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Preparation and application of electrochemical luminescence sensor for detecting procalcitonin based on energy transfer between g-C3N4 and CuO

A technology of procalcitonin and energy transfer, applied in the direction of material electrochemical variables, scientific instruments, instruments, etc., can solve the problems of narrow detection range and time-consuming, and achieve high sensitivity, wide linear range and good reproducibility Effect

Active Publication Date: 2020-01-14
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above methods have some disadvantages, such as the need to consume a lot of time or the detection range is narrow, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1 A kind based on g-C 3 N 4 Preparation and Application of Electrochemiluminescence Sensor for Detection of Procalcitonin by Energy Transfer Between CuO and CuO

[0033] (1) with Al 2 o 3 The glassy carbon electrode with a diameter of 4 mm was polished with polishing powder, cleaned with ultrapure water, and 6 µL, 0.25 mg mL -1 Au and carbon nanotube-modified g-C 3 N 4 Composite g-C 3 N 4 -CNT@Au solution was added dropwise to the surface of the electrode, and dried at room temperature to form a film;

[0034] (2) Add 6 µL and 1 µg mL dropwise in sequence -1 Procalcitonin capture antibody, 3 µL, 0.1% BSA solution on the surface of the electrode, washed with ultrapure water, and dried at room temperature;

[0035] (3) Add 6 µL, 0.0001~10 ng mL dropwise -1 A series of procalcitonin antigens of different concentrations were applied to the electrode surface, incubated for 2 h, rinsed with ultrapure water, and dried at room temperature;

[0036] (4) Dr...

Embodiment 2

[0037] Embodiment 2 A kind based on g-C 3 N 4 Preparation and Application of Electrochemiluminescence Sensor for Detection of Procalcitonin by Energy Transfer Between CuO and CuO

[0038] (1) with Al 2 o 3 Grind the glassy carbon electrode with a diameter of 4 mm with polishing powder, clean it with ultrapure water, and distill 6 µL, 1.0 mg mL -1 Au and carbon nanotube-modified g-C 3 N 4 Composite g-C 3 N 4 -CNT@Au solution was added dropwise to the surface of the electrode, and dried at room temperature to form a film;

[0039] (2) Add 6 µL and 3 µg mL dropwise in sequence -1 Procalcitonin capture antibody, 3 µL, 0.1% BSA solution on the surface of the electrode, washed with ultrapure water, and dried at room temperature;

[0040] (3) Add 6 µL, 0.0001~10 ng mL dropwise -1 A series of procalcitonin antigens of different concentrations were applied to the electrode surface, incubated for 2 h, rinsed with ultrapure water, and dried at room temperature;

[0041] (4) Dr...

Embodiment 3

[0042] Embodiment 3 A kind based on g-C 3 N 4 Preparation and Application of Electrochemiluminescence Sensor for Detection of Procalcitonin by Energy Transfer Between CuO and CuO

[0043] (1) with Al 2 o 3 The glassy carbon electrode with a diameter of 4 mm was polished with polishing powder, cleaned with ultrapure water, and 6 µL, 2.0 mg mL -1 Au and carbon nanotube-modified g-C 3 N 4 Composite g-C 3 N 4 -CNT@Au solution was added dropwise to the surface of the electrode, and dried at room temperature to form a film;

[0044] (2) Add 6 µL, 5 µg mL dropwise in sequence -1 Procalcitonin capture antibody, 3 µL, 0.1% BSA solution on the surface of the electrode, washed with ultrapure water, and dried at room temperature;

[0045] (3) Add 6 µL, 0.0001~10 ng mL dropwise -1 A series of procalcitonin antigens of different concentrations were applied to the electrode surface, incubated for 2 h, rinsed with ultrapure water, and dried at room temperature;

[0046] (4) Drop 6 ...

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Abstract

The invention discloses preparation and an application of an electrochemical luminescence sensor for detecting procalcitonin based on energy transfer between g-C3N4 and CuO, and belongs to the technical field of construction of novel sensors. Based on the good specificity between antigen and antibody, the sensor utilizes Au and carbon nanotube modified g-C3N4 composite material g-C3N4-CNT@Au as asubstrate luminescent material and polydopamine coated CuO as a quenching agent, and the electrochemical luminescence sensor is constructed through layer-by-layer self-assembly. The electrochemical luminescence sensor constructed by the invention has a wider detection range, higher sensitivity and lower detection limit, and has important significance for detection of procalcitonin.

Description

technical field [0001] A g-C based 3 N 4 Preparation and application of energy transfer detection procalcitonin electrochemiluminescence sensor between CuO and CuO; the present invention is g-C modified with Au and carbon nanotubes 3 N 4 Composite g-C 3 N 4 -CNT@Au as the substrate luminescent material, polydopamine-wrapped CuO as the quencher, and the electrochemical luminescence sensor constructed by layer-by-layer self-assembly can realize the specific detection of procalcitonin, which belongs to the field of new sensor construction technology. Background technique [0002] Worldwide, more than 210,000 patients die from sepsis every year. The concentration of procalcitonin in healthy individuals is very low, but the concentration of procalcitonin in the serum of patients with sepsis will gradually increase with the development of the disease. Procalcitonin is therefore considered to be one of the important markers for the diagnosis of sepsis, and sensitive detection...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26G01N27/30G01N27/327
CPCG01N27/26G01N27/30G01N27/3278
Inventor 胡丽华宋翠李小建师腾飞张勇吴丹马洪敏范大伟魏琴
Owner UNIV OF JINAN
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