Fixation fluid for saving cast-off cells and manufacturing method of cell shallow layer smear

Abstract

The invention discloses a preservation solution for exfoliated cell and a preparation method which uses the preservation solution to process cell thin layer smear. The preservation solution for exfoliated cell comprises the components which are prepared by the volume proportion of 10 to 15 portions of methanol, 10 to 15 portions of alcohol, 1 to 2 portions of glycerin, 1 to 2 portions of glycol polyethylene, 10 to 12 portions of formaldehyde, 0.5 to 1 portions of glacial acetic acid, 10 to 20 portions of sodium chloride with 0.85 percent to 0.9 percent of mass concentration and 33 percent to 68.5 percent of phosphate buffer. The steps of the preparation method for cell thin layer smear are that: 1) the collected sample is put in the preservation solution for exfoliated cell for shaking; the sample is transferred to a centrifuge for centrifugal treatment after standing; 2) the supernatant is removed until 1ml to 2ml residue is left in the centrifuge tube; the cell suspension is obtained after uniform vibration; 3) 0.5ml to 1ml cell suspension is put into a smear preparation cup which is made into cell thin layer smear by a cell smear preparation machine.

Description

(1) Technical field: [0001] The invention relates to a cell preservation and fixation solution, in particular to the exfoliated cell preservation and fixation solution; the invention also relates to a method for making a cell thin-layer smear treated with the preservation and fixation solution. (two) background technology: [0002] Living cells have physiological functions, biological activities, and inherent cell morphology and structure, and have application value in many aspects. However, if it is placed away from the original living environment, the physiological function and biological activity of the cells, as well as the shape and structure of the cells will change. The conventional method is to cryopreserve the cells. However, cryopreservation requires the purchase of special freezing equipment, and the freezing and thawing processes will inevitably cause significant damage to the cells, resulting in changes in cell morphology and structure. Therefore, cryopreservat...

AI Technical Summary

Problems solved by technology

[0005] There are two kinds of techniques for making cell thin-layer smears: one is liquid-based thin-layer cell technology (TCT technology), which is to add the obtained cells into the preservation solution, and directly absorb and transfer the cells through the machine. Making thin-layer cell smears on glass slides has the advantage of automatic machine production, but the disadvantages are that only one slice can be produced each time, and the production time is long, the machine cost is high, and the maintenance is complicated; the second is LPT technology, which is to suspend cells The liquid is directly dropped on the glass slide to make a smear. Its advantage is that no special equipment is needed and the operation is simple. The disadvantage is that all the obtained cells are not used to make a smear, which may easily cause cell overlap, which is not conducive to observation and diagnosis.