Self-assembled modified nano gold spherical array bio-sensitive film and preparation method thereof

A bio-sensitive film, nano-gold technology, applied in biological testing, measuring devices, material inspection products, etc., can solve the problem that the strong electrochemical activity of nano-gold particles is not conducive to the transmission of specific potential signals, and affects the uniformity and consistency of biological macromolecules. , sensor specificity weakening and other problems, to achieve the effect of eliminating non-specific sites, good spatial distribution and orientation, and maintaining immune activity

Inactive Publication Date: 2010-12-29
INST OF ELECTRONICS CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the active sites on the surface of gold nanoparticles are non-specific, and it is easy to introduce interference from non-specific signals, which weakens the specificity of the sensor.
Moreover, the poor dispersion of gold nanoparticles assembled on the substrate affects the uniformity and consis

Method used

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  • Self-assembled modified nano gold spherical array bio-sensitive film and preparation method thereof
  • Self-assembled modified nano gold spherical array bio-sensitive film and preparation method thereof
  • Self-assembled modified nano gold spherical array bio-sensitive film and preparation method thereof

Examples

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Embodiment 1

[0048] The following takes the determination of human immunoglobulin as an example to illustrate the preparation method of the biosensitive membrane immobilization method based on the hybrid self-assembled modified nano-gold spheroid array.

[0049] Miniature potentiometric immunosensors based on biosensitive membranes of hybrid self-assembled modified gold nanosphere arrays, such as figure 1 Shown:

[0050] The sensor is prepared by micro-nano-fabrication technology, including miniature working electrode a and reference electrode b, and "gold electrode-self-assembled monolayer-mixed self-assembled modified nano-gold sphere array-biological Macromolecules" Biomolecular Sensitive Membranes c.

[0051] The flow chart of the biosensitive membrane based on the mixed self-assembled modified nano-gold spheroid array is shown in Figure 2: first, the two mercapto compounds of mercaptopropionic acid and 11-mercaptoundecanoic acid were dissolved in deionized water or In the absolute e...

Embodiment 2

[0055] The following uses the determination of glycosylated hemoglobin as an example to illustrate the preparation method of the biosensitive membrane immobilization method based on the hybrid self-assembled modified nano-gold spheroid array. Glycosylated hemoglobin is the gold standard for diabetes detection.

[0056] Miniature potentiometric immunosensors based on biosensitive membranes of hybrid self-assembled modified gold nanosphere arrays, such as figure 1 Shown:

[0057] The sensor is prepared by micro-nano-fabrication technology, including miniature working electrode a and reference electrode b, and "gold electrode-self-assembled monolayer-mixed self-assembled modified nano-gold sphere array-biological Macromolecules" Biomolecular Sensitive Membranes c.

[0058] The flow chart of the biosensitive membrane based on the mixed self-assembled modified nano-gold spheroid array is shown in Figure 2: first, the two mercapto compounds of mercaptopropionic acid and 16-mercapt...

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Abstract

The invention discloses a self-assembled modified nano gold spherical array bio-sensitive film and a preparation method thereof. A nano gold particle spherical structure of which the surface is modified with a binary mixed self-assembled film is arranged on a self-assembled unimolecule layer modified gold electrode in an array form to fix biological macromolecules so as to form a bio-sensitive film. The bio-sensitive film is prepared by the following steps of: A) mixing two thiol solvents with different carbon chain lengths of which a functional group is carboxyl at one end according to an equal proportion so as to prepare thiol mixed solution; B) soaking a working electrode in the mixed solution of the step A; C) adding the same amount of or excessive thiol mixed solution into nano gold sol solution according to a molar ratio, and adding a surfactant to prepare mixed self-assembled film saturation modified nano gold sol; D) soaking the working electrode of the step B in the nano gold sol of the step C; and E) dripping solution of biological macromolecules on the surface of the working electrode of the step D, and standing to form the bio-sensitive film.

Description

technical field [0001] The invention belongs to the technical field of potentiometric immunosensors, and in particular relates to a biologically sensitive membrane based on mixed self-assembled modified nano-gold spheroid arrays and a preparation method. Background technique [0002] With the development of biotechnology, using the specific combination of antigen and antibody, immunosensors have received extensive research and attention due to their advantages of high specificity and high selectivity. At present, immunosensors include fluorescent immunosensors, optical fiber immunosensors, and enzyme-linked immunosensors. Fluorescent immunosensors, optical fiber immunosensors, and enzyme-linked immunosensors have realized biochemical trace detection, and made clinical immunological detection of trace biomass signals possible. [0003] Potentiometric immunosensors are developed based on the principle of ion-selective electrodes. The biological recognition response is conver...

Claims

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Application Information

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IPC IPC(8): G01N27/327G01N33/53
Inventor 夏善红薛茜男边超孙辑舟张虹
Owner INST OF ELECTRONICS CHINESE ACAD OF SCI
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