Method of removing antibiotic from water body with hollow mesoporous carbon immobilized laccase

A technology for immobilizing laccase and mesoporous carbon, applied in the direction of immobilizing on or in inorganic carriers, chemical instruments and methods, natural water treatment, etc., can solve the problems of low immobilization efficiency and instability, and achieve stable protection performance, improve efficiency, and improve the effect of transmission capacity

Active Publication Date: 2018-06-22
HUNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Conventional immobilization carriers include activated carbon, chitosan, porous silica gel and other materials, but these materials have disadvantages such as low immobilization efficiency and instability

Method used

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  • Method of removing antibiotic from water body with hollow mesoporous carbon immobilized laccase
  • Method of removing antibiotic from water body with hollow mesoporous carbon immobilized laccase
  • Method of removing antibiotic from water body with hollow mesoporous carbon immobilized laccase

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Experimental program
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Effect test

Embodiment 1

[0044] A kind of hollow mesoporous carbon immobilized laccase (HMCs-Lac and HMCs-NH) of the present invention 2 -GTA-Lac) removes the method for tetracycline hydrochloride (TCH) in water body, comprises the following steps:

[0045] Take 20 mg of hollow mesoporous carbon-immobilized laccase and add it to 100 mL of citric acid-sodium citrate buffer solution (0.1 M, pH=4.5) containing 50 mg / L tetracycline hydrochloride (TCH), and place each mixture at 30 ° C. In a shaking incubator at 150 rpm for 3 hours, the removal of tetracycline hydrochloride (TCH) was completed.

[0046] Among them, the structure of the hollow mesoporous carbon-immobilized laccase can be that the laccase is directly physically adsorbed in the hollow cavity of the hollow mesoporous carbon (referred to as HMCs-Lac), and the laccase utilizes glutaraldehyde, through covalent cross-linking Hollow mesoporous carbon-immobilized laccase immobilized on hollow mesoporous carbon (HMCs-NH 2 -GTA-Lac) both.

[0047] ...

Embodiment 2

[0085] Two kinds of hollow mesoporous carbon-immobilized laccases (inactivated HMCs-Lac and inactivated HMCs-NH 2 -GTA-Lac) and two kinds of hollow mesoporous carbon-immobilized laccases (HMCs-Lac and HMCs-NH 2 -GTA-Lac) removes the application research of tetracycline hydrochloride (TCH) pollutant in the water body, concrete steps are as follows:

[0086] Two hollow mesoporous carbon-immobilized laccases (inactivated Cs-Lac and inactivated HMCs-NH 2 -GTA-Lac) and two non-inactivated hollow mesoporous carbon-immobilized laccases (HMCs-Lac and HMCs-NH 2 -GTA-Lac) each 20mg was added to 100mL citric acid-sodium citrate buffer solution (0.1M, pH=4.5) containing 50mg / L tetracycline hydrochloride (TCH) The shaking incubator was removed for 3 hours, during which samples were taken at different times to determine the remaining concentration of TCH.

[0087] The concentration of TCH was measured at 357 nm using a UV-Vis spectrophotometer (UV-2550; Shimadzu company, Japan).

[0088...

Embodiment 3

[0093] A kind of method utilizing hollow mesoporous carbon immobilized laccase of the present invention to remove ciprofloxacin hydrochloride (CPH) in water body may further comprise the steps:

[0094] Take 20 mg of hollow mesoporous carbon-immobilized laccase and add it to 100 mL of citric acid-sodium citrate buffer solution (0.1M, pH=4.5) containing 50 mg / L ciprofloxacin hydrochloride (CPH), and place each mixture in 30° C., 150 rpm in a shaking incubator for 3 hours to complete the removal of ciprofloxacin hydrochloride (CPH).

[0095] Among them, the structure of hollow mesoporous carbon-immobilized laccase can be that laccase is directly physically adsorbed in the hollow cavity of hollow mesoporous carbon (referred to as HMCs-Lac), and laccase is immobilized by covalent cross-linking using glutaraldehyde. Hollow mesoporous carbon immobilized laccase (HMCs-NH 2 -GTA-Lac) both. The preparation method of the above two hollow mesoporous carbon-immobilized laccases with dif...

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Abstract

The invention discloses a method of removing an antibiotic from a water body with hollow mesoporous carbon immobilized laccase. The method comprises the following steps of: adding hollow mesoporous carbon immobilized laccase into a citric acid-sodium citrate buffer solution containing the antibiotic for oscillating reaction, allowing the antibiotic to enter a hollow inner cavity through mesoporouschannels on a hollow mesoporous carbon shell and be absorbed and degraded by laccase immobilized by the hollow inner cavity, and accomplishing the removal of the antibiotic. The method of removing the antibiotic from the water body with hollow mesoporous carbon immobilized laccase has the advantages that the method is simple, effective, easy to implement and high in safety and causes no secondarypollution.

Description

technical field [0001] The invention belongs to the technical field of sewage treatment, and in particular relates to a method for removing antibiotics in water by immobilizing laccase with hollow mesoporous carbon. Background technique [0002] Antibiotics are a type of secondary metabolites produced by microorganisms (including bacteria, fungi, actinomycetes) or higher animals and plants in the course of life, which have anti-pathogen or other activities, and can interfere with the development and function of other living cells. chemicals. In recent decades, since antibiotics are widely used in medicine, animal husbandry and aquaculture, etc., excessive and incomplete metabolism make antibiotics often detected in natural water, sewage, soil and other environmental media. These non-metabolized antibiotics are likely to affect the development of biological cells, the circulation of ecosystems and promote the reproduction of drug-resistant pathogens, thereby adversely affect...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34C12N11/14C02F103/34
Inventor 邵彬彬刘智峰曾光明李志刚刘洋刘玉杰蒋艺林
Owner HUNAN UNIV
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