Test paper for rapid diagnosing livestocks schistosomiasis japonica

A technique for schistosomiasis and test strips, which is applied in the biological field, can solve the problems of inability to quickly diagnose bovine schistosomiasis, is not suitable for popularization and use, requires equipment and other problems, and achieves the effects of low cost, wide application range, simple and fast operation

Inactive Publication Date: 2007-09-19
中国农业科学院上海家畜寄生虫病研究所 +2
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  • Description
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AI Technical Summary

Problems solved by technology

Currently, the diagnostic methods for Schistosomiasis japonicum in domestic animals are mainly pathogenic detection methods such as stool examination and immunological detection methods such as IHA, ELISA, and Dot-ELISA. These methods are time-consuming and laborious, or require equipment, and are not suitable for widespread use in the field
In terms of rapid diagnosis of human schistosomiasis, there are related patents and literature reports, but most of these technologies are based on colloid-labeled anti-human antibodies or SPA, and cannot be applied to rapid diagnosis of livestock, especially bovine schistosomiasis

Method used

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  • Test paper for rapid diagnosing livestocks schistosomiasis japonica
  • Test paper for rapid diagnosing livestocks schistosomiasis japonica
  • Test paper for rapid diagnosing livestocks schistosomiasis japonica

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preparation example Construction

[0028] (2) Preparation of anti-SEA antibody

[0029] SEA antigen and 206 adjuvant were mixed in a weight ratio of 1:1, and the New Zealand white rabbit was immunized by intramuscular injection of 1 mg antigen each time. When the concentration reached 1:32 or more, the rabbits were dissected to collect blood, and the serum was collected and separated, purified by ammonium sulfate salting-out method, dialyzed in 4□PBS until no NH4+ or SO42- was found, and the concentration was measured by CBB-SDS method before packaging , stored at -20°C for later use.

[0030] (3) Preparation of buffer solution, blocking solution, stabilizer and homogenizer

[0031] 0.01M phosphate buffer (KH2PO4 0.24g, Na2HPO4·12H2O 17.9g, NaCl 8g, KCl 0.2g, constant volume to 1000ml, adjust pH to 7.4); 0.01MTris-HCl buffer (1.414gTris dissolved in 1000mL deionized water , adjust pH to 7.4 with HCl); blocking solution (3g skimmed milk powder dissolved in 100mL 0.01M Tris-HCl); stabilizer (1gBSA serum albumin...

specific Embodiment 2

[0045] (1) each 5 parts of positive and negative sera of sheep, buffalo, New Zealand white rabbit and mouse are detected with the test strip prepared by the present invention, and the result positive sera all appear red bands at C line and T line, In the negative serum, only a red band appears on the C line (see Figure 4), indicating that the test strip prepared by the double-antigen sandwich method can detect schistosomiasis in various animals.

[0046] (2) Sensitivity and specificity experiments of test strip detection

[0047] 107 positive sheep sera from artificial challenge infection and 80 healthy sheep sera were detected with the test strip of the present invention, and the sensitivity and specificity were 91.6% and 87.5% respectively.

[0048] (3) Cross-reaction test

[0049] 24 sheep sera with fasciola hepatica and 18 bovine sera with trypanosomiasis were detected with the test strip of the present invention, and the result was that the cross-reaction rate with fasci...

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Abstract

The present invention discloses a medical test paper strip for a fast diagnosis of schistosomiasis japonicum in domestic animals. Said medical test paper strip in accordance with the present invention adopts SEA antibody and SEA coated nitrocellulose membrane as a quality control line and a detection line and is manufactured using a double antigen sandwich method, wherein the SEA antigen is labelled by colloidal gold. The present invention has a remarkable specificity and a high sensitivity, and is operated conveniently, simply and quickly. The present invention has a low manufacturing cost and then is suitable for a mass production.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an immunochromatographic test strip for rapidly diagnosing domestic animal schistosomiasis, a preparation method thereof and detection of anti-schistosomiasis antibodies in domestic animals (animals). Background technique [0002] Schistosomiasis is a parasitic disease that seriously endangers the health of humans and animals. Its susceptible animals include humans, cattle, sheep and other livestock and wild animals, involving more than 40 kinds of mammals. Livestock infection with schistosomiasis not only causes heavy losses to animal husbandry production, but more importantly, diseased livestock is also the main source of human schistosomiasis infection. Therefore, the key to control and eradicate schistosomiasis is to do a good job in the detection and treatment of schistosomiasis in livestock. Currently, the diagnostic methods for Schistosomiasis japonicum in domestic animals are...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/558G01N33/52G01N33/532
Inventor 林矫矫彭运潮柴春彦刘金明李浩刘春艳傅志强石耀军陆珂胡述光
Owner 中国农业科学院上海家畜寄生虫病研究所
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