Preparation method of immunosensor based on chitosan-gold hybrid particles

An immunosensor, chitosan technology, applied in the field of preparing electrochemical immunosensors to achieve the effects of improving electrical conductivity, excellent stability and biocompatibility, and improving long-term adhesion

Inactive Publication Date: 2018-04-27
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the preparation of immunosensors based on polymer-inorganic hybrid nanoparticles has not been reported so far.

Method used

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  • Preparation method of immunosensor based on chitosan-gold hybrid particles
  • Preparation method of immunosensor based on chitosan-gold hybrid particles
  • Preparation method of immunosensor based on chitosan-gold hybrid particles

Examples

Experimental program
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Effect test

Embodiment 1

[0041] A preparation method of an immunosensor based on chitosan-gold hybrid particles, comprising the following specific steps:

[0042] (1) Synthesis of catechol-modified chitosan

[0043] Disperse 1.0g chitosan (CS) in 100mL pH 4.0 aqueous solution, stir for 12h until completely dissolved to form chitosan aqueous solution; take 2.183g caffeic acid (CAA) and 1.162g 1-ethyl-(3-di Methylaminopropyl) carbodiimide hydrochloride (EDC·HCl) was dissolved in a mixed solvent of ethanol and water to form a caffeic acid solution, and then the catechol derivative solution was added dropwise to the chitosan sugar aqueous solution; adjust the pH of the mixed reaction solution to 5.5, and react in an ice-water bath for 12 hours; finally, the reaction solution was dialyzed against acidic water for 12 hours, freeze-dried to obtain caffeic acid-modified chitosan CS-CAA, and stored in the dark for future use;

[0044] (2) Preparation of modified chitosan-gold hybrid nanoparticles

[0045] Sc...

Embodiment 2

[0054] A preparation method of an immunosensor based on chitosan-gold hybrid particles, comprising the following specific steps:

[0055] (1) Synthesis of catechol-modified chitosan

[0056] Disperse 0.5g of chitosan (CS) in 100mL of pH 5.5 aqueous solution, stir for 12h until completely dissolved to form chitosan aqueous solution; take 1.092g of dihydrocaffeic acid (HCA) and 0.581g of 1-ethyl-(3- Dimethylaminopropyl) carbodiimide hydrochloride (EDC·HCl) is dissolved in a mixed solvent of ethanol and water to form a dihydrocaffeic acid HCA solution, and then the HCA solution is added dropwise to the chitosan In sugar aqueous solution; adjust the pH of the mixed reaction solution to 5.5, and react in an ice-water bath for 24 hours; finally, dialyze the reaction solution against acidic water for 24 hours, freeze-dry to obtain dihydrocaffeic acid modified chitosan CS-HCA, and store it in the dark for future use ;

[0057] (2) Preparation of modified chitosan-gold hybrid nanopar...

Embodiment 3

[0067] A preparation method of an immunosensor based on chitosan-gold hybrid particles, comprising the following specific steps:

[0068] (1) Synthesis of catechol-modified chitosan

[0069] Disperse 1.0g chitosan (CS) in 100mL pH 4.5 aqueous solution, stir for 12h until completely dissolved to form chitosan aqueous solution; take 4.368g caffeic acid (CAA) and 1.162g 1-ethyl-(3-dimethyl Aminopropyl) carbodiimide hydrochloride (EDC·HCl) is dissolved in the mixed solvent of ethanol and water, forms caffeic acid CAA solution, then CAA solution is added dropwise in the described chitosan aqueous solution; Adjust the pH of the mixed reaction solution to 5.5, and react in an ice-water bath for 24 hours; finally, the reaction solution was dialyzed against acidic water for 48 hours, freeze-dried to obtain caffeic acid-modified chitosan CS-CAA, and stored in the dark for future use;

[0070] (2) Preparation of modified chitosan-gold hybrid nanoparticles

[0071] Dissolve 2 mg of caff...

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Abstract

The invention discloses a preparation method of an immunosensor based on chitosan-gold hybrid particles. The preparation method comprises the following steps: catechol group-modified chitosan synthesis, preparation of modified chitosan-gold hybrid nanoparticles, and construction of the immunosensor. The hybrid nanoparticles have good biological compatibility, adhesiveness and conductivity, an application of the polymer-inorganic conductive hybrid nanoparticles in the immunosensor can effectively increase a fixed amount of an antibody and can better keep the activity of biomacromolecules, adhesive force and conductivity of a sensing coating layer can be effectively enhanced, so that the constructed immunosensor has the advantages of high specificity, good long-term stability, wide detectionscope, and low detection lower limit; a nanocomposite material and an electrochemical sensor are combined, and the immunosensor can be widely used for immunization analysis and development in the fields of food safety, biological medicine and environmental protection monitoring.

Description

technical field [0001] The invention relates to the fields of composite nanomaterials, immunoassays and electrochemical sensors, in particular to an electrode modified by hybrid nanoparticle-catechol group-modified chitosan@gold composite nanoparticle, and prepared as Electrochemical immunosensor approach. Background technique [0002] As we all know, cancer is a major killer of human health. Clinically, the detection of tumor markers such as alpha-fetoprotein (AFP), carbohydrate antigens (CA125, CA19-9), carcinoembryonic antigen (CEA), etc., can be used for the detection of colon cancer, lung cancer, pancreatic cancer, gastric cancer and other glandular epithelial malignancies. It has important reference value for judging the curative effect, disease development, monitoring and prognosis estimation of tumors and other cancers. At present, there are many immunoassay methods for detecting tumor markers in serum, such as chemiluminescence immunoassay, fluorescence immunoassa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543G01N27/327
CPCG01N33/54346G01N27/3278
Inventor 刘晓亚许升吴倩黄雪雯赵伟朱叶罗静朱海燕
Owner JIANGNAN UNIV
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