Injectable type self-healing hemostatic material as well as preparation method and application thereof

A hemostatic material and self-healing technology, applied in applications, surgical adhesives, medical science, etc., can solve problems such as inapplicability of fragile nerve or brain tissue, poor gel mechanical strength, and poor viscosity

Active Publication Date: 2017-07-28
SHENZHEN HUA NOVA BIOTECH LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, hemostatic products that achieve hemostasis through physical action, such as hemostatic gauze, sponge, etc., need to physically compress the bleeding wound during the hemostatic process, and at the same time, the material absorbs water in the blood to promote coagulation, and the hemostatic speed is slow and the effect is poor.
Moreover, this type of material is only effective for bleeding with a small flow rate, so it must be used with a hemostatic clamp, which makes the above-mentioned hemostatic material often require the surgeon to predict the amount of bleeding in advance or repeatedly use the hemostatic forceps
At the same time, traditional hemostatic materials (such as cotton gauze, bandages) are not ideal for the

Method used

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  • Injectable type self-healing hemostatic material as well as preparation method and application thereof
  • Injectable type self-healing hemostatic material as well as preparation method and application thereof
  • Injectable type self-healing hemostatic material as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047]Using type A gelatin as raw material, heat it in deionized water at 40°C to dissolve it, prepare a gelatin aqueous solution with a concentration of 5w / v%, adjust the pH value of the gelatin aqueous solution to 3, and then add ethanol three times the volume of the solution to the solution to generate Dispersion of gelatin microgel particles; add 25wt% glutaraldehyde aqueous solution to the dispersion respectively to crosslink the gelatin microgel particles, the amount of glutaraldehyde added is respectively 66 μL of 25wt% glutaraldehyde per gram of gelatin, The cross-linking reaction time is 12 hours, centrifuged and washed to obtain a dispersion of type A gelatin microgel particles with positive surface zeta potential. In the same way, a dispersion of B-type gelatin microgel particles with a negative surface zeta potential was prepared using B-type gelatin as a raw material. The particle size and zeta potential data of the prepared microgel measured by laser particle siz...

Embodiment 2

[0058] Use type A gelatin as the raw material, dissolve it under heating at 40°C, prepare a type A gelatin aqueous solution with a concentration of 5w / v%, adjust the pH value to 11, and then add 3.5 times the volume of ethanol to the solution to generate type A gelatin microparticles. Gel particle suspension; add 25wt% glutaraldehyde aqueous solution to the suspension to crosslink the gelatin microgel particles, the amount of glutaraldehyde added is 66 μL of 25wt% glutaraldehyde per g of gelatin, and the crosslinking reaction time is 12hr , followed by adding glycine to neutralize unreacted aldehyde groups, and centrifuging to obtain type A gelatin microgel particles. The particle size and surface zeta point parameters are shown in Table 4.

[0059] Prepare alginic acid microgel particles by emulsion method, the specific preparation method is as follows: add 1wt% sodium alginate aqueous solution dropwise into 50mM calcium chloride aqueous solution and continue high-speed stirri...

Embodiment 3

[0066] Use B-type gelatin as raw material, dissolve it under heating at 40°C, prepare a B-type gelatin aqueous solution with a concentration of 5w / v%, adjust the pH value to 3, and then add 3 times the volume of ethanol to the solution to generate B-type gelatin microparticles. Gel particle suspension; add 25wt% glutaraldehyde aqueous solution to the suspension to crosslink the gelatin microgel particles, the amount of glutaraldehyde added is 80 μL of 25wt% glutaraldehyde per g of gelatin, and the crosslinking reaction time is 12hr , repeatedly centrifuged and resuspended in deionized water to obtain type B gelatin microgel particles, the particle size and surface zeta point parameters are shown in Table 6.

[0067] The chitosan quaternary ammonium salt is dissolved in deionized water to prepare a 2.5w / v% chitosan quaternary ammonium salt aqueous solution. Disperse the gelatin B microgel particles in 20mM acetic acid aqueous solution, further fully blend the chitosan quaternar...

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Abstract

The invention discloses an injectable type self-healing hemostatic material as well as a preparation method and application thereof. The injectable type self-healing hemostatic material is obtained by self-assembling macromolecular microgel particles with surface charges through electrostatic interaction between the particles or the particles and macromolecules. The electrostatic action between the microgel particles or the particles and the macromolecules is physical crosslinking and has reversibility, so the hemostatic material prepared by the method has high injectability, self-healing capability and high mechanical strength, does not need to introduce chemical crosslinking reaction in the injection and solidification process, is different from chemical macromolecules, does not introduce a micromolecular crosslinking agent, has high biocompatibility, can be degraded and absorbed, does not have toxic or side effects, has high safety and makes wide application in the field of biomedicine possible.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and relates to a hemostatic material and a preparation method thereof. Specifically, the invention relates to a hemostatic material that can be directly applied to human and other breastfeeding patients caused by surgical operations (including minimally invasive operations) and trauma. The preparation method of gelatin-based hemostatic powder and hemostatic gel material for the purposes of hemostasis, wound sealing, tissue exudation reduction, tissue repair promotion and wound tissue protection for tissue and organ wounds of animals. Background technique [0002] During surgical operations, it is necessary to stop bleeding and seal wounds caused by different reasons. Therefore, hemostatic materials are a very important class of biomedical materials, which have important research and application significance. At present, the hemostatic materials commonly used in clinical practice can ...

Claims

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Application Information

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IPC IPC(8): A61L24/10A61L24/08A61L24/04A61L24/00
Inventor 王华楠
Owner SHENZHEN HUA NOVA BIOTECH LTD
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