Preparation method of immunosensor based on chitosan-gold hybrid particles
An immunosensor, chitosan technology, applied in the field of preparing electrochemical immunosensors to achieve the effects of improving electrical conductivity, excellent stability and biocompatibility, and improving long-term adhesion
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Embodiment 1
[0041] A preparation method of an immunosensor based on chitosan-gold hybrid particles, comprising the following specific steps:
[0042] (1) Synthesis of catechol-modified chitosan
[0043] Disperse 1.0g chitosan (CS) in 100mL pH 4.0 aqueous solution, stir for 12h until completely dissolved to form chitosan aqueous solution; take 2.183g caffeic acid (CAA) and 1.162g 1-ethyl-(3-di Methylaminopropyl) carbodiimide hydrochloride (EDC·HCl) was dissolved in a mixed solvent of ethanol and water to form a caffeic acid solution, and then the catechol derivative solution was added dropwise to the chitosan sugar aqueous solution; adjust the pH of the mixed reaction solution to 5.5, and react in an ice-water bath for 12 hours; finally, the reaction solution was dialyzed against acidic water for 12 hours, freeze-dried to obtain caffeic acid-modified chitosan CS-CAA, and stored in the dark for future use;
[0044] (2) Preparation of modified chitosan-gold hybrid nanoparticles
[0045] Sc...
Embodiment 2
[0054] A preparation method of an immunosensor based on chitosan-gold hybrid particles, comprising the following specific steps:
[0055] (1) Synthesis of catechol-modified chitosan
[0056] Disperse 0.5g of chitosan (CS) in 100mL of pH 5.5 aqueous solution, stir for 12h until completely dissolved to form chitosan aqueous solution; take 1.092g of dihydrocaffeic acid (HCA) and 0.581g of 1-ethyl-(3- Dimethylaminopropyl) carbodiimide hydrochloride (EDC·HCl) is dissolved in a mixed solvent of ethanol and water to form a dihydrocaffeic acid HCA solution, and then the HCA solution is added dropwise to the chitosan In sugar aqueous solution; adjust the pH of the mixed reaction solution to 5.5, and react in an ice-water bath for 24 hours; finally, dialyze the reaction solution against acidic water for 24 hours, freeze-dry to obtain dihydrocaffeic acid modified chitosan CS-HCA, and store it in the dark for future use ;
[0057] (2) Preparation of modified chitosan-gold hybrid nanopar...
Embodiment 3
[0067] A preparation method of an immunosensor based on chitosan-gold hybrid particles, comprising the following specific steps:
[0068] (1) Synthesis of catechol-modified chitosan
[0069] Disperse 1.0g chitosan (CS) in 100mL pH 4.5 aqueous solution, stir for 12h until completely dissolved to form chitosan aqueous solution; take 4.368g caffeic acid (CAA) and 1.162g 1-ethyl-(3-dimethyl Aminopropyl) carbodiimide hydrochloride (EDC·HCl) is dissolved in the mixed solvent of ethanol and water, forms caffeic acid CAA solution, then CAA solution is added dropwise in the described chitosan aqueous solution; Adjust the pH of the mixed reaction solution to 5.5, and react in an ice-water bath for 24 hours; finally, the reaction solution was dialyzed against acidic water for 48 hours, freeze-dried to obtain caffeic acid-modified chitosan CS-CAA, and stored in the dark for future use;
[0070] (2) Preparation of modified chitosan-gold hybrid nanoparticles
[0071] Dissolve 2 mg of caff...
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