Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Self-assembled modified nano gold spherical array bio-sensitive film and preparation method thereof

A bio-sensitive film, nano-gold technology, applied in biological testing, measuring devices, material inspection products, etc., can solve the problem that the strong electrochemical activity of nano-gold particles is not conducive to the transmission of specific potential signals, and affects the uniformity and consistency of biological macromolecules. , sensor specificity weakening and other problems, to achieve the effect of eliminating non-specific sites, good spatial distribution and orientation, and maintaining immune activity

Inactive Publication Date: 2010-12-29
INST OF ELECTRONICS CHINESE ACAD OF SCI
View PDF0 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the active sites on the surface of gold nanoparticles are non-specific, and it is easy to introduce interference from non-specific signals, which weakens the specificity of the sensor.
Moreover, the poor dispersion of gold nanoparticles assembled on the substrate affects the uniformity and consistency of immobilization of biomacromolecules on the electrode surface.
In addition, according to the characteristics of selective ion transfer required by potentiometric sensors, the strong electrochemical activity of gold nanoparticles is not conducive to the transmission of specific potential signals.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Self-assembled modified nano gold spherical array bio-sensitive film and preparation method thereof
  • Self-assembled modified nano gold spherical array bio-sensitive film and preparation method thereof
  • Self-assembled modified nano gold spherical array bio-sensitive film and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] The following takes the determination of human immunoglobulin as an example to illustrate the preparation method of the biosensitive membrane immobilization method based on the hybrid self-assembled modified nano-gold spheroid array.

[0049] Miniature potentiometric immunosensors based on biosensitive membranes of hybrid self-assembled modified gold nanosphere arrays, such as figure 1 Shown:

[0050] The sensor is prepared by micro-nano-fabrication technology, including miniature working electrode a and reference electrode b, and "gold electrode-self-assembled monolayer-mixed self-assembled modified nano-gold sphere array-biological Macromolecules" Biomolecular Sensitive Membranes c.

[0051] The flow chart of the biosensitive membrane based on the mixed self-assembled modified nano-gold spheroid array is shown in Figure 2: first, the two mercapto compounds of mercaptopropionic acid and 11-mercaptoundecanoic acid were dissolved in deionized water or In the absolute e...

Embodiment 2

[0055] The following uses the determination of glycosylated hemoglobin as an example to illustrate the preparation method of the biosensitive membrane immobilization method based on the hybrid self-assembled modified nano-gold spheroid array. Glycosylated hemoglobin is the gold standard for diabetes detection.

[0056] Miniature potentiometric immunosensors based on biosensitive membranes of hybrid self-assembled modified gold nanosphere arrays, such as figure 1 Shown:

[0057] The sensor is prepared by micro-nano-fabrication technology, including miniature working electrode a and reference electrode b, and "gold electrode-self-assembled monolayer-mixed self-assembled modified nano-gold sphere array-biological Macromolecules" Biomolecular Sensitive Membranes c.

[0058] The flow chart of the biosensitive membrane based on the mixed self-assembled modified nano-gold spheroid array is shown in Figure 2: first, the two mercapto compounds of mercaptopropionic acid and 16-mercapt...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a self-assembled modified nano gold spherical array bio-sensitive film and a preparation method thereof. A nano gold particle spherical structure of which the surface is modified with a binary mixed self-assembled film is arranged on a self-assembled unimolecule layer modified gold electrode in an array form to fix biological macromolecules so as to form a bio-sensitive film. The bio-sensitive film is prepared by the following steps of: A) mixing two thiol solvents with different carbon chain lengths of which a functional group is carboxyl at one end according to an equal proportion so as to prepare thiol mixed solution; B) soaking a working electrode in the mixed solution of the step A; C) adding the same amount of or excessive thiol mixed solution into nano gold sol solution according to a molar ratio, and adding a surfactant to prepare mixed self-assembled film saturation modified nano gold sol; D) soaking the working electrode of the step B in the nano gold sol of the step C; and E) dripping solution of biological macromolecules on the surface of the working electrode of the step D, and standing to form the bio-sensitive film.

Description

technical field [0001] The invention belongs to the technical field of potentiometric immunosensors, and in particular relates to a biologically sensitive membrane based on mixed self-assembled modified nano-gold spheroid arrays and a preparation method. Background technique [0002] With the development of biotechnology, using the specific combination of antigen and antibody, immunosensors have received extensive research and attention due to their advantages of high specificity and high selectivity. At present, immunosensors include fluorescent immunosensors, optical fiber immunosensors, and enzyme-linked immunosensors. Fluorescent immunosensors, optical fiber immunosensors, and enzyme-linked immunosensors have realized biochemical trace detection, and made clinical immunological detection of trace biomass signals possible. [0003] Potentiometric immunosensors are developed based on the principle of ion-selective electrodes. The biological recognition response is conver...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N27/327G01N33/53
Inventor 夏善红薛茜男边超孙辑舟张虹
Owner INST OF ELECTRONICS CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com