Test strip for testing psychrophiles in chilled foods and preparation method thereof

Abstract

The invention discloses a test strip for testing psychrophiles in chilled foods and a preparation method thereof, which belong to the technical field of biosensors. The test strip comprises a substrate, a transparent membrane, a reaction membrane, a glass fiber piece and polyester plates, wherein the glass fiber piece, the reaction membrane and the transparent membrane are sequentially and fixedly arranged between the polyester plates and the substrate from top down; the two ends of the glass fiber piece are connected with the inner sides of two polyester plates respectively; the outer sides of the polyester plates are fixedly connected with the substrate; and the center of the substrate is provided with a through hole just opposite to the transparent membrane. The test strip has high sensitivity, high accuracy, good repeatability and a wide application range and can be effectively preserved for more than one year.

Description

technical field

[0001] The invention relates to a product in the technical field of biosensing, in particular to a test strip for testing psychrophilic bacteria in refrigerated food and a preparation method thereof. Background technique

[0002] Psychrophilic bacteria is a kind of bacteria with strong antifreeze ability. Generally, it is most suitable for growth between 0°C and 20°C. Therefore, it brings certain difficulties to low-temperature storage of food, such as meat, milk and dairy products, soy products, salads, etc. And aquatic products, etc., are more susceptible to its contamination of the food. Most psychrophilic bacteria have been generally recognized worldwide as pathogens of zoonotic and foodborne diseases in etiology. At present, the methods for detecting psychrophilic bacteria are mainly traditional bacterial identification methods and immunological detection methods such as PCR and ELISA. The traditional bacterial identification methods are cumbersome and ...

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