Microbial viable bacteria combination and preparation method thereof

A technology of microorganism and production method, which is applied in the field of microbial live bacteria combination preparation and its preparation, can solve the problems affecting human public health and safety, the decline of animal self-immunity, human health hazards, etc., so as to reduce the cost of raw materials and the consumption of energy and power. , the effect of improving animal immunity and improving environmental quality

Inactive Publication Date: 2007-02-07
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, it was later discovered that the long-term use and abuse of antibiotics in feed has caused other worrying problems; the resistance of pathogenic bacteria has been significantly enhanced...

Method used

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  • Microbial viable bacteria combination and preparation method thereof

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Embodiment 1

[0038] Embodiment one: the total number of viable bacteria is greater than the preparation of 5 billion / ml water preparation products

[0039] In a 70L fermenter, a 50L culture medium was formulated with 3% glucose, 0.4% ammonium sulfate, 0.2% yeast extract, 0.02% dipotassium hydrogen phosphate, and 0.02% magnesium sulfate. Sterilize according to conventional methods and cool to 37°C, inoculate an eggplant bottle with Bacillus subtilis production strains at a culture temperature of 37°C and a rotation speed of 250 rpm; after culturing for 18 hours, inoculate an eggplant bottle with Rhodotorula viscosus production strains species, the culture temperature was lowered to 28°C, and the joint culture was put into the tank after 20 hours. Add water to dilute to a suspension with a total viable count of more than 5 billion / ml, add 0.1% sodium benzoate as a preservative, and pack to obtain the product. The total number of live bacteria in this product is more than 5 billion / ml, of wh...

Embodiment 2

[0040] Embodiment two: total viable count is greater than the preparation of 10 billion / gram powder product

[0041] In a 70L fermenter, a 45L culture medium was formulated with 4.0% sucrose, 0.5% corn steep liquor, 0.3% urea, 0.02% dipotassium hydrogen phosphate, 0.02% magnesium sulfate, and 0.2% sodium chloride. Sterilize according to conventional methods and cool to 35°C, inoculate 1 eggplant bottle with Bacillus subtilis production strains at a culture temperature of 36°C with a rotation speed of 220 rpm; after 24 hours of cultivation, inoculate 2 eggplant bottles with Rhodotorula viscosus production strains species, the culture temperature was lowered to 30°C, and the joint culture was put into the tank after 76 hours. Add an appropriate amount of corncob powder to absorb and adjust the total number of viable bacteria to more than 10 billion / g, and then pack it after boiling and drying to obtain the product. The total number of viable bacteria in this product is more tha...

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Abstract

the invention discloses microbe viable bacteria combined agent and preparing method, which consists of Bacillus subtilis and Rhodotorula glutinis, wherein the Bacillus subtilis is seeded firstly and then Rhodotorula glutinis, which need 15-100 h as combined culturing time.

Description

Technical field: [0001] The invention relates to a combination preparation of living microorganisms and a preparation method thereof, belonging to the technical field of microbial fermentation engineering. Background technique: [0002] Since the discovery in the middle of the last century that adding antibiotics to feed has obvious antibacterial and growth-promoting effects, antibiotics and other pharmaceutical feed additives have played a huge role in preventing animal diseases, improving animal production performance and economic benefits, and promoted the livestock and poultry breeding industry, especially It is the development of intensive farming. However, it was later discovered that the long-term use and abuse of antibiotics in feed has caused other worrying problems; the resistance of pathogenic bacteria has been significantly enhanced, the animal's own immunity has declined, drug residues have caused human health hazards, and excrement has caused environmental poll...

Claims

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Application Information

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IPC IPC(8): C12N1/00C12N1/20C12N1/16A23K1/16C12R1/125A23K10/18
Inventor 施跃峰李孝辉竺利红吴吉安梁建根陈兵桑金隆
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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