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Tyrosine ammonia lyase recombinant bacterium and construction method and application thereof, and tyrosine ammonia lyase and preparation method and application thereof

A technology of tyrosine ammonia lyase and recombinant bacteria, applied in botany equipment and methods, biochemical equipment and methods, applications, etc., can solve the limitation of phenylpropane metabolism and limit the application and development of phenylpropanoid active compounds and other problems, to achieve the effect of high-efficiency catalysis, good stability, and easy production

Inactive Publication Date: 2020-03-31
杭州唯铂莱生物科技有限公司
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

Due to the limitation of cinnamic acid content in nature, the metabolism of biotransformation phenylpropane is limited, which further limits the application development work of phenylpropanoid active compounds

Method used

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  • Tyrosine ammonia lyase recombinant bacterium and construction method and application thereof, and tyrosine ammonia lyase and preparation method and application thereof
  • Tyrosine ammonia lyase recombinant bacterium and construction method and application thereof, and tyrosine ammonia lyase and preparation method and application thereof
  • Tyrosine ammonia lyase recombinant bacterium and construction method and application thereof, and tyrosine ammonia lyase and preparation method and application thereof

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Embodiment Construction

[0024] The present invention will be described in more detail below in conjunction with the accompanying drawings. It should be noted that the following description of the present invention with reference to the accompanying drawings is only illustrative rather than limiting. Different embodiments can be combined with each other to form other embodiments not shown in the following description.

[0025] The present invention also provides a recombinant plasmid comprising one or two tyrosine ammonia lyase expression genes as shown in SEQ ID NO.1 or SEQ ID NO.2.

[0026] The present invention provides a tyrosine ammonia lyase recombinant bacterium, the tyrosine ammonia lyase recombinant bacterium has a recombinant plasmid with a nucleotide sequence as shown in SEQ ID NO.1 or SEQ ID NO.2, that is to say , the tyrosine recombinant bacterium contains the above one or two recombinant expression plasmids for expressing the above one or two tyrosine ammonia lyases.

[0027] Specifical...

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Abstract

The invention provides a tyrosine ammonia lyase recombinant bacterium and a construction method and application thereof, and tyrosine ammonia lyase and a preparation method and application thereof. The construction method of the tyrosine ammonia lyase recombinant bacterium comprises the following steps: extracting a target gene with a nucleotide sequence shown in SEQ ID NO.1 or SEQ ID NO.2; connecting the target gene to a plasmid to obtain a recombinant plasmid; introducing the recombinant plasmids into a thallus to obtain a tyrosine ammonia lyase recombinant bacterium. The invention aims at providing a tyrosine ammonia lyase recombinant bacterium and a construction method and application thereof, and tyrosine ammonia lyase and a preparation method and application thereof. The tyrosine ammonia lyase is high in conversion efficiency and high in stability.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a tyrosine ammonia lyase recombinant bacterium, a tyrosine ammonia lyase and a preparation method and application thereof. Background technique [0002] TAL (Tyrosine ammonia lyase, tyrosine ammonia lyase) is an important key enzyme for the conversion of phenylpropanoid precursors, which can efficiently convert tyrosine into cinnamic acid and realize further product applications. TALs are found in monocotyledonous plants such as sorghum, barley, wheat, oats, rice, corn, sweet sugar cane, and parsley. Basidiomycetes, Capsulated Rhodobacter, Actinomycetes, Rhodotorula viscosus and Trichosporon have been found in microorganisms. Due to the limitation of cinnamic acid content in nature, the metabolism of biotransformation phenylpropane is limited, and further limits the application and development of phenylpropanoid active compounds. Therefore, it is necessary to develop an enzyme that...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/21C12N15/60C12N15/70C12N9/88C12P7/42C12R1/19
CPCC12N9/88C12N15/70C12P7/42C12Y403/01023
Inventor 陶福平秦勇张炜占纪勋
Owner 杭州唯铂莱生物科技有限公司
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