Preparation and application of electrochemical luminescence sensor for detecting procalcitonin based on energy transfer between g-C3N4 and CuO
A technology of procalcitonin and energy transfer, applied in the direction of material electrochemical variables, scientific instruments, instruments, etc., can solve the problems of narrow detection range and time-consuming, and achieve high sensitivity, wide linear range and good reproducibility Effect
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Embodiment 1
[0032] Embodiment 1 A kind based on g-C 3 N 4 Preparation and Application of Electrochemiluminescence Sensor for Detection of Procalcitonin by Energy Transfer Between CuO and CuO
[0033] (1) with Al 2 o 3 The glassy carbon electrode with a diameter of 4 mm was polished with polishing powder, cleaned with ultrapure water, and 6 µL, 0.25 mg mL -1 Au and carbon nanotube-modified g-C 3 N 4 Composite g-C 3 N 4 -CNT@Au solution was added dropwise to the surface of the electrode, and dried at room temperature to form a film;
[0034] (2) Add 6 µL and 1 µg mL dropwise in sequence -1 Procalcitonin capture antibody, 3 µL, 0.1% BSA solution on the surface of the electrode, washed with ultrapure water, and dried at room temperature;
[0035] (3) Add 6 µL, 0.0001~10 ng mL dropwise -1 A series of procalcitonin antigens of different concentrations were applied to the electrode surface, incubated for 2 h, rinsed with ultrapure water, and dried at room temperature;
[0036] (4) Dr...
Embodiment 2
[0037] Embodiment 2 A kind based on g-C 3 N 4 Preparation and Application of Electrochemiluminescence Sensor for Detection of Procalcitonin by Energy Transfer Between CuO and CuO
[0038] (1) with Al 2 o 3 Grind the glassy carbon electrode with a diameter of 4 mm with polishing powder, clean it with ultrapure water, and distill 6 µL, 1.0 mg mL -1 Au and carbon nanotube-modified g-C 3 N 4 Composite g-C 3 N 4 -CNT@Au solution was added dropwise to the surface of the electrode, and dried at room temperature to form a film;
[0039] (2) Add 6 µL and 3 µg mL dropwise in sequence -1 Procalcitonin capture antibody, 3 µL, 0.1% BSA solution on the surface of the electrode, washed with ultrapure water, and dried at room temperature;
[0040] (3) Add 6 µL, 0.0001~10 ng mL dropwise -1 A series of procalcitonin antigens of different concentrations were applied to the electrode surface, incubated for 2 h, rinsed with ultrapure water, and dried at room temperature;
[0041] (4) Dr...
Embodiment 3
[0042] Embodiment 3 A kind based on g-C 3 N 4 Preparation and Application of Electrochemiluminescence Sensor for Detection of Procalcitonin by Energy Transfer Between CuO and CuO
[0043] (1) with Al 2 o 3 The glassy carbon electrode with a diameter of 4 mm was polished with polishing powder, cleaned with ultrapure water, and 6 µL, 2.0 mg mL -1 Au and carbon nanotube-modified g-C 3 N 4 Composite g-C 3 N 4 -CNT@Au solution was added dropwise to the surface of the electrode, and dried at room temperature to form a film;
[0044] (2) Add 6 µL, 5 µg mL dropwise in sequence -1 Procalcitonin capture antibody, 3 µL, 0.1% BSA solution on the surface of the electrode, washed with ultrapure water, and dried at room temperature;
[0045] (3) Add 6 µL, 0.0001~10 ng mL dropwise -1 A series of procalcitonin antigens of different concentrations were applied to the electrode surface, incubated for 2 h, rinsed with ultrapure water, and dried at room temperature;
[0046] (4) Drop 6 ...
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