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Preparation method for immunosensor based on gamma-polyglutamic acid grafted dopamine and chitosan complex micelles

An immune sensor and polyglutamic acid technology, applied in the direction of instruments, scientific instruments, measuring devices, etc., can solve the problems of professional operators, cumbersome and complicated cleaning process, large background noise signal, etc., and achieve long analysis time and high specific surface area The effect of large and wide detection range

Active Publication Date: 2015-03-11
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many immunoassay methods for detecting tumor markers in serum, such as radioimmunoassay, chemiluminescence immunoassay, enzyme-linked immunoassay, fluorescence immunoassay and liposome immunoassay, etc. However, these methods It has the disadvantages of radiation hazard, large background noise signal, cumbersome and complicated cleaning process, long analysis time, expensive instruments and professional operators.

Method used

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  • Preparation method for immunosensor based on gamma-polyglutamic acid grafted dopamine and chitosan complex micelles
  • Preparation method for immunosensor based on gamma-polyglutamic acid grafted dopamine and chitosan complex micelles
  • Preparation method for immunosensor based on gamma-polyglutamic acid grafted dopamine and chitosan complex micelles

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Embodiment 1

[0043] A preparation method of an immunosensor based on gamma-polyglutamic acid grafted dopamine-chitosan composite micelles, comprising the following specific steps:

[0044] (1) Preparation of macromolecular micelles

[0045] Add 500 μL of 1.0 mg / mL chitosan acetic acid solution (with a mass fraction of 1% acetic acid aqueous solution as a solvent) dropwise into 20 mL of 0.2 mg / mL gamma-polyglutamic acid grafted dopamine solution, at a speed of 500 rpm Stir to make the chitosan and γ-polyglutamic acid grafted dopamine solution self-assemble through electrostatic interaction to form macromolecular micelles, continue stirring at a speed of 500rpm for 12h, so that the micellar polymers are assembled completely; The beam solution passes through a needle filter with a pore size of 800nm ​​to obtain a macromolecule micellar solution with a uniform particle size distribution, that is, a γ-polyglutamic acid grafted dopamine chitosan (γ-PGA-g-DACS) composite micelle solution;

[0046]...

Embodiment 2

[0056] A preparation method of an immunosensor based on gamma-polyglutamic acid grafted dopamine-chitosan composite micelles, comprising the following specific steps:

[0057] (1) Preparation of macromolecular micelles

[0058] Add 1mL of 0.5mg / mL chitosan hydrochloric acid solution (1% aqueous hydrochloric acid solution as a solvent) dropwise into 40mL of 0.1mg / mL gamma-polyglutamic acid grafted dopamine solution, stirring at a speed of 1000rpm , so that chitosan and γ-polyglutamic acid grafted dopamine solution self-assembled to form macromolecular micelles through electrostatic interaction, and continued to stir at a speed of 1000rpm for 5h, so that the micellar polymers were assembled completely; the obtained macromolecular micelles solution Through a needle filter with a pore size of 800nm, a macromolecule micellar solution with a uniform particle size distribution, that is, a γ-polyglutamic acid grafted dopamine chitosan (γ-PGA-g-DACS) composite micelle solution is obtai...

Embodiment 3

[0069] A preparation method of an immunosensor based on gamma-polyglutamic acid grafted dopamine-chitosan composite micelles, comprising the following specific steps:

[0070] (1) Preparation of macromolecular micelles

[0071] Add 1mL of 0.8mg / mL chitosan hydrochloric acid solution (1% aqueous hydrochloric acid solution as a solvent) dropwise into 50mL of 0.05mg / mL gamma-polyglutamic acid grafted dopamine solution, stirring at a speed of 700rpm , so that chitosan and γ-polyglutamic acid grafted dopamine solution self-assembled to form macromolecular micelles through electrostatic interaction, and continued to stir at a speed of 700rpm for 8h, so that the micellar polymers were assembled completely; the obtained macromolecular micelles The solution passes through a needle filter with a pore size of 800nm ​​to obtain a macromolecule micellar solution with a uniform particle size distribution, that is, a γ-polyglutamic acid grafted dopamine chitosan (γ-PGA-g-DACS) composite mice...

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Abstract

The invention discloses a preparation method for an immunosensor based on gamma-polyglutamic acid grafted dopamine and chitosan complex micelles. The preparation method comprises the following steps: preparing polymeric micelles; preparing polymeric micelle modified electrodes; preparing the immunosensor. According to the preparation method, the prepared polymeric micelles are very good in biocompatibility, the application of the polymeric micelles to the immunosensor can effectively increase the immobilization amount of antibodies and well keep the activity of biomacromolecules, and the prepared immunosensor has the advantages of high specificity, good stability, wide detection range and the like; the combination of a macromolecular self-assembly technology and an electrochemical sensor can be widely applied to immunoassay and extendedly applied to the fields of food safety, biological medicines, environmental protection monitoring and the like.

Description

technical field [0001] The invention relates to the field of electrochemical sensors, in particular to a method for preparing an electrode modified by macromolecule micelles-gamma-polyglutamic acid grafted with dopamine-chitosan composite micelles, and preparing it into an electrochemical immunosensor. Background technique [0002] February 4th, 2014 is "World Cancer Day". Nearly half of them occur in Asia, most of which are in China, where the number of new cancer cases ranks first; the report predicts that global cancer cases will show rapid growth, from 14 million in 2012 to 19 million in 2025 people, reaching 24 million by 2035. [0003] Clinically, the detection of tumor markers such as carcinoembryonic antigen (CEA), alpha-fetoprotein (AFP), carbohydrate antigen (CA125, CA19-9) can be used for colon cancer, lung cancer, pancreatic cancer, gastric cancer and other glandular malignancies. It has important reference value for judging the curative effect, disease develop...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574G01N27/327
CPCG01N27/327G01N33/574
Inventor 刘晓亚许升张荣莉罗静张翠歌赵鑫犇
Owner JIANGNAN UNIV
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