Paper element micro-fluidic chip proteolysis reactor and preparation method thereof

A microfluidic chip and proteolysis technology are applied in the field of a paper core microfluidic chip proteolysis reactor and its preparation, which can solve the problems of easy loss and affect the effect of enzymolysis, and achieve low cost, cost reduction and simple equipment. Effect

Inactive Publication Date: 2015-09-16
FUDAN UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

The problem is that the enzyme is not fixed by covalent bonds, so it is easy to lose and affect the effect of enzymati...

Method used

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  • Paper element micro-fluidic chip proteolysis reactor and preparation method thereof
  • Paper element micro-fluidic chip proteolysis reactor and preparation method thereof
  • Paper element micro-fluidic chip proteolysis reactor and preparation method thereof

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Embodiment Construction

[0042] Further describe the present invention below by embodiment and accompanying drawing:

[0043] 1. Single-channel paper core microfluidic chip proteolysis reactor and its preparation method

[0044] Cut the plexiglass plate with a thickness of 1 mm into small pieces 2 or 4 of the chip size (60 mm×16 mm×1 mm) to be prepared. Cut qualitative filter paper with a thickness of 200 μm into paper strips 1 with a length of 54 mm and a width of 2 mm by a blade. Such as figure 1 As shown, after wetting with water, paste it on the plexiglass sheet 2 cut into the size of the chip. After drying, cover another plexiglass sheet 4 of the same size with a solution connection hole with a diameter of 2 mm, and clamp it on the glass sheet. 3 and 6 (76.2 mm × 25 mm × 1.2 mm), and then use a spring-driven pressure device to apply an external pressure 5, the pressure is 6 kg / cm2. Then, the device was placed in a blast oven at a temperature of 130°C and heated for 10 minutes. The paper core ...

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Abstract

The invention belongs to the technical field of a micro-fluidic chip, and particularly relates to a paper element micro-fluidic chip proteolysis reactor and a preparation method thereof. The preparation method comprises the following steps: cutting a piece of filter paper into strips or a needed shape, wetting the filter paper, then adhering the filter paper onto a thermoplastic transparent plastic sheet which is cut into the size of a chip, airing the filter paper, covering the filter paper with a piece of plastic sheet in the same size with solution connection holes, and clamping the filter paper between two glass sheets, and then pressing by utilizing a clamp; and heating for 5 to 15 minutes in a drying oven with temperature of 110 to 180 DEG C, encapsulating a paper element between two plastic sheets, and obtaining a paper element micro-fluidic chip with a paper fiber filling channel, wherein the width of the channel with the paper element is 0.5mm to 3mm; and then filling the channel with a periodic acid solution, oxidizing cellulose in the paper element into aldehyde groups, cleaning, and injecting such a protease solution as trypsin solution so as to fix the protease by covalent bonds to obtain a proteolysis micro-fluidic chip. The reactor is simple and convenient to process, short in enzymolysis time and small in sample dosage.

Description

technical field [0001] The invention belongs to the technical field of microfluidic chips, and in particular relates to a paper-core microfluidic chip proteolysis reactor and a preparation method thereof. Background technique [0002] Since Manz and Widmer[1] of Ciba-Geigy in Switzerland first proposed the micro-total analysis system (μ-TAS) in 1990, the microfluidic chip is characterized by its small size, controllable liquid flow, high separation efficiency, reagents and samples. The advantages of small amount, fast analysis speed, easy automation of operation, low consumption and high integration have attracted widespread attention from experts in the fields of analytical chemistry and biomedicine at home and abroad. Based on the rapid development of its methodological research, microfluidic chips It has shown good application prospects in the fields of biomedical research, clinical diagnosis, drug analysis, food safety, environmental monitoring, forensic medicine and mil...

Claims

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Application Information

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IPC IPC(8): C12M1/40B01L3/00
CPCC12M21/18B01L3/502707C12M23/16
Inventor 陈刚张鲁雁陈琪雯
Owner FUDAN UNIV
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