Bone marrow cell culture medium

A technology for bone marrow cells and culture medium, which is applied in bone/connective tissue cells, animal cells, culture process, etc., can solve the problems of few analyzable split images, high cost, complicated preparation process, etc., and achieves good shape and dispersion, Easy to observe, split the effect of many phases

Pending Publication Date: 2020-07-14
浙江百迪生物科技有限公司 +1
View PDF4 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the preparation process of bone marrow chromosomes is complicated, and there are many influencing factors. Phenomena such as few analyzable cleavage images, thick and short chromosomes, and poor dispersion often occur, which greatly reduces the success rate of chromosome karyotype analysis, and the cost is high

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Bone marrow cell culture medium
  • Bone marrow cell culture medium
  • Bone marrow cell culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] The preparation of embodiment 1 bone marrow cell culture medium

[0047] Serum, antibiotics were added to the basal medium. Wherein the basal medium is RPMI-1640 basal medium, and the concentration of each component is as follows:

[0048] Penicillin 100ug / ml

[0049] Streptomycin 50ug / ml

[0050] Solid Serum Replacement 1g / L

[0051] At the same time, the basal medium also contains glutamine, and the concentration of glutamine in it is 3mmol / L.

Embodiment 2

[0052] Embodiment 2 bone marrow cell culture

[0053] Bone marrow cells were cultured according to the following method, and the cultured cells were used for chromosome preparation. The steps that described method comprises are:

[0054] (1) Preparation of bone marrow cell culture medium: configure according to the components and dosages described in Example 1;

[0055] (2) Kind of bone marrow: Inoculate 0.5ml of bone marrow cell puncture solution into a glass or plastic bottle containing 10ml of medium in step (1), and gently rotate the culture tube to mix;

[0056] (3) Cultivation: place the culture medium inoculated with bone marrow cells in a carbon dioxide incubator at 37 degrees Celsius for cultivation, and the cultivation time is 72 hours;

[0057] (4) Terminate the culture: add colcemid with a final concentration of 0.1 ug / ml to the culture medium, and let stand for 15-30 minutes to terminate the culture.

Embodiment 3

[0058] Example 3 Bone marrow cell chromosome preparation

[0059] Carry out bone marrow cell chromosome preparation according to the following method, and the prepared cells are used for chromosome karyotype analysis. The steps that described method comprises are:

[0060] (1) Cell collection: transfer the cells terminated in Example 2 to a centrifuge tube, and remove the supernatant after centrifugation for 5 minutes under the action of a centrifugal force of 500 g to collect the cells;

[0061] (2) Hypotonic treatment: add 5-10ml of 0.075M KCL solution to the cells collected in step (1) to suspend the cells, and incubate at 37 degrees Celsius for 10-12 minutes;

[0062] (3) Centrifugation: centrifuge the cell suspension after the hypotonic treatment in step (2), and discard the supernatant after centrifugal force of 500g for 5 minutes;

[0063] (4) Fixation: Add 5-10ml of freshly prepared, pre-cooled acetic acid:methanol=1:3 fixative to the centrifuge tube, and fix at 4°C fo...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
concentrationaaaaaaaaaa
Login to view more

Abstract

The invention discloses a bone marrow cell culture medium. Antibiotics and solid serum substitutes are added to a basal culture medium. The bone marrow cells cultured by the culture medium provided bythe invention can be used for chromosome preparation, and the culture medium shows the advantages of good chromosome presentation state, easiness in observation and the like, and has wide applicationprospect in the field of medical detection.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a bone marrow cell culture medium, in particular to a bone marrow cell culture medium for chromosome karyotype analysis. Background technique [0002] People use various methods and different dyes to process chromosome samples to make each chromosome appear light and dark, or the technology of different shades of banding is called banding technique (banding technique). Since the 1970s, the banding technology has been greatly developed, and the G band is mainly developed after being dyed with Giemsa dye, and the displayed banding pattern is distributed on the entire chromosome, so it is the most popular banding technology at present. (Q belt, G belt, C belt, R belt, T belt), G belt is a widely used belt type. [0003] Chromosome karyotype analysis takes metaphase chromosomes as the research object, and analyzes, compares, sorts and numbers chromosomes with the help of banding technol...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/077G01N1/28
CPCC12N5/0669G01N1/2813C12N2500/32
Inventor 张海峰彭昉俞英豪张孝松陆诚
Owner 浙江百迪生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap