Alkali solution based cell preservation treating fluid and preparation method thereof

Abstract

The invention belongs to the field of cell treating fluid, and particularly relates to alkali solution based cell preservation treating fluid and a preparation method thereof. The alkali solution based cell preservation treating fluid is mainly composed of an egg white dissolution agent, a red blood cell cracking agent, an alkaline substance, a corrosion remover and distilled water. The alkali solution based cell preservation treating fluid is mainly used in cooperation with a membrane type liquid based thin-layer cell slide preparation machine and can enhance the cell adhesion capability of a modified glass slide, promote cervical cell enrichment so as to firmly attach cervical cells to the specially-prepared glass slide and effectively reduce the phenomenon of leak detection. Meanwhile, the novel alkali solution based cell preservation treating fluid has the advantages of being free of toxicity, environmentally friendly, capable of rapidly and thoroughly achieving sterilization, low in cost, convenient to configure, safe and efficient.

Description

technical field

[0001] The invention belongs to the field of cell treatment liquid, and in particular relates to an alkaline liquid-based cell preservation treatment liquid and a preparation method thereof. Background technique

[0002] Cervical cancer is one of the most common malignant tumors among female cancers. Early diagnosis of cervical cancer and precancerous lesions is the key to improving the cure rate of cervical cancer and the survival rate of patients. Cytological examination is currently the most widely used clinical method for screening cervical cancer. In recent years, cytological examination has become the main method for large-scale cervical cancer screening and follow-up observation of high-risk groups, which has greatly reduced the incidence of advanced cervical cancer.

[0003] The traditional method of cytological examination is to use the Pap smear method. The obtained smears have a large number of overlapping cells, and the smears also contain a larg...

Examples

Embodiment 1

[0038] Embodiment 1, an alkaline liquid-based cell preservation treatment solution

[0039] The alkaline liquid-based cell preservation treatment solution consists of the following components and their weight percentages:

[0040] Protein dissolving agent 0.4%, erythrocyte lysing agent 0.01%, NaOH 0.02%, Kathon 0.05% and distilled water 99.52%; Described protein dissolving agent is made up of N-acetyl-L cysteine, urea and dithiothreitol according to The weight ratio is 4:5:3; the erythrocyte lysing agent is composed of saponin, sodium lauryl sulfate and polyethylene glycol octylphenyl ether in a weight ratio of 3:4:4.

[0041] Preparation:

[0042] Mix protein dissolving agent, erythrocyte lysing agent, alkaline substances and preservatives, add appropriate amount of distilled water and stir until completely dissolved, then add distilled water until the pH value is 8, and the product is obtained.

Embodiment 2

[0043] Embodiment 2, an alkaline liquid-based cell preservation treatment solution

[0044] The alkaline liquid-based cell preservation treatment solution consists of the following components and their weight percentages:

[0045] Protein dissolving agent 0.6%, erythrocyte lysing agent 0.05%, NaOH 0.012%, chlorhexidine acetate 0.18% and distilled water 99.158%; Said protein dissolving agent consists of N-acetyl-L cysteine, urea and dithiothreose The composition of alcohol is 5:4:2 by weight; the erythrocyte lysing agent is composed of saponin, sodium lauryl sulfate and polyethylene glycol octyl phenyl ether by weight ratio of 4:3:3.

[0046] Preparation:

[0047] Mix protein dissolving agent, erythrocyte lysing agent, alkaline substances and preservatives, add appropriate amount of distilled water and stir until completely dissolved, then add distilled water until the pH value is 8.6.

Embodiment 3

[0048] Embodiment 3, an alkaline liquid-based cell preservation treatment solution

[0049] The alkaline liquid-based cell preservation treatment solution consists of the following components and their weight percentages:

[0050] Protein dissolving agent 1%, erythrocyte lysing agent 0.1%, KOH 0.001%, domiphene 0.02% and distilled water 98.879%; said protein dissolving agent consists of N-acetyl-L cysteine, urea and dithiothreitol Composed by weight ratio of 6:3:1; the erythrocyte lysing agent is composed of saponin, sodium lauryl sulfate and polyethylene glycol octylphenyl ether in a weight ratio of 5:2:2.

[0051] Preparation:

[0052] Mix protein dissolving agent, erythrocyte lysing agent, alkaline substances and preservatives, add appropriate amount of distilled water and stir until completely dissolved, then add distilled water until the pH value is 9, and the product is obtained.

technical field

[0001] The invention belongs to the field of cell treatment liquid, and in particular relates to an alkaline liquid-based cell preservation treatment liquid and a preparation method thereof. Background technique

[0002] Cervical cancer is one of the most common malignant tumors among female cancers. Early diagnosis of cervical cancer and precancerous lesions is the key to improving the cure rate of cervical cancer and the survival rate of patients. Cytological examination is currently the most widely used clinical method for screening cervical cancer. In recent years, cytological examination has become the main method for large-scale cervical cancer screening and follow-up observation of high-risk groups, which has greatly reduced the incidence of advanced cervical cancer.

[0003] The traditional method of cytological examination is to use the Pap smear method. The obtained smears have a large number of overlapping cells, and the smears also contain a larg...

Examples

Embodiment 1

[0038] Embodiment 1, an alkaline liquid-based cell preservation treatment solution

[0039] The alkaline liquid-based cell preservation treatment solution consists of the following components and their weight percentages:

[0040] Protein dissolving agent 0.4%, erythrocyte lysing agent 0.01%, NaOH 0.02%, Kathon 0.05% and distilled water 99.52%; Described protein dissolving agent is made up of N-acetyl-L cysteine, urea and dithiothreitol according to The weight ratio is 4:5:3; the erythrocyte lysing agent is composed of saponin, sodium lauryl sulfate and polyethylene glycol octylphenyl ether in a weight ratio of 3:4:4.

[0041] Preparation:

[0042] Mix protein dissolving agent, erythrocyte lysing agent, alkaline substances and preservatives, add appropriate amount of distilled water and stir until completely dissolved, then add distilled water until the pH value is 8, and the product is obtained.

Embodiment 2

[0043] Embodiment 2, an alkaline liquid-based cell preservation treatment solution

[0044] The alkaline liquid-based cell preservation treatment solution consists of the following components and their weight percentages:

[0045] Protein dissolving agent 0.6%, erythrocyte lysing agent 0.05%, NaOH 0.012%, chlorhexidine acetate 0.18% and distilled water 99.158%; Said protein dissolving agent consists of N-acetyl-L cysteine, urea and dithiothreose The composition of alcohol is 5:4:2 by weight; the erythrocyte lysing agent is composed of saponin, sodium lauryl sulfate and polyethylene glycol octyl phenyl ether by weight ratio of 4:3:3.

[0046] Preparation:

[0047] Mix protein dissolving agent, erythrocyte lysing agent, alkaline substances and preservatives, add appropriate amount of distilled water and stir until completely dissolved, then add distilled water until the pH value is 8.6.

Embodiment 3

[0048] Embodiment 3, an alkaline liquid-based cell preservation treatment solution

[0049] The alkaline liquid-based cell preservation treatment solution consists of the following components and their weight percentages:

[0050] Protein dissolving agent 1%, erythrocyte lysing agent 0.1%, KOH 0.001%, domiphene 0.02% and distilled water 98.879%; said protein dissolving agent consists of N-acetyl-L cysteine, urea and dithiothreitol Composed by weight ratio of 6:3:1; the erythrocyte lysing agent is composed of saponin, sodium lauryl sulfate and polyethylene glycol octylphenyl ether in a weight ratio of 5:2:2.

[0051] Preparation:

[0052] Mix protein dissolving agent, erythrocyte lysing agent, alkaline substances and preservatives, add appropriate amount of distilled water and stir until completely dissolved, then add distilled water until the pH value is 9, and the product is obtained.

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