Wheat seed purity identification method

A technology for identification of wheat seeds and purity, applied in the direction of material electrochemical variables, etc.

Inactive Publication Date: 2015-10-21
SHANDONG CROP GERMPLASM CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are also the following problems in this method: the drug concentration ratio is not accurate enough, the experimental procedures are cumbersome, resulting in poor operability of the technical procedure, the electrophoretic medium gel produced has sufficient toughness but insufficient strength, and individual drugs are highly toxic and difficult to obtain. Procurement, which greatly affects the efficiency and success rate of detection
[0004] Patent No. CN201310325497 titled "a method for identifying the purity of corn seeds" The method described in the invention patent cannot be used to identify the purity of wheat, and what is identified by corn seeds is Salt-soluble protein, which is specific among maize varieties but not different among wheat varieties, so it cannot be used to identify the purity of wheat
The wheat purity identification method identifies the prolamin components in wheat, which have unique heredity, stability and diversity in wheat varieties, and can correctly distinguish the differences between wheat varieties, so the method for identifying the purity of corn seeds cannot be used for wheat Variety identification

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] 1. Solution preparation

[0066] (1) Electrode buffer

[0067] Weigh 0.4 g of glycine, add distilled water to dissolve, add 4 mL of glacial acetic acid, add distilled water to make the volume to 1000 mL, and mix well.

[0068] (2) Sample extract

[0069] Weigh 0.05 g of methyl green, dissolve in 100 mL of 70% (wt) ethanol, and store at 4°C. This extract is essentially a protein extract.

[0070] (3) Gel solution

[0071] Weigh 150.0g of acrylic amine, 15.0g of N,N'-methylenebisacrylamide, 60.0g of urea, 1.0g of ascorbic acid, 1.0g of glycine, 0.05g of ferrous sulfate, add distilled water to dissolve, add 20 mL of glacial acetic acid, Dilute to 1000mL and store at 4°C.

[0072] (4) 0.6% (wt) hydrogen peroxide solution

[0073] Take 2 mL of 30% (wt) hydrogen peroxide, add distilled water to make up to 100 mL, and store at 4°C.

[0074] (5) Dyeing solution

[0075] Weigh 0.25 g of Coomassie Brilliant Blue (R250), add 25 mL of absolute ethanol to dissolve, add 50 g o...

Embodiment 2

[0094] Example 2 Comparative example

[0095] Different varieties of wheat seeds Jinan 17, Jimai 19, Jimai 20, Jimai 21, Weimai No. 8 are identified, respectively according to the method in Example 1 and the National Standard of the People's Republic of China "Crop Seed Inspection Regulations GB / T3543 .5-1995" method for identification, the results correspond to figure 1 , figure 2 .

[0096] Compare figure 1 , figure 2 , comparing the spectra before and after the improvement, the results show that: figure 1 After the electrophoresis technology is improved, the protein band boundaries are more obvious, the characteristic bands and common bands are clearer and more stable, the resolution is greatly improved, and the implementation effect is good.

Embodiment 3

[0097] Embodiment 3 Comparative example

[0098] According to the method in Example 1, the wheat variety "Jimai 22" was electrophoresed with different extractants. The sample extracts were 70% (wt) ethanol and 25% (wt) α-chloroethanol. image 3 , Figure 4 .

[0099] By comparing the electrophoretic effects of the substitute ethanol and the original extractant, it can be seen that there is no difference between the two maps.

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Abstract

The invention discloses a wheat seed purity identification method which is developed on the basis of conventional methods. The identification method has the following improvements: (1) nontoxic and harmless ethanol is used to replace alpha-chloroethanol as an extraction liquid, and thus the harm to human body and pollution to environment are both effectively reduced; (2) the acrylamide concentration and crosslinking degree are properly adjusted, thus the film becomes tough and elastic, and the functions of molecular sieve can be better exerted; (3) the gel can be filled in one step, and the steps are saved. The provided identification method has the characteristics of concise technology process, simple operation, short time, and environment-friendliness. Moreover, a novel film washing and preservation technology is adopted, the background of the washed film is clear, the film can be preserved for a long time after the film is soaked in a preservation liquid and sealed by a preservative film, and thus the film can be applied to the post identification and research.

Description

technical field [0001] The invention relates to an improved method for identifying the purity of wheat seeds, which is specifically applied to the fields of rapid identification of wheat seed purity and identification of germplasm resources. Background technique [0002] Wheat is the most widely used crop in my country. Seed purity is one of the important indicators of seed quality and the fundamental basis for seed classification and evaluation of seed quality. Seed purity inspection can prevent mixed degeneration of seeds in production, improve seed quality, maintain good variety, and continuously increase crop yield. [0003] The identification technology of seed variety purity can be divided into four categories according to the means of detection: morphological markers, physical and chemical characteristics identification, physiological and biochemical identification, molecular biological identification, etc. Wheat seed purity identification technology is one of the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26
Inventor 李群颜廷进丁汉凤田茜戴双张文兰段乃彬
Owner SHANDONG CROP GERMPLASM CENT
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