99results about How to "Clear background" patented technology

A non-continuous immunoassay device which includes two or more separated pads for immunoassay analysis, and is capable of controlling the migration speed of a mobile phase between the separated pads, and an immunoassay method using the same are disclosed. The immunoassay device includes a first pad receiving a mobile phase; a second pad which is spatially separated from the first pad by a predetermined distance, and to which the mobile phase migrates; an upper case for covering the upper parts of the first pad and the second pad; a lower case for covering the lower parts of the first pad and the second pad; and a connecting member which is formed on at least one of the upper case and the lower case, and located between the first pad and the second pad to form a passage for moving the liquid sample.

The invention discloses a preservation solution for exfoliated cell and a preparation method which uses the preservation solution to process cell thin layer smear. The preservation solution for exfoliated cell comprises the components which are prepared by the volume proportion of 10 to 15 portions of methanol, 10 to 15 portions of alcohol, 1 to 2 portions of glycerin, 1 to 2 portions of glycol polyethylene, 10 to 12 portions of formaldehyde, 0.5 to 1 portions of glacial acetic acid, 10 to 20 portions of sodium chloride with 0.85 percent to 0.9 percent of mass concentration and 33 percent to 68.5 percent of phosphate buffer. The steps of the preparation method for cell thin layer smear are that: 1) the collected sample is put in the preservation solution for exfoliated cell for shaking; the sample is transferred to a centrifuge for centrifugal treatment after standing; 2) the supernatant is removed until 1ml to 2ml residue is left in the centrifuge tube; the cell suspension is obtained after uniform vibration; 3) 0.5ml to 1ml cell suspension is put into a smear preparation cup which is made into cell thin layer smear by a cell smear preparation machine.

The invention provides an economical, simple, easily operated and rapid extraction and dimensional electrophoresis method of mangrove plant total protein. Repeated experiments have proved that a sample prepared by the method has good repeatability and an intelligible spectrum, and the technical scheme is especially suitable for an extraction and dimensional electrophoresis method of mangrove plant total protein. The invention employs 20% TCA-acetone solution to extract protein; 20% TCA can better remove salt ion, and acetone with a concentration of 80% has substantial effect on separating of a protein and condensed tannin compound, and can better separate the compound, remove tannin and increase protein concentration; the mangrove plant protein extracted by 20% TCA-acetone has high content and an intelligible 2-DE electrophoresis pattern; besides, the method is simply operated, with good repeatability and low reagent toxicity.

The invention belongs to the field of cell treating fluid, and particularly relates to alkali solution based cell preservation treating fluid and a preparation method thereof. The alkali solution based cell preservation treating fluid is mainly composed of an egg white dissolution agent, a red blood cell cracking agent, an alkaline substance, a corrosion remover and distilled water. The alkali solution based cell preservation treating fluid is mainly used in cooperation with a membrane type liquid based thin-layer cell slide preparation machine and can enhance the cell adhesion capability of a modified glass slide, promote cervical cell enrichment so as to firmly attach cervical cells to the specially-prepared glass slide and effectively reduce the phenomenon of leak detection. Meanwhile, the novel alkali solution based cell preservation treating fluid has the advantages of being free of toxicity, environmentally friendly, capable of rapidly and thoroughly achieving sterilization, low in cost, convenient to configure, safe and efficient.

The invention discloses an extraction method for mangrove plant kandelia candel leaf total protein suitable for two-dimensional electrophoresis. A traditional trichloroacetic acid/acetone precipitation method for protein extraction and a traditional phenol extraction method for protein extraction are combined, a cosolvent SDS solution is introduced, the extraction process is optimized, and the Phe-B method suitable for extraction of the mangrove plant kandelia candel leaf total protein is established. The extraction efficiency and quality of the protein are effectively improved, and the technical scheme can be specifically suitable for extraction of the kandelia candel leaf protein in two-dimensional electrophoresis. The method has the advantages of being easy to operate, high in protein extraction efficiency and fewer in interfering substance, and is suitable for materials with difficult protein extraction, low protein extraction efficiency and more interfering substances. The extracted kandelia candel leaf protein completely meets the requirements of the first direction and the second direction of two-dimensional electrophoresis, a high-quality two-dimensional electrophoresis gel map with high resolution, more clear protein points, uniform distribution and the clear background can be obtained, and experiment repeatability and stability are good.

The invention relates to a mouse anti-human CEA (Carcino-Embryonic Antigen) monoclonal antibody and a hybridoma cell strain secreting the same. The clone number of the hybridoma cell strain secreting the CEA monoclonal antibody is 10E1, and the collection number of the hybridoma cell strain is CGMCC No. 6911. The invention further provides the monoclonal antibody generated by the hybridoma cell strain 10E1. The antibody comprises a heavy-chain variable region and a light-chain variable region, wherein an amino acid sequence of the heavy-chain variable region is SEQ ID NO: 9, and an amino acid sequence of the light-chain variable region is SEQ ID NO: 10. The invention further provides a DNA (Deoxyribonucleic Acid) molecule of SEQ ID NO: 7 and a DNA molecule of SEQ ID NO: 8, wherein the amino acid sequence, namely SEQ ID NO: 9, of the heavy-chain variable region is coded by the DNA molecule of SEQ ID NO: 7, and the amino acid sequence, namely SEQ ID NO: 10, of the light-chain variable region is coded by the DNA molecule of SEQ ID NO: 8. The antibody can be applied to scientific research or the clinical immunohistochemical detection of CEA expression.

The present invention discloses a medical test paper strip for a fast diagnosis of schistosomiasis japonicum in domestic animals. Said medical test paper strip in accordance with the present invention adopts SEA antibody and SEA coated nitrocellulose membrane as a quality control line and a detection line and is manufactured using a double antigen sandwich method, wherein the SEA antigen is labelled by colloidal gold. The present invention has a remarkable specificity and a high sensitivity, and is operated conveniently, simply and quickly. The present invention has a low manufacturing cost and then is suitable for a mass production.

The invention discloses a method for simultaneously labelling collagen type IV, macrophage and neovascularisation of tumors. The method comprises the following steps: 1. immobilizing tissue sections on a plus microscope slide processed by polylysine; 2. dewaxing the tissue sections in dimethylbenzene; 3. carrying out antigen retrieval; 4. washing the sections; 5. carrying out confining; 6. addingprimary antibodies; 7. washing the sections; 8. carrying out confining in the same way as the step 5; 9. using the fragment antigen-binding F(ab')2-QDs525 of goat anti-mouse immunoglobulin G labelledby quantum dot QDs525, the fragment antigen-binding F(ab')2-QDs585 of goat anti-rabbit immunoglobulin G labelled by quantum dot QDs585 and the fragment antigen-binding F(ab')2-QDs655 of rabbit anti-goat immunoglobulin G labelled by quantum dot QDs655 as secondary antibodies and dropwise adding the mixture after removing the confining liquid; 10. washing the sections; and 11. sealing the sections:preparing buffered glycerol with glycerol and 10ml of tris buffered saline (TBS) and storing the sections after sealing the sections with the buffered glycerol. The method is used for efficiently, accurately, rapidly and simultaneously detecting various components in tumor tissue microenvironment.

A 3d image photographing device is disclosed for producing a 3D image with enhanced stereoscopic effect. The device includes a first track, a second track, a slide carriage and a driving module. The first track couples with the second track. The first track includes a first slideway in an arc shape. The second track includes a second slideway in an arc shape, and a curvature of the second slideway is larger than a curvature of the first slideway. The slide carriage includes a base. A first sliding block is rotatably mounted to a surface of the base, and a groove is arranged on the surface. The first sliding block movably coupled to the second slideway. A positioning member couples with the groove, with the positioning member having a second sliding block movably coupled to the first slideway, and with the second sliding block connecting with a blocking portion received in the groove. A coupling portion is mounted to another surface of the base for coupling a camera.

The invention discloses a liquid-based cell sheet producing system which comprises a rack, a sheet producing platform fixed on the rack, a plurality of sheet producing plates fixed on the sheet producing platform, a moving device perpendicularly connected with a bearing frame of the rack, a mechanical arm perpendicularly and slidingly connected with the moving device, a cantilever perpendicularly and slidingly connected with the mechanical arm, a reagent bottle connected with the cantilever by a catheter, and a microcomputer system controlling the moving device, the mechanical arm and the cantilever, wherein a control window is arranged on the rack, and controls the microcomputer system; and the reagent bottle comprises a waste liquid barrel, a flushing fluid reagent bottle part and a buffer fluid reagent bottle part. A liquid-based cell sheet producing method adopts the liquid-based cell sheet producing system. With the adoption of the technical scheme, the defects that a system operation mode is single, a procedure is solidified, a pipeline cannot be flushed automatically, a requirement on the quantity of specimens is strict, flux is low, energy consumption and reagent consumption material loss are great, a failure rate is high, maintenance is difficult, and equipment is costly are overcome.

The invention relates to a high-temperature Daqu leaching liquor macro-protein extraction and purification method. The method comprises the following steps: crushing high-temperature Daqu, spraying sterile water and evenly mixing, storing in a biological culture tank, performing activated culturing for later use by adopting a stage temperature rise method; adding an acetic acid-sodium acetate buffer solution and a phenylmethylsulfonyl fluoride solution, shaking and blending evenly, and then leaching overnight, filtering and centrifuging to obtain high-temperature Daqu leaching liquor; sequentially adding a TCA acetone solution, an ammonium acetate methanol solution, an acetone solution, a Tris satured phenol/lauryl sodium sulfate buffer solution, an ammonium acetate methanol solution, and a methanol and acetone solution to obtain sediment A, sediment B, phenol layer, sediment C, sediment D and high-temperature Daqu macro-protein samples; adding a sample lysate, performing ice bath ultrasonic for hydrotropy, and centrifuging the sediments to obtain a high-temperature Daqu macro-protein sample solution. The high-temperature Daqu macro-protein sample solution prepared by adopting the method can be applied to two-dimensional electrophoresis of the high-temperature Daqu macro-protein, to obtain high-resolution and high-repeatability two-dimensional electrophoretogram.

The invention relates to a method for preparing a pulled type cervical smear. The method comprises the following steps: (1) oscillating a specimen through an oscillator; (2) centrifugating a part of the oscillated specimen until the specimen is layered into supernatant liquid and a cell layer; (3) removing the supernatant liquid, sucking the cell layer, dripping the cell layer on a glass slide, laminating the glass slide with another glass slide, and then pulling the glass slides to the opposite directions; (4) fixing and dyeing so as to prepare the smear. Compared with a conventional Pap smear method, the method provided by the invention has the advantages of high specimen satisfaction, high smear quality and the like. Compared with a liquid base cytology method, the method provided by the invention has the advantages of simplicity in operation, uniform tiling of the prepared smears, non-overlapping of cells, clear background, no precious equipment, low cost and the like, and is convenient to popularize. Compared with a human papillomavirus-deoxyribose nucleic acid (HPV-DNA) detection method, the method provided by the invention has the advantages of no use of precious equipment, low inspection cost, simplicity in operation, convenience in popularization and the like.

The invention discloses a bone marrow cell culture medium. Antibiotics and solid serum substitutes are added to a basal culture medium. The bone marrow cells cultured by the culture medium provided bythe invention can be used for chromosome preparation, and the culture medium shows the advantages of good chromosome presentation state, easiness in observation and the like, and has wide applicationprospect in the field of medical detection.

The invention discloses recombinant saccharomyces cerevisiae for producing gastrodin by using glucose and application of the recombinant saccharomyces cerevisiae. The construction method of the engineering strain comprises the step of enabling recipient bacteria to express UDP-glucosyltransferase derived from hedyotis diffusa. The recombinant saccharomyces cerevisiae does not express an ARO7 gene, contains genes AsUGTsyn, CARsyn, PPTcg-1syn and ubiCsyn capable of expressing the gastrodin synthesis pathway and genes ppsA, tktA, ARO1, ARO2 and ARO4 mutant ARO4K229L genes capable of expressing gastrodin precursor synthesis enhancement. According to the application, a metabolic pathway for synthesizing gastrodin from glucose is constructed in food-grade saccharomyces cerevisiae by introducing new glycosyl transferase, the fermentation yield of gastrodin in a 250mL shake flask reaches 2.1 g/L through a genome integration technology and improvement of a precursor anabolic flow, a foundation is laid for large-scale industrial production, and important economic values and social benefits are achieved.

The invention discloses a method for extracting animal mitochondria DNA, which has the innovativeness that a DNasel digestion step is added on the basis of alkaline denaturation to eliminate residual nuclear DNA; and an RNase digestion step is added to remove residual RNA. The mitochondria DNA extracted by the method has high purity. The electrophoretic detection shows that an electrophoretic band is a clear, regular and uniform band; and the background is clear, and the conditions such as the trailing of macromolecular DNA and front-end RNA and the like do not occur near a sample application pore.

The invention discloses a method for extracting and purifying a columnar high-purity animal mtDNA. In the method, an alkaline denaturation method is taken as a basis and improved by adding a DNaseI digestion step to eliminate the residue of nuclear DNA, and by adding a RNase digestion step to eliminate the residue of RNA; a column chromatography is adopted to replace a phenol extraction method for purifying the mtDNA to solve the problems of phenol toxicity and residue thereof. The mitochondrial DNA obtained by the method has high purity; and electrophoresis detection displays that an electrophoresis band is clear, neat and even with clear background and without the situations of macromolecular DNAs near sample application holes, front-end DNA trailing, and the like.

The invention discloses a two-dimensional electrophoresis method for separating protein from a kenaf leaf efficiently and stably. In combination of biomass chemical features of the kenaf leaf, on the basis of the conventional protein two-dimensional electrophoresis method, the method for separating protein from the kenaf leaf, the selection of the pH range of an IPG gel strip, the protein sample applying way, the protein sample applying amount, the concentration of a separation gel, the setting of parameters of protein spot analyzing software and other aspects are improved and optimized. By the method, a two-dimensional electrophoretogram of the protein of the kenaf leaf with more clear protein spots which are uniformly distributed and with clear background can be obtained; the experimental result is stable; and averagely, 1300 protein spots can be detected on the gel with the size of 24cm*16cm. The invention paves a working foundation for further proteomic and molecular biologic research on kenaf and other hemps.

The invention belongs to the technical field of veterinary biological products, and particularly relates to a preparation method of a duck reovirus inactivated vaccine. In the invention, an LMH passage cell line is used as a carrier cell for virus proliferation; a culture medium A consisting of lysine, recombinant human insulin, human serum albumin, protamine, biotin, polysaccharide sulfate and agrowth factor is used for culture; and the virus fluid is collected and inactivated to prepare a vaccine. The LMH passage cell line is used as a carrier cell for virus proliferation without the need of adding pancreatin, and the LMH cells have a clear background, have no exogenous pathogens and are easy for proliferation, which can effectively simplify the process and reduce costs. Meanwhile, theduck reovirus inactivated vaccine prepared by the invention has high virus content, good stability and high safety, and is an ideal duck reovirus inactivated vaccine.

The invention belongs to the technical field of buchloe dactyloides molecular breeding, and particularly relates to an optimal buchloe dactyloides ISSR-PCR (inter-simple sequence repeat-polymerase chain reaction) reaction system. The invention provides the following ISSR-PCR reaction system that: each 25muL of reaction system contains 1*PCR buffer solution, 0.3mM of dNTPs, 1.0U of Taq enzyme, 0.6muM of any one of primers shown as SEQ ID 1-7, 2.0mM of Mg2+ and 50ng of DNA template; and the reaction mixed solution is amplified by the following procedures of: pre-denaturing for 3 minutes at 94 DEG C, denaturing for 30 seconds at 94 DEG C, annealing for 30 seconds at 50 to 60 DEG C, stretching for 2 minutes at 72 DEG C, circulating for 35 times, and stretching for 4 minutes at 72 DEG C. The buchloe dactyloides ISSR-PCR provided by the invention has good stability and high definition, and increases the richness of strips.