Recombinant corynebacterium glutamicum capable of being used for highly yielding L-phenylalanine and application thereof

Abstract

The invention discloses recombinant corynebacterium glutamicum capable of being used for highly yielding L-phenylalanine and a method for producing the L-phenylalanine (Phe) by fermentation and belongs to the field of metabolic engineering. According to the invention, a corynebacterium glutamicum engineering strain C.glutamicum19AF / 99TP capable of being used for highly yielding the L-Phe is obtained in a corynebacterium glutamicum type strain ATCC13032 by carrying out induction expression on the following four genes: a 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase gene, a chorismate mutase / prephenate dehydratase gene which has resistance to feedback inhibition, a transketolase gene and a phosphoenolpyruvic acid synthetase gene. A shikimic acid metabolic pathway of an L-Phe synthetic metabolic pathway is over-expressed in the C.glutamicum ATCC13032; a key enzyme gene of a chorismic acid metabolic pathway enables the yield of the L-Phe to reach 3.47g / L; two enzyme genes which combines an expression center metabolic pathway to improve a precursor enables the highest yield of the L-Phe to reach 4.86g / L; and the original strain ATCC13032 which is used as a contrast strain cannot detect accumulation of the L-Phe in the integral fermenting process.

Description

technical field [0001] The invention relates to a recombinant Corynebacterium glutamicum with high production of L-phenylalanine and application thereof, in particular to a metabolically modified recombinant Corynebacterium glutamicum and a method for producing L-phenylalanine. Background technique [0002] Phenylalanine (Phenylalanine), namely D, L-α-amino-β-phenylpropionic acid, has three racemic DL-type, L-type and D-type, which has biologically active optical isomerism The body is L-phenylalanine (L-Phenylalanine, L-Phe), and the specific rotation is -35.1°. L-Phe, also known as L-phenyl-alanine, is a white or colorless crystalline powder solid at room temperature. The solubility in water is 29.6g / L, and it is insoluble in methanol, ethanol and ether. L-Phe widely exists in eggs, milk and animal protein in nature, with a content of 5%-6%; L-Phe also exists in plant protein, with a content of about 1%. [0003] L-Phe is one of the eight essential amino acids that cannot...

AI Technical Summary

Problems solved by technology

In recent years, due to the high cost and limited sources of main raw materials such as substrates and enzymes, and the poor stability of enzymes in the reaction process, the enzymatic production of L-Phe has also been greatly restricted.

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