factor activity for long time
  • Composition for preserving epidermal growth factor activity for long time
  • Composition for preserving epidermal growth factor activity for long time

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Prepare the composition for long-term preservation of EGF of the present invention by the proportioning shown in table 1:

[0026] Table 1. Composition formula of the present invention (by mass percentage, total 100%)

[0027]

[0028]

[0029] Wherein, the EGF is EGF prepared by bioengineering fermentation, and its amino acid sequence is shown in SEQ ID No.1.

[0030] The preparation method of above-mentioned composition comprises the following steps:

[0031] A. Take 0.9g NaCl, 0.29g Na 2 HPO 4 12H 2 O, 0.0297g NaH 2 PO 4 2H 2 Dissolve O in 95ml of deionized water, adjust the pH to 7.4, and then set the volume to 100ml to make phosphate buffer, filter and sterilize with a 0.22um microporous membrane for later use;

[0032] B. Take 0.1 g of sodium hyaluronate (purchased from Dongying Foster Biological Engineering Co., Ltd.), add 5 g of glycerin and fully disperse;

[0033] C, get 0.5g phenoxyethanol (purchased from BASF), 1g hydrolyzed wheat protein (purc...

Embodiment 2

[0046] Prepare the composition for long-term preservation of EGF of the present invention by the proportioning of table 2:

[0047] Table 2. The formula of the composition of the present invention (by mass percentage, totaling 100%)

[0048]

[0049] Wherein, the EGF is EGF prepared by bioengineering fermentation, and its amino acid sequence is shown in SEQ ID No.1.

[0050] The preparation method of above-mentioned composition comprises the following steps:

[0051] A. Take 9g NaCl, 2.9g Na 2 HPO 4 12H 2 O, 0.297g NaH 2 PO 4 2H 2 Dissolve O in 950ml of deionized water, adjust the pH to 7.4, and then set the volume to 1000ml to make phosphate buffer, filter and sterilize with a 0.22um microporous membrane for later use;

[0052] B. Take 1g of sodium hyaluronate and add it into 50g of glycerin to fully disperse;

[0053] C, get 5g phenoxyethanol, 10g hydrolyzed wheat protein, 10g trehalose in the above-mentioned glycerin, add the above-mentioned phosphate buffer of 800...

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Abstract

The invention discloses a composition for preserving epidermal growth factor activity for a long time. The composition comprises a preservative, a protective agent and a phosphate buffer solution and preferably comprises the following components in percentage by mass: 0.00001-0.05% of EGF, 0.001-5.00% of the preservative and 0.01-25.00% of the protective agent and 0.01-0.1mol / L phosphate buffer solution with a pH value of 6-8 is used as the solvent. The preservative mainly comprises phenoxyethanol, DMDM hydantoin, benzyl alcohol, benzoic acid, methylisothiazolinone, propyl hydroxybenzoate and methylparaben; the protective agent mainly comprises hydrolyzed collagen, marine collagen, hydrolyzed wheat protein, sodium hyaluronate, amino acid, dextran and trehalose, ascorbic acid, hydrolyzed wheat protein, sodium hyaluronate, amino acid, glucan, mycose, ascorbic acid, water soluble vitamin E, chitosan and silk peptide; experiments prove that the composition can effectively alleviate the degradation of EGF and keep the bioactivity of EGF.

Description

technical field [0001] The invention relates to a composition for long-term preservation of epidermal growth factor activity, which belongs to the technical fields of biotechnology and daily chemicals. Background technique [0002] Epidermal growth factor (EGF) can promote the growth, division and metabolism of epidermal cells, epithelial cells and fibroblasts in the body, promote the growth of microvessels, and improve the microenvironment of cell growth. Therefore, it has a good repairing and caring effect on damaged skin, sensitive skin, traumatic skin and remodeled skin, especially for the repair of local damaged skin after skin resurfacing, which is popular in beauty salons, and can also prevent postoperative complications. occurrence of the disease. Just because of its good effect, less side effects, more safety, people pay more and more attention to it, adding EGF in cosmetics has become very common and popular. [0003] However, as a biologically active polypeptide...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/64A61K8/65A61K8/73A61Q19/00
Inventor 杨登亮项琪毛勇进黄亚东刘德海张卉邓燕柠李健安贝煜张敏静黄永彤张伟杰张楚标
Owner GUANGZHOU KENENG COSMETICS RES CO LTD
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