Electrochemiluminescence sensing method used for histamine detection

Abstract

The ion exchange effect between a cation-containing polymeric material Nafion film modified glassy carbon electrode and cations in ruthenium terpyridine is used to immobilize ruthenium terpyridine to the surface of the electrode and dope the Nafion film with multi-walled carbon nano-tubes and zinc oxide nano-particles. The promotion effect of amido groups on the electrochemiluminescence of ruthenium terpyridine realizes histamine detection. The method using the MWCNTs/ZnO/Nafion compound film modified electrode to immobilize the ruthenium terpyridine has the advantages of reduction of the use amount of the ruthenium terpyridine, reduction of the cost of experiments, reduction of harms to environment and improvement of the stability and the repeated utilization rate of the experiments. The electrochemiluminescence sensing method used for histamine detection also has the advantages of wide application range, low detection limit and good stability and repeatability, and can be applied to detection of histamine in a practical sample.

Description

technical field [0001] The invention relates to an electrochemical luminescence sensing method for detecting histamine, belonging to the field of electrochemical sensors. Background technique [0002] Histamine, also known as: histamine dihydrochloride, histamine, etc., the molecular formula is: C 5 h 9 N 3 . Histamine is a self-active substance and one of many biogenic amines, which can be formed by decarboxylation of histidine in living organisms. A small amount of histamine has certain benefits to the body, it can properly regulate nerve activity and control blood pressure. However, in some foods, such as fish, fermented foods, wine, beverages, etc., histamine will be formed under the action of histidine decarboxylase. Excessive histamine in the body will cause harm and cause body poisoning. When the amount of histamine ingested by the body is greater than 0.1g, allergic food poisoning will be caused. Therefore, it is very important to strengthen the detection of h...

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Problems solved by technology

[0005] The present invention utilizes the polymer material Nafion film containing cations to modify the glassy carbon electrode, fixes ruthenium terpyridine to the surface of the electrode through ion exchange with the cations on ruthenium terpyridine, and at the same time, incorporates two kinds of The nanomaterials are multi-walled carbon nanotubes and nano-zinc oxide particles, which effectively solve the shortcomings of the Nafion membrane structure being dense and not conducive to the passage of substances

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