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Method for preparing electrochemiluminescence biological sensing interface based on loaded graphite phase carbon nitride and application thereof

A graphite-phase carbon nitride and sensing interface technology, applied in chemiluminescence/bioluminescence, analysis through chemical reactions of materials, biological testing, etc., can solve problems such as poor reproducibility and complicated electrode modification steps, and achieve Easy to operate, simple and fast fixation, high luminous stability effect

Active Publication Date: 2015-06-03
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] One of the purposes of the present invention is to provide a simple and easy method for preparing a label-free electrochemiluminescence sensing interface to solve the problems of complicated electrode modification steps and poor reproducibility

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0024] (1) Preparation of supported graphite phase carbon nitride: the gold-silver alloy film with a gold-silver mass ratio of 1:1 was corroded with concentrated nitric acid to prepare nanoporous gold with a three-dimensional double continuous porous structure, which was dispersed in ethanol; 5 g of melamine was subjected to high temperature polycondensation at 550°C to prepare graphitic carbon nitride, and then 100 mg of graphitic carbon nitride was ultrasonically stripped in 100 mL of ultrapure water for 24 hours, and centrifuged at 5000 rpm to remove unexfoliated graphite carbon nitride , to prepare an aqueous solution of graphitic carbon nitride nanosheets; 1 mL ethanol dispersion of nanoporous gold (1.5 mg?mL -1 ) with 1 mL of graphitic carbon nitride nanoplatelet aqueous solution (0.4 mg?mL -1 ) mixed, shaken for 2 hours, centrifuged to remove the supernatant, added 1 mL of ultrapure water, and ultrasonicated for 2 hours to prepare a nanocomposite solution of supported g...

Embodiment 2

[0030] (1) Preparation of supported graphite phase carbon nitride: the gold-silver alloy film with a gold-silver mass ratio of 1:1 was corroded with concentrated nitric acid to prepare nanoporous gold with a three-dimensional double continuous porous structure, which was dispersed in ethanol; 5 g of melamine was subjected to high temperature polycondensation at 550°C to prepare graphitic carbon nitride, and then 100 mg of graphitic carbon nitride was ultrasonically stripped in 100 mL of ultrapure water for 24 hours, and centrifuged at 5000 rpm to remove unexfoliated graphite carbon nitride , to prepare an aqueous solution of graphitic carbon nitride nanosheets; 1 mL ethanol dispersion of nanoporous gold (2.0 mg?mL -1 ) with 1 mL of graphitic carbon nitride nanoplatelet aqueous solution (0.6 mg?mL -1 ) mixed, shaken for 2 hours, centrifuged to remove the supernatant, added 1 mL of ultrapure water, and ultrasonicated for 3 hours to prepare a nanocomposite solution of supported g...

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Abstract

The invention discloses a method for preparing an electrochemiluminescence biological sensing interface based on loaded graphite phase carbon nitride and an application thereof, and relates to the field of nano science, biological immunoassay, electrochemical sensing, etc. By means of the excellent electrochemiluminescence of a graphite phase carbon nitride nanosheet and the unique three-dimensional porous structure and good biocompatibility of nano porous gold, the graphite phase carbon nitride nanosheet is loaded in a nano porous gold skeleton for constructing a marker-free electrochemiluminescence biological sensing interface. Due to the three-dimensional porous structure, the luminescence property of the graphite phase carbon nitride nanosheet is greatly improved, and meanwhile, a biological recognition unit is fixed simply and quickly due to the good biocompatibility of the nano porous gold. The method is simple in steps and easy to operate, has relatively high luminescence stability and good response performance, and solves the problems of complicated steps and poor signal reproducibility of conventional methods. The method can be applicable to the preparation of a variety of electrochemiluminescence biological sensing interfaces of biomarkers, and has a wide application prospect in scientific research and clinical practice.

Description

technical field [0001] The invention relates to the fields of nanoscience, biological immunity technology, electrochemical sensing and the like, and in particular to a preparation method and application of an electrochemical luminescence biosensing interface based on loaded graphite phase carbon nitride. Background technique [0002] Biomarker detection is currently the only non-invasive method for early warning of cancer and other diseases. It is of great significance to the general screening, diagnosis and prognosis of tumors in clinical practice. Among the many biomarker detection methods, electrochemiluminescence immunoassay is currently the most advanced immunoassay technology, which has the advantages of simple operation, small sample volume, fast sensitivity, low cost and easy miniaturization. It is widely used in clinical and scientific research. Has been widely used. [0003] Early electrochemiluminescent immunosensors usually detect signals by labeling biomolecule...

Claims

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Application Information

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IPC IPC(8): G01N21/76G01N33/68
Inventor 马洪敏魏琴王欢李小建张森张勇吴丹
Owner UNIV OF JINAN
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