Preparation method of electrochemical sensor for detecting 2,4,6-trichlorophenol and analysis method

A trichlorophenol and electrochemical technology is applied in the field of preparation of electrochemical sensors, which can solve the problems of difficulty in meeting detection requirements, poor stability of natural enzymes, and being easily affected by the environment, and achieves improved fixed amount, low cost, and high sensitivity. Effect

Inactive Publication Date: 2018-06-22
DAFENG YUELONG CHEM
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Problems solved by technology

In traditional analysis, natural enzymes have poor stability and are easily affected by the environment
The existing electrochemical method for detecting 2,4,6-trichlorophenol has shortcomings such as insufficient detection limit and difficulty in meeting the requirements of actual sample detection.

Method used

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  • Preparation method of electrochemical sensor for detecting 2,4,6-trichlorophenol and analysis method
  • Preparation method of electrochemical sensor for detecting 2,4,6-trichlorophenol and analysis method
  • Preparation method of electrochemical sensor for detecting 2,4,6-trichlorophenol and analysis method

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Embodiment 1

[0031] Preparation of Cu-MOF-74 crystals: Weigh 0.13g of 2,5-dihydroxyterephthalic acid and 0.33g of copper nitrate trihydrate and dissolve them in 20mL of N,N-dimethylformamide, and ultrasonically dissolve them fully for 5min A clear bright green solution was obtained, then 1 mL of isopropanol was added, and mixed evenly to obtain a mixed solution. The mixed solution was placed in a 50 mL reaction kettle, and the temperature was raised to 80° C. for 18 hours in a vacuum environment. The reaction formula is as follows:

[0032]

[0033] After the reaction, it was naturally cooled to room temperature to obtain reddish needle-like Cu-MOF-74 crystals, which were washed several times with DMF to remove unreacted copper nitrate and 2,5-dihydroxyterephthalic acid. Soak the Cu-MOF-74 crystals in 100 mL of absolute ethanol for 4 days, replace the ethanol solution every 24 hours, then dry the Cu-MOF-74 crystals in a vacuum environment at 150 °C for 5 hours and store them in an iner...

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Abstract

The invention provides a preparation method of an electrochemical sensor for detecting 2,4,6-trichlorophenol. The preparation method comprises steps as follows: Cu-MOF-74 crystals are prepared from 2,5-dihydroxyterephthalic acid and copper nitrate trihydrate with a hydrothermal synthesis method; hemin is dissolved in a methanol aqueous solution, and a hemin stock solution is prepared; the Cu-MOF-74 crystals and the hemin stock solution are mixed, hemin and Cu-MOF-74 are subjected to coordination, hemin is immobilized on surface of Cu-MOF-74 and in pore channels, and a hemin/Cu-MOF-74 compositematerial is prepared; the hemin/Cu-MOF-74 composite material is dispersed in DMF, an electrode modification solution is prepared and dropwise applied to the surface of a polished glassy carbon electrode, then the glassy carbon electrode is put in a dryer and dried at room temperature, and the electrochemical sensor for detecting 2,4,6-trichlorophenol is obtained. The prepared hemin/Cu-MOF-74 electrochemical sensor has lower detection limit, can flexibly detect and measure 2,4,6-trichlorophenol in an actual sample, and is simple in technical operation, high in sensitivity and low in cost.

Description

technical field [0001] The invention belongs to a preparation method and an analysis method of an electrochemical sensor, and more specifically relates to a preparation and analysis method of an electrochemical sensor using hemin immobilized enzyme to catalyze and detect 2,4,6-trichlorophenol. Background technique [0002] The existing detection method of 2,4,6-trichlorophenol (2,4,6-TCP) has the disadvantages of cumbersome sample pretreatment process, long detection time and high instrument cost. Hemin easily forms dimers and polymers in aqueous solution and loses its catalytic activity. In traditional analysis, natural enzymes have poor stability and are easily affected by the environment. The existing electrochemical methods for the detection of 2,4,6-trichlorophenol have shortcomings such as insufficient detection limits and difficulty in meeting the requirements of actual sample detection. Contents of the invention [0003] The object of the present invention is to ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/48
CPCG01N27/48
Inventor 王璐
Owner DAFENG YUELONG CHEM
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