The invention belongs to the technical field of air purification materials, and particularly relates to a natural terpene type nanocomposite material capable of releasing negative oxygen ions, and a preparation method of the natural terpene type nanocomposite material. The natural terpene type nanocomposite material capable of releasing the negative oxygen ions is prepared from 1-40 parts of cinnamomum camphora, 1-90 parts of pine pollen, 1-60 parts of beeswax, 1-70 parts of uncaria rhynchophylla, 1-30 parts of lotus leaves, 1-70 parts of pine needles, 1-40 parts of Chinese mugwort and 1-200 parts of substrates. The preparation method comprises the following steps of (1) weighing the cinnamomum camphora, the pine pollen, the beeswax, the uncaria rhynchophylla, the lotus leaves, the pine needles and the Chinese mugwort, performing mixing, performing grinding, adding deionized water, performing distillation, performing extraction, and performing separation so as to obtain terpene type substances; (2) weighing the substrates, grinding the weighed substrates, and activating the ground substrates; and (3) adding the terpene type substances obtained in the step (1) to the activated substrates obtained in the step (2), performing mixing, and performing grinding so as to obtain the natural terpene type nanocomposite material. The natural terpene type nanocomposite material capable of releasing the negative oxygen ions is scientific, natural and healthy in formula, can sufficiently release the negative oxygen ions, can promote metabolism of human bodies, can enhance immunity and has the functions of bacteriostasis, sterilization and deodorization.