Inorganic carrier used for cell immobilization and immobilized nitrobacteria prepared by same

Abstract

The invention provides a cheap inorganic porous adsorption carrier capable of being used for cell immobilization. Clay is fired to prepare the carrier; the carrier has the characteristics of porosity, high mechanical strength, stable properties, low price, large cell immobilization quantity and the like, is simple in cell immobilization operation, is non-toxic for microbial cells and can be reused; and the carrier can be used for industrial-scale cell immobilization. The carrier is used to immobilize nitrobacteria so as to prepare immobilized nitrobacteria used for converting ammonium in a water body to nitrate; and the conversion speed of the obtained immobilized nitrobacteria to ammonium is high, and the efficiency is high. The method for converting ammonium with the immobilized nitrobacteria comprises the following steps: the immobilized nitrobacteria are placed in a netted container, the netted container is placed in an ammonium sulfate solution or ammonium-containing water body, and after a certain time period, the immobilized nitrobacteria can convert ammonium to nitric nitrogen. The immobilized nitrobacteria in the netted container can be reused. After the immobilized nitrobacteria are respectively placed in a 5g/L ammonium sulfate solution and a 20g/L ammonium sulfate solution for 4h, the average conversion rates of ammonium nitrogen are respectively 79.7%-83.6% and 86.5%-86.7%. After being stored for half a year at 4 DEG C, the nitrification ability of the immobilized nitrobacteria is not reduced.

Description

technical field [0001] The invention belongs to the technical field of immobilized cells, and mainly relates to a cheap inorganic carrier that can be used for cell immobilization. Background technique [0002] Immobilized microorganism technology is a new technology that fixes free microbial cells by chemical and physical methods to make them immobilized cells that are insoluble in water but still have high biological activity. That is, by artificially adsorbing specific microorganisms on the surface of the carrier or sealing them in a porous inert carrier, the loss of microorganisms can be reduced, the density of microorganisms in the carrier can be increased, and the speed of biochemical reactions can be accelerated. Immobilized cell technology can realize the reuse of cells, reduce costs and improve efficiency. The choice of immobilization carrier is the key to the technology of microorganism immobilization. The carrier suitable for cell immobilization should have the f...

AI Technical Summary

Problems solved by technology

Due to the high price of organic polymer carriers and composite carriers, they cannot be used for immobilization of cells in large-scale production

Small granular carriers such as porous glass, diatomaceous earth, activated carbon, and quartz sand among inorganic carriers are difficult to recover and are also difficult to use for cell immobilization required for large-scale cell transformation

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