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Preparation method of tissue-regeneration-promoting controlled-release multiple-growth-factor self-assembled coating

A growth factor, tissue regeneration technology, applied in the field of bio-regenerative medicine

Inactive Publication Date: 2014-04-30
TONGJI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In summary, in the reports of the existing literature, there is no self-assembled coating about the self-assembled coating for tissue regeneration and controlled release of multiple growth factors described in the present invention. Related Research Reports

Method used

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  • Preparation method of tissue-regeneration-promoting controlled-release multiple-growth-factor self-assembled coating
  • Preparation method of tissue-regeneration-promoting controlled-release multiple-growth-factor self-assembled coating
  • Preparation method of tissue-regeneration-promoting controlled-release multiple-growth-factor self-assembled coating

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Embodiment 1

[0048] The technical solutions of the present invention will be further described below in conjunction with the accompanying drawings and embodiments.

[0049] In step 1, PCL is used as a raw material and dissolved in chloroform to prepare a solution with a mass ratio of 8%. This solution was added to the electrospinning syringe controlled by the syringe pump, the voltage of the high voltage generator was set to 15KV, the collection distance was 16cm, the flow rate of the syringe was 0.5ml / min, and the electrospun film was collected on a square aluminum foil. After natural drying, it was washed several times with ethanol and deionized water, and then dried with nitrogen gas for use.

[0050] Step 2: Prepare PAH and PSS polyelectrolyte solutions with a concentration of 1 mg / ml, COL and ALG with a concentration of 0.3 mg / ml, and the NaCl content in the solution is 0.15M. The positively charged BMP-2 solution was dispersed in the above PAH solution, the concentration of BMP-2 wa...

Embodiment 2

[0068] In step 1, PLGA and collagen (collagen) were used as raw materials (mass ratio: 6:1), and dissolved in hexafluoropropanol to prepare a solution with a mass percentage of 7%. This solution was added to the electrospinning syringe controlled by the syringe pump, the voltage of the high voltage generator was set to 13KV, the collection distance was 14cm, the flow rate of the syringe was 0.02ml / min, and the electrospinning film was collected on the aluminum foil. After collection, it was dried in a vacuum oven (at a temperature of 35°C), and then the dried film was immersed in a 5% EDC solution, cross-linked at room temperature for 1 hour, and washed with ethanol and deionized water several times. Nitrogen dried for use.

[0069] Step 2, preparing a PAH and PAA polyelectrolyte solution with a concentration of 1 mg / ml, and the NaCl content in the solution is 0.15M. The DNCPs were dispersed in a 4mM HCl solution containing 0.1% human serum albumin at a concentration of 30ug / ...

Embodiment 3

[0073] Step 1, use gelatin (Gelatin) and modified chitosan (chitosan) as raw materials (mass ratio is 2:3), dissolve in N-N-dimethylacetamide (DMA), and add 1.0% (wt ) 2-Dimethylamino-2-benzyl-1-[4-(4-morpholinyl)phenyl]-1-butanone as photocuring initiator, at 365nm, 10 w / cm 2 Irradiate under ultraviolet light for 20 minutes to obtain a gel material. The obtained gel is washed under the action of deionized water to remove DMA therein to obtain a porous three-dimensional matrix scaffold. After collection, put it into a vacuum oven (at a temperature of 35°C) for drying, and then immerse the dried scaffold material in a solution of polyethyleneimine (PEI) with a concentration of 2 mg / ml, soak for 10 minutes, and wash with deionized water , dried with nitrogen gas for later use.

[0074] Step 2, prepare PAH and PAA polyelectrolyte solutions with a concentration of 2mg / ml, and the NaCl content in the solution is 0.2M. Disperse bFGF in a 4.5mM HCl solution containing 0.1% human s...

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Abstract

The invention belongs to the biological regenerative medicine field, and particularly relates to a preparation method of a tissue-regeneration-promoting controlled-release multiple-growth-factor self-assembled coating. The preparation method is as follows: using an organic polymer membrane and a three-dimensional scaffold as templates, and loading bone morphogenetic protein-2 (BMP2), dentin non-collagenous proteins (DNCPs), vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF) and other bioactive factors with tissue regeneration promotion effects by a supramolecular layer by layer self-assembly technique to form a certain layers of supramolecular biological film coating structure simultaneously embedding with different growth factors on the template surface. The tissue-regeneration-promoting functional coating prepared by the preparation method has good biological compatibility and stability, can improve the surface properties of materials, and can induce tissue regeneration by adjusting the release of the loaded growth factors.

Description

technical field [0001] The invention belongs to the field of biological regenerative medicine, and in particular relates to a preparation method of a self-assembly coating for promoting tissue regeneration and controlling the release of multiple growth factors. Background technique [0002] Tissue and organ dysfunction or defect seriously endangers human health and is one of the main causes of human disease and death. According to statistics, millions of surgeries are performed every year in the United States due to dysfunction or defect of various tissues and organs [1]. Every year in my country, millions of patients need skin transplantation due to burns, and as many as 1 million patients need artificial joint replacement due to various bone and cartilage injuries. Alternative treatment methods commonly used in clinic include allogeneic tissue transplantation, autologous tissue transplantation and synthetic tissue substitutes, but these methods have some shortcomings. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/44A61L27/54
Inventor 刘月华李文星陈静
Owner TONGJI UNIV
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